The invention relates to tubing assemblies and methods for forming a fluidic connection to make the same. The assemblies include an outer tube having disposed therein a first inner tube affixed to the outer tube, a second inner tube and a support tube disposed in the second inner tube. The second inner tube is abutted against the first inner tube. A radial crimp is made in the outer tube over the second inner tube a distance x from where the tubes abut; the distance x being such that the end of the second inner tube seals against the end of the first inner tube. Various embodiments provide assemblies suitable for use with high fluidic pressure (e.g., greater than an about 70 MPa, approximately about 10,000 psi) and formation of chromatographic column assemblies comprising a capillary tube predisposed within the first or second inner tube prior to formation of the seal.

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge.

A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port, a cartridge for input to the cartridge receiving port having a sample input and a channel, where the channel includes a mixture of Raman-scattering nanoparticles and a calibration solution, where the calibration solution includes chemical compounds capable of interacting with a sample under test input to the cartridge and the Raman-scattering nanoparticles, and an optical detection system in the housing, where the optical detection system is capable of providing an illuminated electric field, where the illuminating electric field is capable of being used for Raman spectroscopy with the Raman-scattering nanoparticles and the calibration solution to analyze the sample under test input to the cartridge.

A vial cap for removing a matrix component from a liquid sample is described. The vial cap includes a cap body, an inlet portion, and an outlet portion. The cap body is configured to have a slidable gas and liquid seal with a side wall of a sample vial. The inlet portion includes a counterbore section that holds a filter plug. The filter plug includes a polyethylene resin and a material selected from the group consisting of an ion exchange material and a reversed-phase material. The vial cap is adapted for solid phase extraction for use in an autosampler with a plurality of sample vials.

A method for separating at least one target compound from a feed solution is provided. The method includes filling a bioprocess package with a chromatography resin. The bioprocess package includes a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet. The method further includes flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin, washing the chromatography resin in the bioprocess package, and eluting the chromatography resin.

A method of making and loading a chromatography column includes selecting a column tube having an appropriate elasticity, and inner diameter and length, selecting appropriately sized flow distributors, a second flow distributor having a diameter that is larger than the inner diameter of the tube, permanently securing a first flow distributor to a first end of the tube, loading packing medium into the tube, inserting the second flow distributor into a second end of the tube to drive the second flow distributor into the tube to form a sealed chamber within the tube, adjusting the longitudinal position of the second flow distributor within the tube by applying a force to the second flow distributor until it reaches a desired location within the tube, and/or forcing liquid into the chamber to move the second flow distributor and permanently securing the properly positioned second flow distributor within the tube.

The invention relates to tubing assemblies and methods for forming a fluidic connection to make the same. The assemblies include an outer tube having disposed therein a first inner tube affixed to the outer tube, a second inner tube and a support tube disposed in the second inner tube. The second inner tube is abutted against the first inner tube. A radial crimp is made in the outer tube over the second inner tube a distance x from where the tubes abut; the distance x being such that the end of the second inner tube seals against the end of the first inner tube. Various embodiments provide assemblies suitable for use with high fluidic pressure (e.g., greater than an about 70 MPa, approximately about 10,000 psi) and formation of chromatographic column assemblies comprising a capillary tube predisposed within the first or second inner tube prior to formation of the seal.

A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.

A pipe for a chromatograph includes a thin portion and a thick portion. The thin portion extends in one direction. The thick portion is located at an end portion of the thin portion in the one direction. The thin portion and the thick portion are integrally formed of a single member. The outer diameter of the thin portion is smaller than the outer diameter of the thick portion. The pipe is used in a chromatograph.

A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.