The present invention provides a high throughput method to quantify and characterize the size and integrity of viruses and viral molecules. In one embodiment, the present invention provides a method to quantify and characterize size and integrity of Foot and Mouth Disease virus (FMDV) using chromatographic system and in-line Dynamic Light Scattering (DLS) technique. In one embodiment, the present invention further comprises a column-switching system for running multiple analyses simultaneously. The present invention also provides a method to develop and evaluate FMDV containing products for preventing Foot and Mouth Disease (FMD). In one embodiment, the methods described herein assess the stability of FMDV. In another embodiment, the methods described herein serve as in-process quality control for a manufacturing process of FMD vaccine.

To provide a gas chromatograph allowing a substance led out from a measurement target component to reach a reduction reaction part and capable of preventing occurrence of a quantification error while preventing corrosion and sensitivity deterioration of a metal pipeline due to measurement non-related components such as halogen, sulfur and the like. The gas chromatograph includes: an oxidation reaction part into which a sample gas passed through a column is introduced and which oxidizes the measurement target component and converts the resultant measurement target component to a prescribed intermediate component to be led out therefrom; and an intermediate gas flow path for deriving the intermediate component from the oxidation reaction part, and in this configuration, a reagent to be reacted with measurement non-related components is provided in the intermediate gas flow path, and the reagent contains a metal element to be reacted with only oxygen.

In order to provide a gas chromatograph in which any adverse effects on a reduction reaction unit that are caused by the degradation of a reduction catalyst, and any adverse effects on an analyzing unit are prevented, a gas chromatograph is provided with: a column that, by causing a sample gas to pass through itself, is able to separate constituents for measurement that are contained in the sample gas; an oxidation reaction unit that uses an oxidizing gas to oxidize the sample gas that has passed through the column so as to create an oxidation sample gas; a reduction reaction unit that uses a reducing gas to reduce the oxidation sample gas created by the oxidation reaction unit so as to create an oxidation reduction sample gas; and an analyzing unit that analyzes the sample gas.

Aspects of the present disclosure include a solid phase sorbent for preparation of analytical samples. The solid phase sorbent includes particles that are surface modified with an -cyclodextrin moiety. Also provided is a method of reducing matrix effects in an analytical sample. In some embodiments, the method includes contacting a sample comprising a matrix-interfering agent and an analyte with -cyclodextrin modified particles to produce a contacted sample wherein the matrix-interfering agent binds to the -cyclodextrin modified particles; separating the -cyclodextrin modified particles from the contacted sample to produce a matrix-reduced composition; and detecting the analyte in the matrix-reduced composition. Systems for practicing the subject methods are provided that include the subject solid phase sorbent.

A pre-analysis treatment device usable for an amino acid, organic acid, and glucide includes an ion-exchange unit configured to load a test sample on a solid-phase cartridge S having a strong ion-exchange resin phase, to allow the strong ion-exchange resin phase to adsorb a predetermined organic compound, then supply a dehydration solvent to dehydrate the strong ion-exchange resin phase, and a derivatization unit configured to feed a predetermined amount of the derivatization reagent to the dehydrated strong ion-exchange resin phase to allow the derivatization reagent to retain for a predetermined time period, thereby trimethylsilylating the organic compound adsorbed on the strong ion-exchange resin phase, and simultaneously desorbing the trimethylsilylated organic compound from the strong ion-exchange resin phase, and then supply a push-out solvent to push the trimethylsilylated organic compound desorbed, out of the solid-phase cartridge S. The device enables at least one organic compound selected from amino acids, organic acids and glucides contained in a test sample to be derivatized and collected easily in a short period of time, and automation of the pre-analysis treatment.

The present invention provides a high throughput method to quantify and characterize the size and integrity of viruses and viral molecules using chromatographic system and in-line Dynamic Light Scattering (DLS) technique. In one embodiment, the present method quantifies and characterizes the size and integrity of enveloped or non-enveloped virus, live or live-attenuated or inactivated virus, recombinant viral vectors, or virus-like particles (VLPs). In one embodiment, the present invention comprises a column-switching system for running multiple analyses simultaneously. The present invention also provides a method to develop and evaluate virus containing products for the prevention of viral diseases. In another embodiment, the methods described herein serve as in-process quality control for manufacturing processes of virus vaccines.

Provided is a system comprising a device that performs one or more reactions to liquid or supercritical fluid chromatograph effluents and produces molecules that are subsequently detected by a suitable detector. This allows for one to practice a method for the detection and quantification of organic molecules from a liquid chromatograph for the purpose of increasing detection limits and allowing for the universal detection of organic molecules. The linear dynamic range and molecular response are greater than those previously available.

The utilization of a specific preconcentration protocol within an automated, on-line drinking water analysis method to amplify detection levels of individual halo acetic acids is provided. The proposed sequential injection analysis procedures allow for measuring such levels in drinking water samples from remote locations with post-column reaction of ion chromatography (PCR-IC). The novel remote preconcentration method injects such known concentrations via a syringe at regular intervals in a manner to provide a baseline measurement that accords a reliable comparison with the unknown amounts present within the drinking water samples at issue. In such a manner, the on-line, remote system provides the necessary reliability for a water utility or like entity on which to base any further needed water treatment activities without having to perform such measurements in a distinct lab setting.

Systems and methods that facilitate the automatic (or substantially automatic) preparation of a sample of a product containing molecules for analysis and automatic (or substantially automatic) performance of an assay of that sample. Thus, the preparation and analysis can be performed substantially in-real time, or, in other words, much more quickly than presently allowed by conventional systems and methods.