A microscope system includes: a stage on which a specimen is mounted; one or more light sources configured to emit light irradiating the specimen; an illumination optical system configured to irradiate the specimen with the light; an operating unit configured to receive selection of the light sources and setting of the state and/or an amount of light; a focusing unit configured to move in a direction orthogonal to a mounting surface and adjust the distance between the stage and an objective lens; an imaging unit configured to image the observation image of the specimen and generate image data; and a combined image generating unit configured to combine the image data and generate combined image data. The microscope system enables selection of the state of optical elements constituting the illumination optical system and selection of the type, the state, and an amount of light of the light source.

The present disclosure relates to Fourier ptychographic microscopy systems and methods. The system includes: a three-color LED array; a microscope, configured to obtain image information with multi-wavelength, and magnify to generate magnified image information; an RGB camera configured to acquire a first color image with a first resolution based on the magnified image information; and a controller, configured to synchronously control the three-color LED array and the RGB camera, in which the three-color LED array is further configured to display a plurality of illumination patterns, the RGB camera is further configured to acquire synchronously a plurality of first color images, and the controller is further configured to restore a single second image with a second resolution according to the plurality of first color images, and the first resolution is less than the second resolution. The present disclosure improves sampling speed.

The invention relates to a transmitted light illumination apparatus (2) for a microscope (1), said transmitted light illumination apparatus (2) comprising, a planar light source (20), a mirror (23) having a concave mirror surface (24) arranged in the direction of light emitted from the planar light source (20), and at least one diaphragm element (22) being at least partially opaque and being arranged between the planar light source (20) and the concave mirror surface (24) such that by moving the at least one diaphragm element (22) in at least one direction parallel to a plane defined by the planar light source (20), the planar light source (20) is at least partially covered by the at least one diaphragm element (22).

Certain aspects pertain to Fourier ptychographic imaging systems, devices, and methods such as, for example, high NA Fourier ptychographic imaging systems and reflective-mode NA Fourier ptychographic imaging systems.

A compact microscope including an enclosure, a support element, a primary optical support element located within the enclosure and supported by the support element, at least one vibration isolating mount between the support element and the primary optical support element, a sample stage supported on the primary optical support element to support a sample, a return optical system to receive returned light from a sample and transmit returned light to a detection apparatus, wherein the return optical system is mounted on the primary optical support element, and wherein the compact microscope include a at least one of the following elements; a) an objective lens system, b) a temperature-control system, and c) the return optical system being operable to separate returned light into at least a first wavelength band and a second wavelength band.

A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination.

The invention relates to a system (20) for full-field interference microscopy imaging of a three-dimensional diffusing sample (206). Said system includes: an interference device (200) including a reference arm on which a reflective surface (205) is arranged, the interference device being suitable for producing, at each point of an imaging field when the sample is placed on a target arm of the interference device, interference between a reference wave, obtained by reflection of incident light waves onto a basic surface of the reflective surface (205) corresponding to said point of the imaging field, and a target wave obtained by backscattering of incident light waves by means of a voxel of a slice of the sample at a given depth, said voxel corresponding to said point of the imaging field; an acquisition device (208) suitable for acquiring, at a fixed path length difference between the target arm and the reference arm, a temporal series of N two-dimensional interferometric signals resulting from the interference produced at each point of the imaging field; and a processing unit (220) configured to calculate an image (IB, IC) representing temporal variations in intensity between said N two-dimensional interferometric signals.

The invention relates to a method, a microscope (1) and a computer program for localizing and/or imaging light-emitting molecules (M) in a sample(S) contained in a sample reservoir (6) comprising illuminating the sample(S) by a light source (2) comprised in or connected to a microscope (100), detecting light emitted by the light-emitting molecules (M) in the sample(S) by a detector (3) comprised in or connected to the microscope (100), wherein the light-emitting molecules (M) comprise a bright state (B) and a dark state (D), determining, based on the detected light (F), a current value of one or more parameters, wherein at least one or the parameters co-depends on the photo-physical or photo-chemical properties of the light-emitting molecules (M) other than their average photon emission rate, and adjusting a composition of a fluid in the sample reservoir (6) to optimize the one or more parameters during the localization or imaging of the light-emitting molecules.

Apparatuses and methods are described for detecting inclusions in glass. The apparatuses and methods employ a laser that is configured to project a laser sheet at a first angle from one side of a glass sheet, and a camera configured to capture images from a second angle from another side of the glass sheet. The glass sheet is moved thorough the laser sheet while the camera captures images. One or more processing devices execute image processing algorithms to identify areas of the glass sheet containing inclusions based on the captured images. In some examples, the identified areas of the glass sheet are revisited to confirm they contain inclusions.

A method and system are provided for illuminating and imaging a biological sample using a brightfield microscope for the purpose of counting biological cells. The method comprises positioning a sample to be viewed by way of an objective lens of the microscope, the sample comprising a plurality of biological cells; capturing and storing, using an image capturing apparatus, one or more focal image stacks; processing the one or more focal image stacks using a cell localisation neural network, the cell localisation neural network outputting a list of one or more cell locations; determining, using the list of cell locations, one or more cell focal image stacks, each cell focal image stack being obtained from the one or more focal image stacks; processing the one or more cell focal image stacks using an encoder neural network; determining, using the list of cell locations and the list of cell fingerprints, a number of cells within the sample. The present disclosure aims to provide a quick, non-invasive and reliable mode of counting biological cells.