A projection system includes: an illumination source configured to output illumination light; a phase light modulator (PLM) optically coupled to the illumination source, the PLM configured to: receive the illumination light; phase modulate the illumination light while displaying a phase hologram, to produce modulated light; and projection optics coupled to the PLM, the projection optics configured to receive the modulated light and to project an image responsive to the modulated light; wherein both a mean in intensity and a variance in intensity in bright regions of the projected image is greater than the mean intensity and the variance in intensity in dark regions of the projected image.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A phase modulation data generating unit according to the present disclosure includes: a first calculating section; and a storage section. The first calculating section calculates basic phase modulation pattern data on the basis of a partial illumination image pattern that makes it possible to generate an intended illumination image pattern having a desired luminance distribution. The basic phase modulation pattern data makes it possible for a light phase modulation device to reconstruct the partial illumination image pattern. The storage section stores the basic phase modulation pattern data calculated by the first calculating section.

A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

To obtain a more accurate image by improving a utilization efficiency of light energy while at the same time suppressing with a simpler method distortion that may occur in an inline hologram when a plurality of lights having different wavelengths are used, an observation device (1) according to the present disclosure includes a light source part (11) in which a plurality of light emitting diodes (101) having different light emission wavelengths with a length of each light emission point being smaller than 100λ (λ: light emission wavelength) are arranged such that a separation distance between the adjacent light emitting diodes is equal to or smaller than 100λ (λ: light emission wavelength); and an image sensor (13) installed so as to be opposed to the light source part with respect to an observation target object.

Apparatuses and methods for improved reconstructions of electron-based holograms are disclosed herein. An example method at least includes forming a hologram of a sample and a known object, forming a reconstruction of the known object using a reconstruction algorithm, comparing the reconstruction of the known object to a reference reconstruction of the known object, and adjusting the reconstruction algorithm based on the comparison of the reconstruction of the known object to the reference reconstruction of the known object. The example method may further include forming a reconstruction of the sample using the adjusted reconstruction algorithm.

A method of calculating a hologram having an amplitude and a phase component. The method comprises (i) receiving an input image comprising a plurality of data values representing amplitude. The method then comprises (ii) assigning a random phase value to each data value of the plurality of data values to form a complex data set. The method then comprises (iii) performing an inverse Fourier transform of the complex data set. The method then comprises (iv) constraining each complex data value (X1, X2) of the transformed complex data set to one of a plurality of allowable complex data values (GL1-GL8), each comprising an amplitude modulation value and a phase modulation value, to form a hologram, wherein, the phase modulation values (GL1-GL7) of the plurality of allowable complex data values substantially span at least 3π/2 and at least one of the allowable complex data values has an amplitude modulation value of substantially zero (GL8) and a phase modulation value of substantially zero.

A data generating device sets an initial candidate solution of an intensity spectrum function, a phase spectrum function, and an initial temperature and a cooling rate, generates a neighborhood solution, transforms a first waveform function of a frequency domain including the neighborhood solution and the phase spectrum function into a second waveform function of a time domain including a time-intensity waveform function and a time-phase waveform function and calculates an evaluation value representing a degree of difference between the time-intensity waveform function and the desired time-intensity waveform, sets the neighborhood solution as an n-th candidate solution for a certain probability, and lowers the temperature on the basis of the cooling rate. A decrease in the temperature acts in a direction in which the probability P is lowered when the evaluation value of the neighborhood solution is worse than the evaluation value of the candidate solution.

Dual or multi-modulation display systems are disclosed that comprise projector systems with at least one modulator that may employ non-mechanical beam steering modulation. Many embodiments disclosed herein employ a non-mechanical beam steering and/or polarizer to provide for a highlights modulator.

There is provided a holographic projector comprising a hologram engine and a controller. The hologram engine is arranged to provide a hologram comprising a plurality of hologram pixels. Each hologram pixel has a respective hologram pixel value. The controller is arranged to selectively-drive a plurality of light-modulating pixels so as to display the hologram. Displaying the hologram comprises displaying each hologram pixel value on a contiguous group of light-modulating pixels of the plurality of light-modulating pixels such that there is a one-to-many pixel correlation between the hologram and the plurality of light-modulating pixels.