An imaging system includes an infrared illuminator, an ultrasonic emitter, a reference wavefront generator, and an image pixel array. The infrared illuminator emits a general illumination emission into a three-dimensional diffuse medium, where a portion of the general illumination emission encounters a voxel within the diffuse medium. The ultrasonic emitter focuses an ultrasonic signal to the voxel to wavelength-shift the portion of the general illumination emission to generate a shifted infrared imaging signal. The reference wavefront generator generates an infrared reference wavefront having a same wavelength as the shifted infrared imaging signal. The image pixel array captures an infrared image of an interference between the shifted infrared imaging signal and the infrared reference wavefront.

Microscope (2) comprising a coherent light source (4) producing a coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14) configured to split the coherent light beam (7) into a reference beam (7a) and a sample illumination beam (7b), a sample holder (18) configured to hold a sample (1) to be observed, a sample illumination device (28) configured to direct the sample illumination beam (7b) through the sample and into a microscope objective (37), a beam reuniter (16) configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system (8) configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.

A human body security check system based on millimeter wave holographic three-dimensional imaging, comprising a mechanical scanning mechanism, millimeter wave signal transceiver units, an image processing unit (7), and an alarm unit (9). The mechanical scanning mechanism is used for driving the millimeter wave signal transceiver units to simultaneously move horizontally and vertically relative to an individual to be checked (10); the millimeter wave signal transceiver units are used for transmitting millimeter wave signals to the individual to be checked (10) and receiving millimeter wave signals reflected by the individual to be checked (10); the image processing unit (7) is used for performing holographic three-dimensional imaging on the body of the individual to be checked (10) according to the reflected millimeter wave signals so as to obtain a three-dimensional image of the body; the alarm unit (9) is used for comparing the three-dimensional image of the body with a three-dimensional image of a secure body pre-stored in the alarm unit (9), and giving an alarm if the three-dimensional image of the body does not match the three-dimensional image of the secure body pre-stored in the alarm unit. The human body security check system is low in costs because electrical scanning is replaced with mechanical scanning, and features a simple structure, a short production period, high resolution, a short imaging time, and wide application. Also provided is a human body security check method based on millimeter wave holographic three-dimensional imaging.

Systems and methods for simultaneous multi-channel off-axis holography are described. Multi-channel imaging systems can include a light system including a plurality of light sources configured to generate illumination and reference beams at a plurality of wavelengths, an illumination system configured to illuminate a target object with the illumination beams, an optical assembly configured to receive a reflected target beam and condition the target beam for recording at an optical imaging system, and a reference system configured to propagate the reference beams to the optical imaging system. The reference beams are interfered with the target beam at the optical imaging system to create interference patterns, which can be recorded in a collective image having a plurality of side lobes. Holographic information in the side lobes can be combined to generate 3D images having a substantially reduced signal to noise ratio.

A holographic interferometer, comprising: at least one imaging device capturing an interference pattern created by at least two light beams; and at least one aperture located in an optical path of at least one light beam of the at least two light beams; wherein the at least one aperture is located away from an axis of the at least one light beam, thus transmitting a subset of the at least one light beam collected at an angle range.

An infrared image is captured while an infrared reference wavefront and an infrared imaging signal are incident on an image pixel array. A frequency domain infrared image is generated by performing a transform operation on the infrared image. A filtered frequency domain infrared image is generated by applying a mask to the frequency domain infrared image to isolate a frequency representing the interference between the infrared reference beam and the incoming infrared image signal. Intensity data is generated from the filtered frequency domain infrared image. The intensity data is incorporated as a voxel value in a composite image.

According to one embodiment, a beam splitter splits light into first light and second light. The second light is used to irradiate a sample containing particles. A first imaging device images a first interference pattern formed by multiplexing third light, which has been generated by irradiating the particles with the second light, and the first light. A second imaging device images a second interference pattern formed by the third light. An arithmetic device compares a composite image with a calculated image. The composite image is created by using a first interference image picked up by the first imaging device and a second interference image picked up by the second imaging device. The calculated image is obtained by combining single particle interference images, each of which is expected to be obtained by the first imaging device in a case where a particle is present alone in the sample.

Systems and methods for simultaneous multi-channel off-axis holography are described. Multi-channel imaging systems can include a light system including a plurality of light sources configured to generate illumination and reference beams at a plurality of wavelengths, an illumination system configured to illuminate a target object with the illumination beams, an optical assembly configured to receive a reflected target beam and condition the target beam for recording at an optical imaging system, and a reference system configured to propagate the reference beams to the optical imaging system. The reference beams are interfered with the target beam at the optical imaging system to create interference patterns, which can be recorded in a collective image having a plurality of side lobes. Holographic information in the side lobes can be combined to generate 3D images having a substantially reduced signal to noise ratio.

The present invention relates to a full-field reflection phase microscope. In a preferred embodiment, the invention can combine low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. A preferred embodiment of the system uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. With improved path-length sensitivity, the present invention is suitable for full-field measurement of membrane dynamics in live cells with sub-nanometer-scale sensitivity.

An infrared image is captured while an infrared reference wavefront and an infrared imaging signal are incident on an image pixel array. A frequency domain infrared image is generated by performing a transform operation on the infrared image. A filtered frequency domain infrared image is generated by applying a mask to the frequency domain infrared image to isolate a frequency representing the interference between the infrared reference beam and the incoming infrared image signal. Intensity data is generated from the filtered frequency domain infrared image. The intensity data is incorporated as a voxel value in a composite image.