Disclosed are methods of detecting the presence of Loa loa in a biological sample using one or more antigens, each having a different amino acid sequence selected from the group consisting of SEQ ID NOs: 1-20. Related compositions, specific binding partners, and test kits are also disclosed.

The invention relates to a device (1) and a method for determining the action of active ingredients on nematodes and other organisms in aqueous tests. The device (1) according to the invention comprises a holder (13) for a cell culture plate (30) having multiple wells (31) in which the nematodes can be filled with the active ingredients, said cell culture plate (30) having a bottom side (33), a top side (32) and also side walls extending between bottom side (33) and top side (32), a camera (11) which is used to record images of preferably the bottom side (33) of the cell culture plate (30), a lighting mechanism (14) having at least a first light source (15) which illuminates the cell culture plate (30), there being arranged between the first light source (15) and a first side wall (34) of the cell culture plate (30) in the installed state a first optical unit which directs the light of the first light source (15) through the first side wall (34) in the direction of the bottom side (33) of the cell culture plate (30). The method according to the invention makes it possible to simultaneously investigate many active ingredients within a very short time.

The following discloses a novel model for parasitic worm infection, which is useful for screening for and evaluating the efficacy of antiparasitic agents. In particular, the disclosure provides an immunocompromised mouse model that, for the first time, supports advanced development of Dirofilaria immitis (Di) parasites outside of their definitive hosts. As described herein, early-stage larval forms of Di, the causative agent of Canine Heartworm disease, not only persist in this model, but they develop into mature worms.

The present disclosure provides a microfluidic device and system for measuring a composite electropharyngeogram (EPG) signal from a pool of multiple nematodes, wherein the composite EPG signal is measured from the pool of nematodes present in a single recording channel connected to two or more integrated electrodes. The microfluidic device includes an inlet port and outlet port directly connected to a single recording channel and two or more electrodes directly connected to the recording channel. The recording channel is configured to hold 10 to 10,000 nematodes.

The invention relates to a device (1) and a method for determining the action of active ingredients on nematodes and other organisms in aqueous tests. The device (1) according to the invention comprises a holder (13) for a cell culture plate (30) having multiple wells (31) in which the nematodes can be filled with the active ingredients, said cell culture plate (30) having a bottom side (33), a top side (32) and also side walls extending between bottom side (33) and top side (32), a camera (11) which is used to record images of preferably the bottom side (33) of the cell culture plate (30), a lighting mechanism (14) having at least a first light source (15) which illuminates the cell culture plate (30), there being arranged between the first light source (15) and a first side wall (34) of the cell culture plate (30) in the installed state a first optical unit which directs the light of the first light source (15) through the first side wall (34) in the direction of the bottom side (33) of the cell culture plate (30). The method according to the invention makes it possible to simultaneously investigate many active ingredients within a very short time.

An ELISA for the diagnosis of Haemonchus longistipes infection in camels is provided. The ELISA for the diagnosis of Haemonchus longistipes infection in camels includes antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes. The resulting antibodies may be used in assays to detect Haemonchus longistipes infected camels. The assays may be any kind of enzyme-linked immunosorbent assay, or ELISA, known to those of skill in the art. The assay using the antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes infected camels may be capable of detecting pre-patent Haemonchus longistipes infection.

An ELISA for the diagnosis of Haemonchus longistipes infection in camels is provided. The ELISA for the diagnosis of Haemonchus longistipes infection in camels includes antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes. The resulting antibodies may be used in assays to detect Haemonchus longistipes infected camels. The assays may be any kind of enzyme-linked immunosorbent assay, or ELISA, known to those of skill in the art. The assay using the antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes infected camels may be capable of detecting pre-patent Haemonchus longistipes infection.

An ELISA for the diagnosis of Haemonchus longistipes infection in camels is provided. The ELISA for the diagnosis of Haemonchus longistipes infection in camels includes antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes. The resulting antibodies may be used in assays to detect Haemonchus longistipes infected camels. The assays may be any kind of enzyme-linked immunosorbent assay, or ELISA, known to those of skill in the art. The assay using the antibodies raised against a 76 kDa protein isolated from Haemonchus longistipes infected camels may be capable of detecting pre-patent Haemonchus longistipes infection.

The present invention provides compositions and methods that can be used to determine a peptide signature for an antibody repertoire in a sample comprising multiple antibodies. The method can be used to characterize a phenotype in a sample, such as providing a diagnosis, prognosis or theranosis of a medical condition.

Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.