Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.

Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.

Provided is a virus like particle comprising one or more zika virus structural proteins, and a composition or vaccine comprising thereof, its use in the prevention or treatment of zika virus disease. The zika virus structural protein contains at least one amino acid alteration in the envelope protein.

The present invention relates to mutant peptides of the E protein of the West Nile virus and other flaviviruses useful for discriminating flaviviral infections, as well as kits, methods and uses related thereto.

Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

A nanobody classical swine fever virus (CSFV)-E0-Nb1 against a CSFV E0 protein, and an encoding gene and use thereof are provided, belonging to the technical field of biological detection. The nanobody CSFV-E0-Nb1 has an amino acid sequence shown in SEQ ID NO: 1 and can be expressed using an expression system. The nanobody is coupled with a quantum dot to obtain an immunochromatographic test strip for distinguishing antibodies against a CSFV E2 subunit vaccine strain from those of a wild strain infected on site, and there is a simple production process of the test strip. The immunochromatographic test strip can differentiate and diagnose the antibodies against the CSFV E2 subunit vaccine strain and the wild strain, and has the advantages of rapid, convenient, and instant detection, thus providing a new method for the detection of classical swine fever (CSF) purification.

Disclosed are a preparation method and application of a nanobody targeting dengue virus NS1 protein. The nanobody targeting dengue virus NS1 protein is a VHH antibody, which is obtained by constructing a nanobody phage library through phage screening technology, and has an amino acid sequence shown in SEQ ID NO: 1. The nanobody in the present disclosure exhibits high affinity and specificity when being bound to the dengue virus NS1 protein, and has advantages of good stability and low cost compared with monoclonal antibodies. Therefore, the nanobody in the present disclosure can be produced on a large scale, and has bright application prospects in the field of dengue virus detection and analysis.

Disclosed are a preparation method and application of a nanobody targeting dengue virus NS1 protein. The nanobody targeting dengue virus NS1 protein is a VHH antibody, which is obtained by constructing a nanobody phage library through phage screening technology, and has an amino acid sequence shown in SEQ ID NO: 1. The nanobody in the present disclosure exhibits high affinity and specificity when being bound to the dengue virus NS1 protein, and has advantages of good stability and low cost compared with monoclonal antibodies. Therefore, the nanobody in the present disclosure can be produced on a large scale, and has bright application prospects in the field of dengue virus detection and analysis.

Provided herein are methods using machine learning to predict protein variants that are likely to occur in nature. Such variants can be used (selected) to improve properties of the proteins. Also provided herein are antibodies and antigen binding portions thereof generated using the provided methods that specifically bind several antigens from coronaviruses, ebolaviruses, and influenza A viruses, various compositions of such antibodies or antigen binding portions thereof, recombinant nucleic acids encoding the antibodies and antigen binding portions thereof, and associated methods of use.

This application relates generally to flavivirus immunogens and to methods and compositions related thereto. More particularly, the disclosure relates to compositions and methods for the preparation, production, and administration of flavivirus immunogens comprising modified E proteins, including, for example, compositions for use as vaccines against flavivirus and for capturing antibodies against flavivirus.