The present invention includes method and device for label-free holographic screening and enumeration of tumor cells in bulk flow comprising: a laser source, a micro-objective, a pinhole device and a collimating lens, a mirror, a sample chamber with a sample flow inlet on a first side of the sample chamber and a sample flow outlet connected by a microchannel, and a detector, wherein the collimated laser beam passes through microchannel and interacts with cells in the sample to generate a respective hologram at the detector, wherein a processor calculates a numerical reconstruction from the respective hologram and generates a focused image of the numerous cells using the numerical reconstruction, wherein the numerous cells are enumerated by looking at a size, a maximum intensity and a mean intensity of the focused image.

The accuracy of estimation of a focal distance in digital holography is enhanced. In an image reproduction method, a two-dimensional power spectrum is generated from an interference fringe image generated from object light and reference light, the two-dimensional power spectrum having an intensity specified by a first frequency in a first direction and a second frequency in a second direction. A one-dimensional power spectrum is generated by, for each frequency component specified by the first frequency and the second frequency in the two-dimensional power spectrum, associating the frequency component with a feature quantity, the feature quantity being calculated by aggregating a plurality of intensities corresponding to the frequency component. A focal distance between an object and a detector is estimated using a trained distance estimation model, the trained distance estimation model receiving, as input, a plurality of feature quantities included in the one-dimensional power spectrum.

Systems and methods are provided for a digital holography range Doppler receiver. The subject system transmits outgoing electromagnetic radiation to a target, and provides a first reference local oscillator (LO) beam to a first detector and a second reference LO beam to a second detector, based on the outgoing electromagnetic radiation. The system receives reflected electromagnetic radiation from the target through a first optical receiver and a second optical receiver having a smaller diameter, and determines range and velocity of the target simultaneously using an interference with the second reference LO beam. The system applies time and frequency offsets to the first reference LO beam based on the measured range and velocity to align the first reference LO beam with the reflected electromagnetic radiation, and produces an image of the target using the first reference LO beam having the applied time and frequency offsets.

A device for detecting at least one object present in a sample, the device including a light source to emit at least one incident wave at a wavelength λ, a detection volume intended to receive the object, and to receive at least one incident wave, an image sensor positioned to receive at least one scattered light wave obtained by diffraction of the incident wave on the object and a reference wave from the source and not diffracted on the object and to generate a holographic image, and a computer data processing device to digitally reconstruct the object based at least on the holographic image and the wavelength λ. The device also comprises a support comprising patterns organized to form at least one diffraction grating, the grating being periodic and having a pitch P, such that λ/2≤P≤2λ.

A lensless holographic imaging system having a holographic optical element includes: a coherent light source for outputting a first light beam and a second light beam, wherein the first light beam irradiates a first inspection plane to form first object-diffracted light; a light modulator for modulating the second light beam into reading light having a specific wavefront; a multiplexed holographic optical element, wherein the first object-diffracted light passes through the multiplexed holographic optical element, and the reading light is input into the multiplexed holographic optical element to generate a diffracted light beam as system reference light; and an image capture device for reading at least one interference signal generated by interference between the first object-diffracted light and the system reference light. The lensless holographic imaging system has a relatively small volume and relatively high diffraction efficiency.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A determination method of non-destructively and easily determining a state of an aggregate of a plurality of cells formed by three-dimensional culture is provided. A determination method according to the disclosed technology includes generating a phase difference image of an aggregate of a plurality of cells from a hologram obtained by imaging the aggregate, deriving a first index value that indicates a randomness of an array of a phase difference amount in a plurality of pixels constituting the phase difference image, and determining a state of the cells constituting the aggregate on the basis of the first index value.

A determination method of non-destructively and easily determining a state of an aggregate of a plurality of cells formed by three-dimensional culture is provided. A determination method according to the disclosed technology includes generating a phase difference image of an aggregate of a plurality of cells from a hologram obtained by imaging the aggregate, deriving a phase difference amount density by dividing a total phase difference amount that is a value obtained by integrating a phase difference amount of each of a plurality of pixels constituting the phase difference image by a volume of the aggregate, and determining a state of the aggregate on the basis of a time transition of the phase difference amount density.

The inventive concept relates to a device for detecting particles in air, said device comprising a receiver for receiving a flow of air comprising particles, a sample carrier, and a particle capturing arrangement. The particle capturing arrangement is configured to separate the particles from the flow of air for and to collect a set of particles on a surface of the sample carrier. The device further comprises a light source configured to illuminate the particles on the sample carrier, such that an interference pattern is formed by interference between light being scattered by the particles and non-scattered light from the light source. The device further comprises an image sensor configured to detect the interference pattern. The device further comprises a cleaner configured for cleaning the surface of the sample carrier for enabling re-use of the surface for collection of a subsequent set of particles.

A device for detecting particles in air; said device comprising: a receiver for receiving a flow of air comprising particles; a particle capturing arrangement configured to transfer the particles from the flow of air to a liquid for collection of a set of particles in the liquid; a flow channel configured to pass a flow of the liquid comprising the set of particles through the flow channel; a light source configured to illuminate the set of particles in the flow channel, such that an interference pattern is formed by interference between light being scattered by the set of particles and non-scattered light from the light source; and an image sensor comprising a plurality of photo-sensitive elements configured to detect incident light, the image sensor being configured to detect the interference pattern.