A microscope, which is a confocal microscope converted into a light sheet microscope, includes a microscope body and a mechanical receiving apparatus for microscope objectives, through which a microscope beam path extends. An optical module attachable to the receiving apparatus is configured to illuminate a sample volume and collect and transmit light from the sample volume. The optical module comprises: first and second optical arrangements with first and second beam paths that intersect in the sample volume, an optical beam path selector configured to combine the first and/or second beam path with the microscope beam path, and an attachment element arranged between the first or second optical arrangement and the sample volume, wherein the first or second beam path extend at least in sections through the attachment element in order to generate a light sheet. An area sensor is configured to detect light collected from the sample volume.

A slit lamp microscope according to some aspect examples includes an illumination system and photographing system. The illumination system projects slit light onto an anterior segment of a subject's eye. The photographing system includes an optical system and an image sensor. The optical system directs light from the anterior segment onto which the slit light is being projected. The image sensor includes a light detecting surface that receives the light directed by the optical system. Further, a subject plane, a principal plane of the optical system, and the light detecting surface are arranged so as to satisfy a Scheimpflug condition. Here, the subject plane includes a focal point of the illumination system in which a position of the focal point is shifted on account of a refractive index of a tissue of the anterior segment.

The disclosed subject matter includes devices and systems for extending the imaging capability of swept, confocally aligned planar excitation (SCAPE) microscopes to in vivo applications. In embodiments, the SCAPE microscope can be implemented as an endoscopic or laparoscopic inspection instrument.

Provided are an imaging unit 104 that uses a light emitted from a second beam splitter 202 of a microscope 2 that can use an exciting light and an observation light, which is a light including a wavelength other than that of the exciting light, as a light source by switching there between and is provided with the second beam splitter 202 to image images of the same observation region of the microscope 2 in situations where the exciting light and the observation light are used as the light source and an output unit 106 that overlaps, synthesizes, and outputs the images imaged by the imaging unit 104 respectively using the exciting light and the observation light as the light source.

The dynamic energy and spot size adjustment method for laser processing with an optical microscope is applied to a laser processing machine which includes a console, a calculation module, a laser source, a beam adjustment unit, a galvanometer scanner, a light sensor, a vision module, an F-theta lens, a beam splitter, and an objective lens. The laser source generates a laser beam passing through the beam adjustment unit to form a laser processing beam which further passes through the galvanometer scanner, the F-theta lens, the beam splitter, and the objective lens to focus on a working plane. The beam splitter respectively guides parts of the laser processing beam to the vision module and the light sensor. The vision module and the light sensor cooperate with the calculation module to identify/measure and record the energy and spot size of the laser processing beam as dynamic adjustment references during laser processing.

In a method for operating a microscope, a lens unit transmits a first image laser to an object, and acquire a first scan image on the basis of a first reflection signal reflected from a first area included in the object. An ultrasound conversion unit transmits an ultrasound signal to the first area and focuses same so as to form air bubbles in the first area. The lens unit transmits a second image laser to the object, and can acquire a second scan image on the basis of a second reflection signal reflected from the second area included in the object. The ultrasound conversion unit transmits an ultrasound signal to the first area included in the object and focuses same so as to form air bubbles in the first area, thereby enabling an increase in the imageable depth of a microscope.

Provided herein is a macroscope comprising an objective apparatus comprising a multifocal widefield optics comprising a plurality of optical components configured to focus on a plurality of planes. Also provided herein are methods for analyzing a three-dimensional specimen, the method comprising obtaining, via a macroscope, synchronous multifocal optical images of a plurality of planes of the three-dimensional specimen, wherein the macroscope comprises an objective apparatus comprising a multifocal widefield optics comprising a plurality of optical components configured to focus on a plurality of planes. The three-dimensional specimen can be a biological specimen, such as brain.

Methods and apparatus for obtaining 3D imaging of tissue involve scanning a focused laser beam in xz planes to obtain a set of xz plane images spaced apart in a y direction. The xz plane images are processed to correct distortions and motions and combined to provide 3D image data. Surface flatting is optionally performed. Imaging may be performed using a femtosecond (fs) laser beam. Different components of light returning from the tissue may be detected and processed to yield plural co-registered images using different imaging modalities, for example, reflective confocal microscopy (RCM), two photon fluorescence (TPF) and second harmonic generation (SHG).

An apparatus for measuring a surface topography of a patient's teeth may include an optical probe, a light source configured to generate incident light, and focusing optics configured to focus one or more wavelengths of the incident light to a fixed focal position external to the optical probe, wherein the fixed focal position is fixed relative to the optical probe. The apparatus may further include a light sensor configured to measure a characteristic of returned light generated by illuminating the patient's teeth with the incident light and a processing unit operable to determine the surface topography of the patient's teeth based on the measured characteristic of the returned light.

Disclosed are an endoscopic reflection microscope using an optical fiber bundle and an image acquisition method using the same. The endoscopic reflection microscope includes an incident wave output unit configured to output an incident wave to a target object through any one optical fiber in an optical fiber bundle, a reflected wave receiver configured to receive a reflected wave output from the target object in response to the incident wave through a plurality of corresponding optical fibers in the optical fiber bundle, and an image acquirer configured to establish a reflection matrix corresponding to the reflected wave and to acquire an image in which at least one of phase retardation of the incident wave or phase retardation of the reflected wave is compensated for based on the established reflection matrix.