The present invention relates to a flow-through device comprising at least one separation column wherein a first packing component, which comprises particles of alumina and/or silica, and a second packing component, which comprises a powder of one or more hygroscopic salts are provided. The two packing components may be blended or layered in the device, which may comprise a single tube or a plurality of tubes arranged in a plate format, such as the wells of a multiwall plate or tubes in a rack. In addition, the invention relates to a method for removing one or more matrix components, such as pigments, from a biological sample, by passing said sample across a first packing component, which comprises particles of alumina and/or silica, and a second packing component, which comprises a powder of one or more hygroscopic salts.

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

A fractal flow device comprising at least one fractal pack. The at least one fractal pack comprises at least two fractal cells, where each fractal cell comprises a fractal distributor, a chamber adjacent the fractal distributor, and a fractal collector adjacent the chamber. Methods of using the fractal flow device are also disclosed.

Disclosed is a separation column for micro gas chromatography, having superior separation performance and including a microchannel formed on a substrate and having a serpentine shape, and bumps formed on the surface of the microchannel, wherein the bumps are alternately disposed to face each other on the surface of the microchannel. In the separation column for micro gas chromatography, a sufficient pressure drop occurs, and thus an interaction between a gas mixture to be analyzed and a stationary phase in the column sufficiently takes place, whereby individual gas components are efficiently separated from the gas mixture, discharged from the column, and detected.

A cyclic chromatographic purification method for the isolation of a product from a feed mixture consisting of the product and at least one further component representing impurities, which impurities bind stronger to the chromatographic stationary phase than the product is given. The method uses at least two chromatographic adsorbers as chromatographic stationary phase, grouped into only one first adsorber section (1) and one second adsorber section (2), wherein if an adsorber section comprises more than one chromatographic adsorber these are permanently connected in series, wherein the first adsorber section (1) has a first adsorber section inlet and a first adsorber section outlet, and the second adsorber section (1) has a second adsorber section inlet and a second adsorber section outlet.

The present invention comprises a novel multimodal chromatography sequence of short length alternating adsorption and size exclusion media operating with gradient elution. The novel multimodal chromatography in an oscillating series utilizes the alternating solvent exchange media to reposition the active region of separation back in phase with the target solutes. Each solvent exchange column bed length in the sequence is designed to achieve a subtle decrease or increase in the solvent gradient (or salt gradient) concentration associated with the two solutes of interest which results in an extension of the active separation or increasing differences in solute velocity for two solutes of interest. The novel oscillatory chromatographic system demonstrates much improved separation capability as shown by a one dimensional model.

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel.

Adsorptive bed devices include a monolithic scaffold having a stress absorbing rigid structure and open cells filled with adsorptive beads. The monolithic scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal.

Additively manufactured monolithic structures for containing and directing flows of fluids are described herein, which can achieve high contact area between fluids and solids while maintaining uniform flow conditions and requiring low applied pressures to yield desired flow rates, for use in heat exchangers, chromatography columns, catalytic converters, etc. An exemplary monolithic structure comprises a plurality of tiled unit cells having a same shape, where the tiled unit cells are integrally formed as a single component. The tiled unit cells are arranged to define one or more interior regions of fluid flow, one or more inlets to each interior region of fluid flow, and one or more outlets to each interior region of fluid flow. The structures and methods of tiling herein are suited to additive manufacturing technologies such as projection stereolithography, multiphoton lithography, etc.

A sample cartridge for a liquid chromatography device includes a microfluidic chip. A collector in the microfluidic chip includes a collector flow channel and a first window for acquisition of spectral data from a sample in the collector flow channel.