The present invention discloses a quantitative detection method of multiple metabolic components in a biological sample and a metabolic chip used in the method. The detection method includes performing derivatization treatment on the biological sample and then detecting the derivatized biological sample by liquid chromatography-mass spectrometry. During derivatization treatment, 3-nitrophenylhydrazine is used as a derivatization reagent, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide is used as a derivatization reaction catalyst. According to the detection method of the present invention, high-sensitivity detection can be achieved, multiple metabolic components of different magnitudes can be detected, the operation is simple and fast, and the method is applicable to clinical detection and scientific research examination. The metabolic chip of the present invention includes a chip carrier microtiter plate and related reagents, and quantitative detection of multiple metabolic components of different magnitudes such as amino acid, phenol, phenyl or benzyl derivative, indole, organic acid, fatty acid, sugar, and bile acid in the biological sample on the same microtiter plate can be achieved.

A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance having a property of specifically binding to the target substance; separating the complex and an unbound particle from each other in a liquid by dielectrophoresis, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance included in the separated complex by using an imaging element.

A technique for comprehensively analyzing bile acids, sterols, and hormones is achieved. A method for analyzing bile acids, sterols, and hormones includes: a step of separating a plurality of molecules selected from among bile acids, sterols, and hormones in a sample by reversed-phase liquid chromatography; and a step of ionizing the molecules that have been separated; and a step of detecting, through MS analysis, the molecules that have been ionized.

According to the application, by measuring mass spectra of urine samples and/or serum samples in a healthy group, a negative control group, a positive control group and a treatment group of a drug to be evaluated, the mass spectra of the samples of the treatment group of the drug to be evaluated and the mass spectra of the samples of the negative control group are compared to calculate a difference between peak areas of characteristic fragment particle peaks of metabolic markers in the samples of the treatment group of the drug to be evaluated and in the samples of the negative control group, and a difference value is used to evaluate multidrug resistance of the drug to be evaluated. The larger the difference value is, the larger a callback value is, and the better an effect of reversing the tumor multidrug resistance is.

The present disclosure relates to the use of vapor deposition coated flow paths for improved chromatography and sample analysis using liquid chromatography-mass spectrometry (LC/MS) or liquid chromatography-optical detection (LC/UV). More specifically, this technology relates to separating and quantitation of analytes (e.g., phospho prodrugs and its phosphorylated metabolites) from a sample matrix (e.g., mammalian blood, plasma) using chromatographic devices and fluidic systems having coated flow paths. The LC-MS or LC-UV techniques provide improved recovery, peak shape and dynamic range in the analysis of the prodrug and its metabolites.

One mode of a method for analyzing acylcarnitine according to the present invention is a method for analyzing C5 acylcarnitine using a tandem mass spectrometer, the method including: a measurement step of performing MRM measurement on a specimen according to at least one of MRM transitions of m/z 24>187, m/z 246>57, m/z 246>41, and m/z 246>29; and a processing step of distinguishing between isovalerylcarnitine and pivaloylcarnitine which is an isomer of isovalervicarnitine in the specimen or determining whether or not C5 acylcarnitine in the specimen includes pivaloylcarnitine, using a measurement result obtained in the measurement step. This makes it possible to satisfactorily distinguish between isovalerylcarnitine and pivaloylcarnitine, which have been conventionally difficult to distinguish.

A reader device includes a camera board, processing circuitry in communication with the camera board, and an interface in communication with the processing circuitry. The camera board is configured to capture image data associated with an output signal area of a biological chromatographic test strip. The processing circuitry is configured to determine, based on the image data captured by the camera board, a concentration of a target analyte in a test sample submitted via the biological chromatographic test strip. The interface is configured to output data indicative of the concentration of the target analyte determined by the processing circuitry

The present invention relates to a method for providing a verified analyte measurement of a sample with a chromatography mass spectrometer device, said method comprising the following steps: a) admixing an interferent monitoring compound and, optionally an internal standard, to the sample; b) determining a chromatogram of the sample by acquiring a plurality of data points for signal intensities over time for said interferent monitoring compound, said analyte, and optionally said internal standard; and c) comparing a property of an interferent monitoring compound peak to a property of an internal standard peak and/or to a property of an analyte peak; and to methods and systems related thereto.

A reader device includes a camera board, processing circuitry in communication with the camera board, and an interface in communication with the processing circuitry. The camera board is configured to capture image data associated with an output signal area of a biological chromatographic test strip. The processing circuitry is configured to determine, based on the image data captured by the camera board, a concentration of a target analyte in a test sample submitted via the biological chromatographic test strip. The interface is configured to output data indicative of the concentration of the target analyte determined by the processing circuitry.

A method of processing of a biological sample containing multiple metabolites is described The method comprising the steps of pre-treating the biological sample with a metabolite extraction solvent to provide a pre-treated sample, separating a first aliquot of the pre-treated sample by reverse phase liquid chromatography (RPLC) to provide a first eluent containing resolved hydrophobic metabolites, and separating a second aliquot of the pre-treated sample by hydrophilic interaction liquid interaction chromatography (HILIC) to provide a second eluent containing resolved hydrophilic metabolites. The first and second eluents are assayed using targeted tandem mass spectroscopy operated in multiple reaction monitoring mode. Each liquid chromatography step(LC) is directly hyphenated with the tandem mass spectrometry (MS/MS) into a single LC-MS/MS analysis. The extraction solvent typically comprises methanol, isopropanol and an acetate buffer.