Chromatographic systems for size exclusion chromatography (SEC) are provided that comprise an inlet, an outlet, an analytic column having a first interior volume that has a first length and a first cross-sectional area normal to the first length, the first interior volume containing a first stationary phase, and a guard column having a second interior volume that has a second length and a second cross-sectional area normal to the second length, the second interior volume containing a second stationary phase. The inlet is in fluid communication with the guard column, the guard column is in fluid communication with the analytic column, and the analytic column is in fluid communication with the outlet. Moreover, the second length is smaller than the first length, and the second cross-sectional area is smaller than the first cross-sectional area.

A monolithically integrated chromatography device includes a first chromatography column and a second chromatography column formed in a common substrate. The first chromatography column and the second chromatography column are each in fluidic communication with a port formed on the same common substrate. When the port is open, the first chromatography column and the second chromatography column are in fluidic communication with one another. When the port is closed, the first chromatography column and the second chromatography column are not in fluidic communication with one another. The monolithically integrated chromatography device can include a heating element formed on the same substrate as the chromatography columns.

Exemplary embodiments integrate a tee or valve into an outlet end fitting of a liquid chromatography column. The tee or valve is suitable for providing additional fluidic flow paths to ports of the end fitting and eliminates the need for post-column fluidic conduits connecting to tees or valves to insert fluidic inputs or divert flow to outputs. This integration decreases the distance that eluent from the liquid chromatography column has to travel to reach a detector relative to systems that use external tees or valves while providing tee/valve functionality and reducing the fluidic volume post-column. As a result, the exemplary embodiments help decrease sample dispersion.

To analytically determine concentration of dissolved gases in a water pipeline, a water sample is drawn from a source carrying water with dissolved gas, through a water source port of a four-way valve. The water sample is flowed from the water source port towards a syringe port of the valve and into a syringe fluidically coupled to the syringe port to hold the water sample. Inert gas is drawn through an inert gas port of the valve from an inert gas source and is flowed from the inert gas port towards the syringe port and into the syringe. A mixture of the water sample and the inert gas is flowed from the syringe port towards an analyzer port of the valve and into an analyzer fluidically coupled to the analyzer port.

Provided herein are devices and methods for selecting and stimulating a plurality of cells in a sample of cells using column chromatography. In some aspects. provided is a device comprising a temperature control member to provide heat to a chromatography stationary phase and a connector configured to provide air to the stationary phase during column chromatography. In some aspects. the devices and methods provided herein reduce the time needed to generate a population of selected and stimulated cells useful for genetic engineering. and ultimately, cell therapy. compared to existing devices and methods.

In this invention, chromatography is integrated on a centrifugal platform to enable low-cost automated purification. Differing from the traditional chromatography method, purification and separation of a centrifugal compound collecting platform disclosed in the present invention mainly uses a centrifugal force to drive the fluid to flow outward in the radial direction when the motor rotates. The compounds to be separated react with the column packing during the flow, and the compounds with different polarities in the sample are gradually separated. The flow of the fluid can be governed by the motor and the geometry of the fluidic design such that compounds with different characteristics can be separated and collected in different collecting chambers.

Described herein is an apparatus and method for operating said apparatus which may allow for the repeat analysis of a fluid sample. Said apparatus may comprise an inlet segment, an analysis segment, an outlet segment, a bypass segment, a holding segment and fluidic switches, wherein fluidic communication may be established between the holding inlet segment, analysis segment, outlet segment, bypass segment and holding segment.

A coupling unit for coupling gas conduits in a gas distribution network comprises a base part and a module part. The base part comprises at least one base gas orifice, while the module part is provided with at least one module gas orifice, each module gas orifice matching a corresponding base gas orifice. Each base gas orifice is provided with a valve which is normally closed. A module gas orifice is configured for opening the valve of the corresponding base gas orifice when the base part and the module part are brought together. Each valve may comprise a movable valve element accommodated in a respective base gas orifice and a resilient element for keeping the valve normally closed.

A liquid chromatography apparatus includes a diffusion-bonded separation column comprising a lower substrate comprising titanium, an upper substrate comprising titanium, and a titanium patterned foil disposed between the lower substrate and the upper substrate. The lower substrate, titanium patterned foil, and upper substrate are diffusion bonded together to form a fluid path extending from an inlet port to an outlet port, wherein walls defining the fluid path within the diffusion-bonded separation column include a titanium surface coating.

The field of chromatography, specifically the field of expanded bed chromatography. The invention is an expanded bed chromatography apparatus 1 which has an elongate tube 4 defining an operating volume 8 and having a central axis 7. The top end of the tube is sealed with a cap 2 having an exit port 3. The other end of the tube 4 is connected to a base 5. The base includes a process fluid inlet having a plurality of outlet apertures 10a, 10b, 10c in fluid communication with the operating volume of the tube and an inlet aperture 11 in fluid communication with the plurality of outlet apertures. Each of the plurality of outlet apertures is spaced radially from the central axis of the tube. In use, a mobile phase of process fluid passes up through a particulate media in the tube from the inlet to the exit port in a cap at the top of the apparatus. Target product is adsorbed onto the particulate media and is subsequently eluted from it. Alternatively, impurities are adsorbed on the particulate media and the target solution passes through the expanded bed for collection.