Provided herein are bed-to-bed patient transfer sheets that are flexible, easy to assemble, attachable and re-attachable, highly portable, endlessly customizable, and able to meet the needs of the biomedical community due to the modular design features of the bed-to-bed patient transfer sheet. The described patient transfer sheets provide sufficient functionality to meet the modern needs for transporting patients to and from different transportation vehicles, hospital locations such as operating rooms, and examination rooms, or laboratory, treatment, and recovery locations in hospitals or other off-site biomedical or health-care based locations. The transfer sheets are also easily decontaminated of human waste between uses, making them reusable.