Aspects of this disclosure relate to systems, devices, and methods for the transfer of fluid to fluidic receptacles. In some embodiments, the fluid contains one or more molecules (e.g., one or more peptides, proteins, and/or nucleic acids) of interest, and the fluidic receptacle includes an integrated device. In certain embodiments, the one or more molecules can be immobilized on the integrated device for subsequent analysis (e.g., sequencing). Certain aspects of the present disclosure are directed towards systems and methods that can, in some instances, enhance the immobilization of the one or more molecules on the integrated device, e.g., by improving a rate of sample interaction with the integrated device. Through the use of systems, devices, and methods of the instant disclosure, target molecules may be more readily sequenced or prepared for sequencing. For example, in some embodiments, systems, devices, and methods of the instant disclosure allow automated loading of the fluidic receptacle using a fluidic device.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.

An automatic analysis device which can be accessed from a front surface of the device to a rear surface side of the device when a user accesses the automatic analysis device and reduce the risk of damage to a rod-shaped member due to contact. A reagent suction position, a reagent discharge position, a reagent dispensing nozzle cleaning portion, and a reagent dispensing mechanism retraction portion from which the reagent dispensing mechanism is retracted are disposed on a trajectory of an arm of the reagent dispensing mechanism. A nozzle guide accommodation portion accommodates a nozzle guide of a reagent dispensing mechanism and the reagent dispensing nozzle. The reagent dispensing mechanism retraction portion is a cylindrical member protruding downward from an upper surface cover of the automatic analysis device and prevents direct contact with the reagent dispensing nozzle by positioning the reagent dispensing nozzle in the nozzle accommodation portion.

A pipette includes a capillary, a pressure chamber, a drive unit, and a control unit. The capillary has a first end and a second end that are two ends in a length direction and that are open. The pressure chamber communicates with an inside of the capillary via the second end. The drive unit changes a volume of the pressure chamber. The control unit controls the drive unit. The control unit outputs a vibrational movement signal that drives the drive unit so that a liquid moves from a mid-position in the capillary to a finish position that is located closer to the second end than the mid-position. The vibrational movement signal has a waveform that drives the drive unit so that the volume of the pressure chamber alternately increases and decreases repeatedly.

In one embodiment, a method is provided comprising analyte testing on one or more types of samples.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alga, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

A system, methods, and apparatus are described to collect and prepare single cells, nuclei, subcellular components, and biomolecules from specimens including tissues. The system can perform enzymatic and/or physical disruption of the tissue to dissociate it into single-cells or nuclei in suspension or subcellular components including nucleic acids. In some embodiments, the titer of dissociated cells is monitored at intervals and the viability determined. In some embodiments, the processing is adjusted according to the measurements of the titer and viability. In some embodiments, the single-cells or nuclei in suspension are washed and resuspended in the buffer or media of choice. In some embodiments, the conditions are chosen to produce nuclei. In other embodiments, the single-cells or nuclei are purified by affinity paramagnetic bead processing. In some embodiments, matched bulk nucleic acid to the single-cells is produced. In other embodiments, single-cell libraries, or nuclei libraries, or matched bulk libraries, or bulk libraries are produced. The single cells or nuclei can then be further processed by FACS, DNA sequencing, mass spectrometry, fluorescence, or other methods. In other embodiments, the tissue processing is integrated with an analytical system to produce a sample-to-answer system such as a tissue-to-genomics system.

The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

Provided are devices for automated analysis of one or more samples in single or multi-well plates or vessels, wherein the process of automated analysis comprises automated flow, wherein the samples comprise liquid or particles in a sample vessel, and wherein the devices comprise an assembly of components that enable processing of a sample for analytical assessment by fluidic and/or particle based instruments. Automated flow may comprise systems for moving samples including vacuum systems, pressure-based systems, pneumatic systems, pumps, peristaltic pumps, diaphragms, or syringes. The devices may comprise an assembly of components that enable movement in X, Y, and Z dimensions, as well as switches, microfluidic tubing, well plate block, electronic pressure controllers, pneumatic or fluidic mixing devices, components for fluid handling, sampling vessels, and mechanical components for translating or transporting system components.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.