An example fluid collection assembly includes a fluid impermeable barrier at least defining a chamber, at least one opening, and a fluid outlet. The fluid collection assembly further includes at least one porous material disposed in the chamber and a tube in fluid communication with the fluid outlet, the tube including a hydrophilic material exhibiting a porous structure configured to wick fluid from the chamber without the assistance of a pump.

A dermal patch device for acquisition and analysis of a physiological sample from a subject can include, a lancet port configured to receive a lancet to pierce the skin of the subject and thereby obtain the physiological sample, a sensor disposed in a sensor chamber, and a plurality of fluid pathways. The dermal patch device can further include a first fluid-filled ampoule, a second fluid-filled ampoule, and a third fluid-filled ampoule. The first fluid-filled ampoule can be actuated to release calibration fluid into the sensor chamber via a first group of the fluid pathways. The second fluid-filled ampoule can be actuated to evacuate the calibration fluid from the sensor chamber via a second group of the fluid pathways among the plurality of fluid pathways. The third fluid-filled ampoule can be actuated to draw the physiological sample into the sensor chamber via a third group of the fluid pathways.

The present disclosure relates to the technical field of tests, and in particular, to a thin test strip, a test strip roll, and an application of the thin test strip. The thin test strip includes: a waterproof top layer, a composite layer, and a waterproof bottom layer in sequence from top to bottom, where the composite layer includes a sample collection pad, a colloidal gold conjugate pad, a color developing membrane, and a sample absorption pad, the waterproof top layer does not cover the sample collection pad and completely covers the colloidal gold conjugate pad, the color developing membrane, and the sample absorption pad, and the composite layer is I-shaped. The waterproof top layer and the waterproof bottom layer are made from polyethylene terephthalate (PET) waterproof transparent membranes.