A method for adjusting a focus of an optical system includes focusing measurement light in a sample space using an optical arrangement. The measurement light is transmitted on a sample side of the optical arrangement through at least one optical medium. The measurement light reflected by a reflector and transmitted through a further optical arrangement is detected using a detector arrangement. A working distance between the optical arrangement and the reflector is ascertained based on the measurement light detected by the detector, wherein a focus of the measurement light lies on the reflector for the working distance.

Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

A lighting device for an imaging optical device such as a microscope is provided. The lighting device illuminates an object to be analyzed in an imaging optical device for microscopic analysis in at least two different contrasting techniques. The lighting device has light sources for the illumination, where the light sources are associated with a contrasting technique are controllable independently from each other.

A delivery fiber assembly suitable for delivering broad band light and including a delivery fiber and a connector member. The delivery fiber has a length, an input end for launching light, and a delivery end. The delivery fiber includes along its length a core region and a cladding region surrounding the core region, the cladding region includes a cladding background material having a refractive index N.sub.bg and a plurality of microstructures in the form of inclusions of solid material having refractive index up to N.sub.inc and extending in the length of the longitudinal axis of the delivery fiber, wherein N.sub.inc<N.sub.bg. The plurality of inclusions in the cladding region is arranged in a cross-sectional pattern including at least two rings of inclusions surrounding the core region. The connector member is mounted to the delivery fiber at a delivery end section of the delivery fiber including the delivery end.

Improved fluoresced imaging (FI) endoscope devices and systems are provided to enhance use of endoscopes with FI and visible light capabilities. An endoscope device is provided for endoscopy imaging in a white light and a fluoresced light mode. A chromatic adjustment assembly, typically implemented with prisms, compensates for a chromatic focal difference between the white light image and the fluoresced light image caused by the dispersive properties of the optical materials or optical design employed in the construction of the optical channel. The assembly is placed optically between the most proximal rod lens of the endoscope and the focusing optics, typically at an internal telecentric image space, to improve the chromatic correction. The prism assembly directs incoming light with different spectral content along separate paths which compensate for chromatic aberration.

A light source unit includes: a housing; a semiconductor laser that is disposed in the housing and that radiates excitation light; a first condenser optical system that condenses the excitation light; a dichroic mirror that selectively reflects the excitation light; a second condenser optical system that condenses the excitation light; a wavelength conversion member that performs wavelength conversion of the excitation light and emits wavelength-converted light; an emission section that outputs the wavelength-converted light transmitted through the second condenser optical system and the dichroic mirror; and a light blocking section that is disposed between an inner surface of the housing, the inner surface being in a traveling direction of the excitation light toward a reflection surface of the dichroic mirror, and a back surface, the back surface being an opposite side of the reflection surface, or is disposed on the inner surface of the housing.

Methods and systems are provided for improving resolution, acquisition speed, and/or illumination dose for microscopy systems. In some embodiments, a microscopy system having multiple objective setups may include illumination generators to provide selectively-blanked illumination line scans, objective lenses to introduce the selectively-blanked illumination line scans to a sample and to collect fluorescence emissions from the sample, and detectors to receive the fluorescence emissions from the objective lenses. The microscopy system may also include one or more processors in operative communication with the detectors, which may combine the fluorescence emissions to generate a composite image.

The technology disclosed present systems and methods to produce an enhanced resolution image from images of a target using structured illumination microscopy (SIM). The method includes transforming at least three images of the target captured by a sensor in a spatial domain into a Fourier domain to produce at least three frequency domain matrices that each include first blocks of complex coefficients and redundant second blocks of complex coefficients that are conjugates to the first blocks. The method includes reducing computing resources required to produce the enhanced resolution image by using first blocks of complex coefficients to produce at least three phase-separated half-matrices in the Fourier domain. The method includes performing one or more intermediate transformation on the phase-separated half-matrices to produce realigned shifted half-matrices. The method includes calculating complex coefficients of second blocks in the Fourier domain to produce full matrices from half-matrices.

Provided are a device for analyzing a large-area sample based on an image, a device for analyzing a sample based on an image by using a difference in medium characteristic, and a method for measuring and analyzing a sample by using the same. The device for analyzing a large-area sample includes a first sensor array including sensors disposed while being spaced apart from each other in a first direction, a second sensor array including sensors disposed while being spaced apart from each other in the first direction, and spaced apart from the first sensor array in a second direction, and a control unit that obtains image data for a cell included in the sample by using sensing data of the sensor on the sample, in which the sample is interposed between the first sensor array and the second sensor array.

A computer-implemented method for determining a value for at least three setting parameters by means of an input unit in the form of a graphical user interface with an input cursor positionable in an input area is provided. At least two of the at least three setting parameters can be set independently of one another. The method includes determining a position of the input cursor within the input area; determining a coordinate of the position of the input cursor for each particular setting parameter of the at least three setting parameters as a function of a distance in each case of the position of the input cursor from at least one coordinate origin allocated to the particular setting parameter; and determining a value for each particular setting parameter of the at least three setting parameters as a function of the coordinate determined for each particular setting parameter.