A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

An alignment system, in an example, may include a substrate comprising at least one nanowell, at least one fluid ejection device comprising at least one die, the at least one die comprising as least one nozzle, and an alignment device to align the at least one nozzle to the at least one nanowell.

A method of facilitating controlled particle deposition from a droplet dispenser involves receiving at least one pre-dispensed image of a dispensing portion of the droplet dispenser, the dispensing portion including fluid to be dispensed in a subject droplet to a subject target region, receiving at least one post-dispensed image of the dispensing portion of the droplet dispenser after the subject droplet has been dispensed, comparing the at least one pre-dispensed image and the at least one post-dispensed image to determine a subject droplet particle count representing a count of particles included in the subject droplet, and producing signals for associating a representation of the subject droplet particle count with the subject target region. Other methods, apparatuses, systems, and non-transitory computer readable media are disclosed.

A digital dispense system for preparing and analyzing a plurality of samples. The system includes two or more fluid droplet ejection devices. Each fluid droplet ejection device contains a fluid droplet ejection cartridge containing at least one fluid to be dispensed. The fluid droplet ejection cartridge is attached to a translation mechanism for moving the fluid droplet ejection cartridge back and forth over a sample holder in an x direction. A sample tray translation mechanism is provided for moving a sample tray along a production path in a y direction orthogonal to the x direction through the two or more fluid droplet ejection devices.

Aspects of the present disclosure relate to evaporation compensation in fluidic devices. An example apparatus for evaporation compensation includes an assessment circuit to determine an amount of evaporation of a volume dispensed in a microwell of a fluidic device. The amount of evaporation may be determined based on the volume in the microwell, and an amount of time after dispensing the volume in the microwell. A compensation circuit may determine, based on the amount of evaporation, a compensation factor for the microwell including an amount of a normalizing fluid to compensate for the amount of evaporation. The compensation circuit may also create a normalization profile for the fluidic device, including an association between the fluidic device and the compensation factor. A dispensing circuit may dispense the normalizing fluid in the microwell according to the normalization profile.

A system and method for treatment of biological samples is disclosed. In some embodiments, an automated biological sample staining system (100), comprising at least one microfluidic reagent applicator (118); at least one bulk fluid applicator (116); at least one fluid aspirator; at least one sample substrate holder; at least one relative motion system; and a control system (102) that is programmed to execute at least one staining protocol on a sample mounted on a substrate that is held in the at least one sample substrate holder.

An open fluid droplet ejection cartridge containing one or more fluids to be dispensed by digital fluid dispense system and a method for digitally dispensing fluids. The open fluid droplet ejection cartridge includes a cover having one or more openings therein, fluid funnels pending from each of the one or more openings in the cover, a chamber separator attached to the fluid funnels for directing fluid to one or more fluid chambers, a fluid overflow chamber for fluid overflow from the one or more fluid chambers, fluid vias associated with each of the one or more fluid chambers, and a plurality of fluid ejection devices adjacent to the fluid vias on a single semiconductor substrate in fluid flow communication with the fluid vias.

According to one embodiment, a droplet dispensing apparatus include a droplet ejecting array having a plurality of nozzles from which solution can be ejected into a microplate on a baseplate, a sensor configured to detect a solution amount in the microplate, and a controller configured to detect that a nozzle in the plurality of nozzles is malfunctioning during a solution ejection process based on an initial solution amount in the microplate and a final solution amount in the microplate as detected by the sensor, and control a supplemental droplet dispensing operation in which an additional solution amount is ejected into the microplate based on the initial solution amount and the final solution amount in the microplate.

A system and method for ejecting one or more fluids from a digital dispense device. The method includes selecting a) fixed target areas and total fluid volumes for the target areas of the substrate; b) a predetermined droplet volume for the target areas; c) calculating a required number of droplets for the target areas; d) determining a maximum number of droplets per pixel based on the target areas; e) calculating a number of droplets per pass of an ejection head over the target areas; f) modifying one or more dimension of the target areas to create modified target areas; g) selecting and centering the modified spot size target areas in the target areas; and h) depositing fluids in the modified target areas while scanning the ejection head over the modified target areas.

A digital dispense system and methods for preparing samples for analysis. The digital dispense system includes a fluid droplet ejection system housed in a housing unit. The fluid droplet ejection system contains a fluid droplet ejection head and fluid cartridge containing one or more fluids to be dispensed. A cartridge translation mechanism is provided for moving the fluid droplet ejection head and fluid cartridge back and forth over a sample holder in an x direction. A sample tray translation mechanism moves a sample tray back and forth beneath the fluid droplet ejection head and fluid cartridge in a y direction orthogonal to the x direction.