The present invention is related to a one-step formulation formulated for high-throughput analysis, long shelf-life, and stability. The kit includes an enzyme, β-glucuronidase enzyme derived from Brachyspira pilosicoli, in a formulation that meets the physicochemical requirements of reacting quickly and having stability at room temperature. The one-step formulation is intended for use in the detection of foreign substances within a subject's system.

The present disclosure provides method of detecting senescent cells in a cell sample and methods of treating a disease, disorder, or condition associated with senescence in a subject by administering at least one senolytic agent to the subject.

A homogeneous phase detection method. The homogeneous phase detection method comprises the following steps: providing an aptamer and an enzyme, the aptamer being capable of specifically identifying an analyte, connecting the aptamer to the enzyme to produce an aptamer-enzyme compound; configuring analyte standard solutions of different concentrations, adding the aptamer-enzyme compound and an enzyme action substrate to the analyte standard solutions for enzyme-catalyzed reactions, measuring enzyme-catalyzed reaction signals, acquiring a formula for the enzyme-catalyzed reaction signals and the content of the analyte; adding the aptamer-enzyme compound and the substrate into a sample solution containing the analyte for an enzyme-catalyzed reaction, measuring an enzyme-catalyzed reaction signal, and calculating the content of the analyte in the sample solution on the basis of the formula. The homogeneous phase detection method is characterized by high sensitivity, great repeatability, strong anti-interference properties, fast detection rate, and inexpensiveness, allows the detection of various biological molecules, and is very widely applicable.

Described are methods for generating a reporter molecule in response to a target analyte in a cell-free system. A synthetic biological circuit is used to modify the level of the reporter molecule in response to the presence of the target analyte. The reporter molecule may be glucose or another molecule readily detected using a device such as glucose monitor or other portable sensor. Also provided are kits comprising a cell-free system with a synthetic biological circuit that generates or consumes a reporter molecule in response to a target analyte.

There is provided a method of analyzing a biological sample component that allows easy and accurate quantification and counting of any of a plasma component and a blood cell component in a trace and unknown amount of a whole blood sample collected from a finger, for example. The method of the present invention is a method of analyzing a biological sample component in a trace amount of blood, comprising analyzing a diluent buffer into which the blood has been mixed and an internal standard substance and/or an external standard substance contained in the diluent buffer, calculating a dilution ratio, and analyzing a biological component in a plasma or serum component in the blood.

There is provided a method of analyzing a biological sample component that allows easy and accurate quantification and counting of any of a plasma component and a blood cell component in a trace and unknown amount of a whole blood sample collected from a finger, for example. The method of the present invention is a method of analyzing a biological sample component in a trace amount of blood, comprising analyzing a diluent buffer into which the blood has been mixed and an internal standard substance and/or an external standard substance contained in the diluent buffer, calculating a dilution ratio, and analyzing a biological component in a plasma or serum component in the blood.

Provided are methods of assessing activity of a promoter region. The methods include culturing a cell including a nucleic acid, the nucleic acid including a region that encodes an enzyme donor (ED) operably coupled to a promoter region, under conditions in which the ED is expressed when the promoter region is active. The methods further include contacting the ED, if expressed, with an enzyme acceptor (EA) to form ED-EA complexes having enzymatic activity. The methods further include detecting the level of the enzymatic activity to assess activity of the promoter region. Activity of the promoter region may be indicative, and therefore may be used to assess, the activity of a cellular signaling pathway of interest and/or of endogenous or exogenous (e.g., introduced) transcription factors of interest. Cells, compositions, and kits that find use, e.g., in practicing the methods of the present disclosure, are also provided.

There is provided a method of analyzing a biological sample component that allows easy and accurate quantification and counting of any of a plasma component and a blood cell component in a trace and unknown amount of a whole blood sample collected from a finger, for example. The method of the present invention is a method of analyzing a biological sample component in a trace amount of blood, comprising analyzing a diluent buffer into which the blood has been mixed and an internal standard substance and/or an external standard substance contained in the diluent buffer, calculating a dilution ratio, and analyzing a biological component in a plasma or serum component in the blood.

The present invention relates to a symbiotic composition comprising a probiotic bacterial strain and a prebiotic growth medium which is specific to the growth of the probiotic bacterial strain, wherein the bacterial strain is capable of producing the same growth medium by reverse enzyme reaction. The present invention also relates to methods of producing and screening for such compositions.

A kit and method for rapid and cost-effective culturing and/or detecting viruses, bacteriophage and/or bacteria is provided. The viruses, bacteriophage and/or bacteria are cultured and/or detected within, or on, an absorptive fibrous matrix, such as a cellulose fibre absorbent pad, which allows both growth and detection of the viruses, bacteriophage and/or bacteria. Detection may be qualitative, semi-quantitative or quantitative.