A system having a macroscopic imager, a microscopic imager, and a stage for moving a substrate supporting ex-vivo tissue with respect to each of the imagers to enable the macroscopic imager to capture macroscopic images, and the microscopic imager to capture optically formed sectional microscopic images on or within the tissue, when presented to the tissue, via the optically transparent material of the substrate. A computer system controls movement of the stage, and receives the macroscopic and microscopic images. A display is provided for displaying the macroscopic and microscopic images when received by the computer system. The tissue is verified as being in an orientation at least substantially flush against the upper surface of the substrate by being in focus in displayed macroscopic images prior to imaging by the microscopic imager, and if needed, any portion of the tissue unfocused is manually positioned until desired tissue orientation is achieved.

A laser microscope 1 includes: a filter unit 18, which is a fluorescence-splitting mechanism that splits the fluorescence generated by the specimen S and the excitation light according to a wavelength, and that changes a wavelength at which light is split; a diffraction grating 22 that disperses the fluorescence split by the filter unit 18; a mirror 23 that changes a wavelength of fluorescence that is detected by a PMT 26 and that is dispersed by the diffraction grating 22; and a control unit 30 that controls the filter unit 18. The control unit 30 performs control to change a wavelength at which the filter unit 18 splits light in accordance with a change in the wavelength of the fluorescence that is detected by the PMT 26 and that is dispersed by the diffraction grating 22, the change being performed by the mirror 23.

The present invention relates to digital pathology. In order provide enhanced use of available imaging radiation, a digital pathology scanner (10) is provided that comprises a radiation arrangement (12), a sample receiving device (14), an optics arrangement (16), and a sensor unit (18). The radiation arrangement comprises a source (20) that provides electromagnetic radiation (22) for radiating a sample received by the sample receiving device. Further, the optics arrangement comprises at least one of the group of a lens (24) and a filter (26) that are arranged between the sample receiving device and the sensor unit. The sensor unit is configured to provide image data of the radiated sample. Still further, a lens array arrangement (28) is provided that comprises at least one lens array (30) arranged between the source and the sample receiving device. The at least one lens array comprises a plurality of linear cylindrical lenses (32) that modulate the electromagnetic radiation from the source such that, in an object plane, a radiation distribution pattern (34) is generated with a plurality of first parts of intensified radiation and a plurality of second parts of weak radiation.

Methods of acquiring a through-focus scanning optical microscopy (TSOM) image and inspecting a semiconductor device are provided. A method of acquiring the TSOM image includes: acquiring a plurality of actual images of different focal positions and out-of-focus degrees (distances) of the actual images with respect to an inspection object through an optical tool; acquiring a plurality of virtual images having different focal positions from the actual images and the focal positions thereof, based on the actual images and the out-of-focus degrees of the actual images; and acquiring a TSOM image of the inspection object by using the actual images and the virtual images. According to a method of acquiring the TSOM image and the method of inspecting the semiconductor device, it is possible to acquire high-precision TSOM images of the object with less effort and time and to inspect the semiconductor device efficiently and at low cost.

The invention relates to a device and a method for imaging a sample (2) arranged in an object plane (1). Such a device comprises an optical relay system (3) which images an area of the sample (2) from the object plane (1) into an intermediate image plane (4). Here, the object plane (1) and the intermediate image plane (4) with an optical axis (5) of the relay system (3) include an angle different from 90°. The optical relay system (3) is composed of several lenses. The device also comprises an optical imaging system (6) with an objective, the optical axis (7) of which lies perpendicularly on the intermediate image plane (4) and which is focused on the intermediate image plane (4), with the result that the object plane (1) can be imaged undistorted onto a detector (8). Finally, the device also comprises an illumination apparatus (10) for illuminating the sample (2) with a light sheet (11), wherein the light sheet (11) lies essentially in the object plane (1) and defines an illumination direction, and wherein the normal of the object plane (1) defines a detection direction.

In such a device, the object plane (1) with the optical axis (5) of the relay system (3) includes an angle, the value of which is smaller than the aperture angle of an object-side detection aperture cone (12) of the relay system (3), and the object plane (1) lies at least partially within the object-side detection aperture cone (12). The intermediate image plane (4) with the optical axis (5) of the relay system (3) also includes an angle, the value of which is smaller than the aperture angle of an intermediate image-side detection aperture cone (13) of the relay system (3), and the intermediate image plane (4) lies at least partially within the intermediate image-side detection aperture cone (13).

Implementing swept, confocally aligned planar excitation (SCAPE) imaging with asymmetric magnification in the detection arm provides a number of significant advantages. In some preferred embodiments, the asymmetric magnification is achieved using cylindrical lenses in the detection arm that are oriented to increase the magnification of the intermediate image in the width direction but not in the depth direction. SCAPE imaging may also be improved by using an SLM to modify a characteristic of the sheet of excitation light that is projected into the sample. Additional embodiments include a customized version of SCAPE that is optimized for imaging the retina at the back of an eyeball in living subjects.

There is provided a measurement apparatus including a control unit configured to control an on/off state of illumination that does not contribute to acquisition of measurement data on the basis of an acquisition time period of the measurement data.

A solid immersion lens holder includes a first member having a first opening disposing a spherical face portion therein so that a part of the spherical face portion protrudes toward an objective lens side and a second member having a second opening disposing a contact portion therein so that a contact face protrudes toward a side opposite to the objective lens side. The first member includes three protrusion portions extending from an inner face of the first opening toward a center of the first opening and configured to be contactable with the spherical face portion.

A medical imaging system for illuminating tissue samples using three-dimensional structured illumination microscopy is port-based surgery is provided. The system comprises: an image sensor; a mirror device; zoom optics; a light modulator; a processor; and collimating optics configured to convey one or more images from the modulator to the mirror, the mirror configured to convey the images to the zoom optics, the zoom optics configured: to convey the image(s) from the mirror to a tissue sample; and convey one or more resulting images, formed by the image(s) illuminating the sample, back to the mirror, which conveys the resulting image(s) from the zoom optics to the image sensor, and, the processor configured to control the modulator to form the image(s), the image(s) including at least one pattern selected to interact with the sample to generate different depth information in each of resulting image(s).

This disclosure provides systems and methods for mapping and/or measuring a mechanical property of a medium. The mechanical property can be measured by Brillouin spectroscopy. The systems and methods can include a three-dimensional imaging modality that is co-registered with a Brillouin probe beam of a Brillouin spectrometer. The three-dimensional imaging modality can be optical coherence tomography or Scheimpflug camera imaging.