Embodiments are for a confocal microscope unit attached to a connection port of a microscope including: light sources which output irradiation light to a sample to be observed; photodetectors which detect observation light generated from a sample in response to the irradiation light; a scan mirror which scans the irradiation light on the sample and guides the observation light generated from the sample to the photodetectors; a scan lens which guides the irradiation light scanned by the scan mirror to the microscope optical system and guides the observation light focused by the microscope optical system to the scan mirror; a lens barrel to which the scan lens is fixed; an attachment portion which attaches the lens barrel to the connection port; and a movable portion which supports the lens barrel so that an angle of the lens barrel with respect to the attachment portion is changeable.

A method is used to generate an analysis image of a moving sample based on one or more exposures. An illumination source illuminates a field of view of a camera for one or more pulses while the sample moves through the field of view. The distance moved by the sample during each of these one or more pulses may be less than the size of one pixel in an image captured by the camera.

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

Systems and methods for managing a plurality of scanning devices in a high-throughput laboratory environment. Each of the scanning devices is configured for a remote boot operation from an administrative server that is communicatively coupled with the plurality of scanning devices via a local network. The remote boot replaces the complete operational firmware of a scanning device. The scanning devices are each configured to periodically provide operational information to the administrative server for centralized storage. The centralized storage of operational information for each of the plurality of scanning devices, coupled with the ability of the administrative server to initiate a reboot of any scanning device and thereby update the complete operational firmware of the scanning device, allows for centralized administration of multiple scanning devices that facilitates configuration, support, image data storage, and/or communication with outside servers.

An optical imaging equipment and method. The optical imaging equipment includes an optical microscope, an objective table, a light source module and an objective lens. The objective table is movable in the XY-plane, the light source module contains illumination light sources, a narrowband filters and the objective lens is movable in the Z-axis direction; a three dimensions (3D) electric sample table is fixed on the objective table, which is used for carrying a sample to be tested and driving the sample to move in 3D directions relative to the objective table; a microsphere is fixed on a transparent substrate; the objective lens, the microsphere and the sample to be tested are arranged in the Z-axis direction in sequence, wherein, the transparent substrate along with the microsphere thereon can be moved to a first position and remain stationary relative to the objective table in the Z-axis direction, the 3D electric sample table can adjust the sample to be tested with respect to the microsphere to an imaging plane which is parallel to the XY-plane and a first image is formed by the microsphere, the objective lens can be adjusted to a second position so that the objective lens can perform a secondary imaging of the first image to form a second image.

A microscope, which is a confocal microscope converted into a light sheet microscope, includes a microscope body and a mechanical receiving apparatus for microscope objectives, through which a microscope beam path extends. An optical module attachable to the receiving apparatus is configured to illuminate a sample volume and collect and transmit light from the sample volume. The optical module comprises: first and second optical arrangements with first and second beam paths that intersect in the sample volume, an optical beam path selector configured to combine the first and/or second beam path with the microscope beam path, and an attachment element arranged between the first or second optical arrangement and the sample volume, wherein the first or second beam path extend at least in sections through the attachment element in order to generate a light sheet. An area sensor is configured to detect light collected from the sample volume.

An analyzer of a component in a sample fluid includes an optical source and an optical detector defining a beam path of a beam, wherein the optical source emits the beam and the optical detector measures the beam after partial absorption by the sample fluid, a fluid flow cell disposed on the beam path defining an interrogation region in the a fluid flow cell in which the optical beam interacts with the sample fluid and a reference fluid; and wherein the sample fluid and the reference fluid are in laminar flow, and a scanning system that scans the beam relative to the laminar flow within the fluid flow cell, wherein the scanning system scans the beam relative to both the sample fluid and the reference fluid.

The invention relates to a method for localizing an emitter (F) in a sample (S) comprising illuminating the sample (S) with a stationary donut-shaped excitation beam (E), acquiring fluorescence photons; and estimating a position of the emitter (F) in the sample (S) from the acquired fluorescence photons. The invention further relates to an apparatus (1) for localizing an emitter (F) in a sample (S) comprising illumination means (10), acquisition means (20) and processing means (30) and a computer program comprising instruction to cause the apparatus (1) to execute the method for localizing an emitter (F).

A method for obtaining image data of a subject, including; a first scanning step including a plurality of steps W, each step W being a step of determining one place existing in a one-dimensional, two-dimensional, or three-dimensional first space; and a second scanning step of scanning insides of second spaces including at least one of the places, wherein the second scanning step includes a step X of randomly determining a location of an observation point and a step Y of obtaining a piece of image data for the observation point, and, at a time point of end of scanning an inside of one of the second spaces, the second space has a first region including 50% of observation points and a second region existing outside the first region and including remaining 50% of the observation points, the second region being larger than the first region by at least 15%.

The present disclosure provides a rapid three-dimensional imaging system based on a multi-angle 4Pi microscope. The system includes: an illumination module, configured to obtain a parallel light of which a size covering a projection surface of a spatial light modulator; a wavefront modulation module, configured to place the LCOS device on a Fourier plane of an illumination end; a two-dimensional scanning module, configured to control a light beam to realize a two-dimensional scanning on an object plane; an illumination interference module, configured to generate point spread function PSFs of a 4Pi through an illumination interference to irradiate a fluorescent sample; an imaging module, configured to acquire interference images of two fluorescent signals; and a controller, configured to control the wavefront modulation module to adjust a polarization direction of the light to generate PSFs of the 4Pi with different inclination angles.