A method of obtaining a focus term by using a periodicity of the focus term is provided. The focus term may be used in a plurality of operation processes for processing image data.

In the present invention, by receiving three-dimensional spatial data for a three-dimensional object, calculating a size of an elemental fringe pattern for each depth and a maximum size of the elemental fringe pattern for the three-dimensional spatial data, dividing the entire resolution of an entire predetermined hologram into a plurality of segments based on the number of divisions of the horizontal direction and the vertical direction, expanding resolution for each segment based on a maximum size for the elemental fringe pattern size, calculating a plurality of segment unit holograms for each of the segments, and accumulating overlapping regions between the plurality of segment unit holograms to produce the entire hologram for the entire resolution, it is possible to provide a hologram generating apparatus and method which may generate a hologram having a higher resolution at a faster speed than the conventional one.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

Provided are an improved holographic reconstruction apparatus and method.

A holographic reconstruction method includes: obtaining an object hologram of a measurement target object; extracting reference light information from the obtained object hologram; calculating a wavenumber vector constant of the extracted reference light information, and generating digital reference light by calculating a compensation term of the reference light information by using the calculated wavenumber vector constant; extracting curvature aberration information from the object hologram, and then generating digital curvature in which a curvature aberration is compensated for; calculating a compensated object hologram by multiplying the compensation term of the reference light information by the obtained object hologram; extracting phase information of the compensated object hologram; and reconstructing 3-dimensional (3D) shape information and quantitative thickness information of the measurement target object by calculating the quantitative thickness information of the measurement target object by using the extracted phase information of the compensated object hologram.

A non-destructive iterative interferometric tomographic technique for imaging and reconstruction of phase objects as well as objects with complex permittivity and, particularly, to iterative optical diffraction tomographic (iODT) imaging and reconstruction of phase objects with high refractive index (RI) contrast, complex structures, and/or large optical path differences (OPDs) against the background, which cause multiple scattering, and applications thereof.

Examples are disclosed that relate to holographic near-eye display systems. One example provides a near-eye display device, comprising a diverging light source, an image producing dynamic digital hologram panel configured to receive light from the diverging light source and form an image. The near-eye display device also includes and a combiner comprising a holographic optical element positioned to receive light from the dynamic digital hologram panel and to redirect the light toward an eyebox, the holographic optical element being positioned between the eyebox and a view of an external environment to combine a view of the image formed by the dynamic digital hologram panel and the view of the external environment.

Method for observing a sample comprising the steps of (a) illuminating the sample using a light source, the light source emitting an incident light wave that propagates toward the sample along a propagation axis (Z); (b) acquiring, using an image sensor, an image of the sample, which image is formed in a detection plane; (c) forming a stack of images, called reconstructed images, from the image acquired in step (b), each reconstructed image being obtained by applying, for one reconstruction distance, a numerical propagation operator; and (d) from each image of the stack of images, computing a clearness indicator for various radial positions, each clearness indicator being associated with one radial position and with one reconstruction distance.

A method of calibration of a device for analyzing at least one element present in a sample, said device including: a detection assembly configured to acquire an image formed by the interference between a light source and said sample; and digital processing means configured to detect a digital position of at least one element in said sample based on said acquired image; said calibration method including the implementation of a plurality of predetermined displacements of said sample with respect to said detection assembly and, for all of said displacements, the detection of a digital position of a same element to determine the digital position and the real position matching model according to the predetermined displacements and to the digital positions of said element after each displacement.

Methods and systems for generating a high-color-fidelity and high-resolution color image of a sample are disclosed; which fuses or merges a holographic image acquired at a single wavelength with a color-calibrated image taken by a low-magnification lens-based microscope using a wavelet transform based colorization method. A holographic microscope is used to obtain holographic images which are used to computationally reconstruct a high-resolution mono-color holographic image of the sample. A lens-based microscope is used to obtain low resolution color images. A discrete wavelet transform (DWT) is used to generate a final image that merges the low-resolution components from the lens-based color image and the high-resolution components from the high-resolution mono-color holographic image.

A differentiation system for differentiating cells includes an input device configured to receive holographic image data of a microscopic particle in suspension, holographic image data processing logic for converting the holographic image data to the frequency domain by performing a Fourier transform of the holographic image data, and a recognizer configured to determine characterization features of the holographic image data of the microscopic particle in the frequency domain for characterization of the microscopic particle, the characterization features comprising rotationally invariant features.