A robotic surgical system includes a hydraulic drive system and a surgical instrument removably positioned in operative engagement with the hydraulic drive system.

The present invention provides compositions that modulate gut microbiota and/or the activity of deoxyribonucleases (e.g., in the gastrointestinal tract) and are useful in the treatment of diseases and disorders related to the activity of deoxyribonucleases including, for example, neurodegenerative, inflammatory, and metabolic diseases and cancer.

Provided herein are methods and compositions for reducing an undesirable T cell immune response in human patients prior to and/or during gene therapy using CRISPR/Cas9-based genetic modulation.

The invention provides methods for treating and diagnosing prostate cancer, as well as related positions and kits.

A method of diagnosing infection in a patient undergoing a treatment of a predetermined type. This method comprises establishing a baseline level of at least one antimicrobial peptide in a specific patient or in a patient population undergoing treatment of a predetermined type, wherein the treatment involves accessing fluid from the patient's peritoneal cavity prior to the patient presenting with a suspected infection; measuring the level of the at least one antimicrobial peptide in a sample taken from the patient after the patient presents with a suspected infection; comparing the baseline level of the at least one antimicrobial peptide to the measured level of the at least one antimicrobial peptide; and diagnosing the patient with an infection if the baseline level of the at least one antimicrobial peptide exceeds the measured level of the at least one microbial peptide by a predetermined amount.

The present invention relates to a method for the analysis of binding and cleavage sites followed by high-throughput sequencing. This method is called “ABC-seq”. The method is based on CUT&RUN (or CUT&Tag), originally developed for the detection of epigenetic marks, in combination with recombinant catalytically active or inactive Cas and a bioinformatics pipeline to identify off-site binding and off-site cleavage events in parallel.

Provided herein are methods amenable to high-throughput multiplexing, in part, using a modified enzyme complementation assay, that can be used to screen a library of test compounds and to identify compounds that inhibit denaturation of a target polypeptide of interest.

A robotic surgical system includes a fluid drive system and a surgical instrument removably positioned in operative engagement with the drive system. A sterile barrier covers non-sterile portions of the surgical system. Features of the sterile barrier are used to transfer motion output from the fluid drive system to the instrument for actuation of the instrument.

The present disclosure provides a library of engineered DNASE proteins (including DNASE1, DNASE1-LIKE 1, DNASE1-LIKE 2, DNASE1-LIKE 3, DNASE2A, DNASE2B) that allows to select drug candidates for developing therapeutics for treating conditions characterized by neutrophil extracellular trap (NET) accumulation and/or release. In accordance with the invention, the selected DNase variant has improved properties, including properties amenable to clinical development, including manufacturing, toxicology, pharmacokinetic, and/or use in therapy.

The present disclosure is directed to a polynucleotide capable of signaling under preselected conditions. For example, the present disclosure relates to a method of reconfiguring a nucleic acid or polynucleotide, including: contacting a deoxyribonucleic acid (DNA) nanoswitch and nucleic acid to form a DNA nanoswitch-nucleic acid complex having a first conformation, wherein the first conformation is characterized as locked; contacting the DNA nanoswitch-nucleic acid complex with a biological specimen to form a mixture, wherein when the nucleic acid is ribonucleic acid (RNA) and the biological specimen includes one or more ribonucleases, the first conformation changes to a second conformation characterized as open; processing the mixture under conditions sufficient to separate the first conformation, and when present, the second conformation; and reacting the first conformation, and when present, the second conformation with an indicator under conditions sufficient to form a signal.