The present disclosure relates to methods of modifying complex extracts such that components or mixtures of components are selectively removed or added, thus providing a complex mixture that does not naturally occur with a refined or a tuned therapeutic or nutraceutical effect. In various aspects, the complex extract can be an extract obtained from one or more plants, e.g., an extract obtained from green tea leaves. The present disclosure pertains to compositions obtained by the disclosed methods, nutraceutical compositions comprising same, pharmaceutical compositions comprising same, and methods of treating various conditions, including physiological dysfunctions associated with elevated reactive oxygen species and/or inflammatory molecule, e.g., TNFα, expression using same. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

A controller (50) of a liquid chromatograph (1) is configured to execute, as an analysis operation in an analysis unit (3), a sample injection step of bringing a high-pressure valve (10) into a loading state, sucking a sample from a tip of a needle (12) to hold a sample in a sampling channel (2), then connecting the sampling channel (2) to an injection port (16) and bringing the high-pressure valve (10) into an injecting state, and supplying a mobile phase from a liquid supplier (6), thereby injecting a sample held in the sampling channel (12) into an analysis channel (4), and an analysis step of separating components of a sample injected into the analysis channel (4) in a separation column (14) by bringing the high-pressure valve (10) in the loading state and supplying the mobile phase from the liquid supplier (6) after the sample injection step is ended. In a case where at least a predetermined condition is satisfied, after the analysis step is ended, the controller is configured to execute, as the analysis operation, a system cleaning step of cleaning a liquid flowing route from the sampling channel (2) to the analysis channel (4) by connecting the sampling channel (2) to the injection port (16) and bringing the high-pressure valve (10) into the injecting state, and supplying the mobile phase and/or a cleaning liquid from the liquid supplier (6).

An analysis apparatus includes an extraction unit that extracts a baseline portion from chromatographic data that is acquired from an apparatus, and a determination unit that determines a normality in the apparatus based on normal chromatographic data and a feature of the baseline portion of the chromatographic data extracted by the extraction unit.

A waveform processing assistance device includes an acquirer that acquires a correspondence relationship between a plurality of values of a waveform processing parameter and a plurality of results of waveform processing as a plurality of peak separation information pieces in regard to a plurality of peaks that are separated from one or a plurality of waveform data pieces based on the plurality of values of the waveform processing parameter, a determiner that determines robustness of each value of the waveform processing parameter based on a plurality of peak separation information pieces acquired by the acquirer, and a display controller that causes the display to display a plurality of peak separation information pieces acquired by the acquirer and a robustness information piece representing robustness of each value of the waveform processing parameter that is calculated by the determiner.

A first reference line that is a regression line obtained from data within a predetermined range including a starting point of a peak detected from data of a graph showing changes in intensity with respect to a parameter, a second reference line that is a regression line obtained from data within a predetermined range including a ending point of the peak, and a third reference line connecting the starting and ending points, and one or more intermediate control points in a triangle defined by the first, second, and third reference lines are determined; and a Bezier curve between the starting point and the ending point is created to be determined to be a baseline of the peak, the Bezier curve being determined by control points of the starting point, the one or more intermediate control points, and the ending point in order on a parameter axis.

A method includes: performing a time-frequency analysis on measurement data to obtain waveform data representing a temporal change in the intensity of each of a plurality of frequency components; dividing the waveform data of each of a plurality of predetermined frequencies into a plurality of segments so that each section where positive values successively occur and each section where negative values successively occur in a time-axis direction are defined as one segment; calculating the area of each of the segments to obtain segment values; creating, for the waveform data of each of the predetermined frequency components, a selected segment group by excluding a segment whose segment value exceeds a predetermined reference value from the segments in the waveform data; and determining a noise level of each of the predetermined frequency components based on the average value of the segment values of the segments included in the selected segment group.

A chromatographic data system processing apparatus performs data processing based on plot data measured by a chromatograph. The chromatographic data system processing apparatus includes a virtual curve calculation portion which obtains a virtual curve based on the measured plot data, a tentative feature point acquisition portion which obtains a tentative feature point based on the obtained virtual curve, and an actual plot data feature point extraction portion which extracts an actual plot data feature point corresponding to the tentative feature point from the measured plot data.

To reduce an arithmetic processing load or an influence of noise at the time of virtual curve calculation processing, provided is a data processing device for a chromatograph, which is configured to execute data processing based on plot data measured by using a chromatograph, the data processing device including a virtual curve calculation data generator configured to obtain a smaller number of pieces of virtual curve calculation data than a number of pieces of the measured plot data; and an arithmetic processor (163) functioning as a virtual curve calculator configured to obtain a virtual curve based on the virtual curve calculation data.

A sample chromatogram produced by measurement of a mobile phase into which a sample has been injected is acquired by a sample chromatogram acquirer. A background chromatogram produced by measurement of a mobile phase into which a sample is not injected or a mobile phase into which a control sample is injected is acquired by a background chromatogram acquirer. Alignment of a sample chromatogram and a background chromatogram is performed by an alignment processor by causing of baselines of the acquired sample chromatogram and the acquired background chromatogram to coincide with each other. Subtraction processing of subtracting a background chromatogram from a sample chromatogram after alignment is executed by a subtraction processor.

A method for determining a quantity of an analyte in a liquid sample, comprises: adding a known quantity of an internal standard comprising an isotopically labeled version of the analyte to the sample; (b) providing a continuous stream of the sample having the internal standard to an inlet of a Liquid Chromatography Mass Spectrometry (LCMS) system; and repeatedly performing the steps of: performing a data-independent analysis of the precursor ion species using a mass analyzer, whereby mass spectra of a plurality of fragment-ion species are acquired; calculating one or more degree-of-matching scores that relate to either a number of ions of the internal standard that overlap between results of the data-independent analysis and tabulated mass spectral data of the internal standard; and performing quantitative tandem mass spectrometric analyses of the internal standard and the analyte if each of the degree-of-matching scores meets a respective degree-of-matching condition.