A method of extracting viable bacterial cells from a soil sample by mixing a dispersant-surfactant solution with a soil sample to form a soil slurry, adding the soil slurry to a centrifuge tube containing a density gradient medium, centrifuging the centrifuge tube to form a solvent layer above the density gradient medium, wherein the solvent layer comprises viable bacterial cells, extracting the solvent layer containing the viable bacterial cells, combining the extracted solvent layer with a PBS solution to form a PBS-cell mixture, filtering the PBS-cell mixture to form a cell filtrate, depositing the cell filtrate into a second centrifuge tube containing a quantity of the density gradient medium, centrifuging the second centrifuge tube to form a second solvent layer comprising the viable bacterial cells, and extracting the second solvent layer from the second centrifuge medium to form a second cell filtrate comprising the viable bacterial cells.

The present application provides: an emulsion production member for dividing a sample into fine droplets and dispersing the fine droplets in a dispersion medium to produce an emulsion; and a sample collection tube and a blood collection tube, each of which is equipped with the emulsion production member. The emulsion production member is provided with: a plug body, a sample injection hole, a sample storage unit, a dispersion medium injection hole, and a dispersion medium storage unit. The emulsion production member is further provided with: a discharge port; a sample flow passage; and a dispersion medium flow passage for connecting the dispersion medium storage unit and the sample flow passage to each other to cause the dispersion medium to flow in order to divide the sample circulating in the sample flow path into fine droplets by means of the dispersion medium.

A trap for horizontal flow marine sediment, useful for collecting and sampling marine sediments where horizontal currents are intense and turbulent; the trap is comprising a first inlet opening and a second outlet opening, configured to allow the inlet and the outlet of a marine current, the path between the two openings comprising: an inlet area with a divergent section, connected to the first inlet opening; a sedimentation area with a greater section with respect to the inlet area, in order to allow gravity separation of particles present in the marine current. The trap also has an outlet area whose section converges in terms of width between the section where the outlet area is connected to the sedimentation area and to the second outlet opening.

A laser module includes a plurality of light emission regions and the plurality of light emission regions emit a plurality of laser beams. An optical waveguide and a lens condense the plurality of laser beams to an identical focal point. An adjustment mechanism is configured to adjust relative positional relation between the sample stage and a condenser lens (the optical waveguide and the lens). A controller is configured to switch between a single-point irradiation mode and a multi-point irradiation mode. The single-point irradiation mode refers to a mode in which the adjustment mechanism is controlled such that the focal point of the plurality of laser beams falls on the thin film. The multi-point irradiation mode refers to a mode in which the adjustment mechanism is controlled such that the focal point does not fall on the thin film.

The application discloses a device for drawing a liquid from a process tank to be protected from contamination, including: a first receptacle for receiving the liquid withdrawn from the process tank; a first liquid line connecting the process tank with the first receptacle; and at least one first valve assembly disposed in the first liquid line and designed to block or release liquid transport through the first liquid line. The device includes at least one pressure sensor for detecting a pressure within the first receptacle. The device is designed such that a pressure difference between the first and the second ends of the first liquid line is greater than a predetermined minimum value when the first liquid line is connected with the process tank and the process tank is to be protected from contamination.

Low or no disturbance sampling can be accomplished such as through a single-platform aquatic species and habitat sampling system with data integration and rapid processing capabilities that can address the need for sampling at variable depths over varied habitats, along with the simultaneous collection of linked physical and biological data. The platform may be based on a 24-36 foot boat, and may include a net mouth opener brace for an adjustable concentrator net and smaller drift net which may be attached to an adjustable sample chamber, perhaps containing variable mesh capture nets as well as cameras, water sampling equipment, and water quality sensors integrated with a fish finder, GPS, and other monitoring and data recording equipment. The depth of the net mouth opener brace and sample chamber may be adjustable using a depth control.

A microorganism concentrator includes a water storage part that stores water sample therein and a filter that partitions the water storage part into a supply side and a drain side for the water sample. The microorganism concentrator concentrates microorganisms that are too large to pass through the filter on the supply side by moving the water sample on the supply side to the drain side of the water storage part. In the microorganism concentrator, the filter is arranged in such a way that no vertically downward force is applied to a surface of the filter on the supply side.

A device comprises a contact reaction chamber, a circulating water inlet, a clear water basin, a circulating pump, a circulating water outlet, a solid phase extractant collecting tank, a magnetic holder, an electromagnet, a solid-liquid separation area, a drain valve, a wall sprinkling water inlet, and a wall sprinkling pipe, wherein, the contact reaction chamber is in a conical shape, and utilizes hydraulic power to perform stir to ensure no dead corner exists during contact stir; the obconical solid-liquid separation area increases the action area between a magnetic solid phase extractant and the electromagnet; the solid phase extractant collecting tank is in a downwards protruding dish shape to prevent the solid phase extractant from losing.

The present application discloses a grouting consolidation sample containing internal defects and the preparation method thereof. Firstly, plasticine is employed to prepare a wax model with the desired defect shape, then a fixing device is used to fix the wax model onto a specific position in a casting mould of consolidation and thereafter a slurry is poured to form a grouting consolidation sample, after the grouting consolidation sample reaches the predetermined strength, the grouting consolidation sample containing the wax model is put into an oven to heat at a temperature above the melting point of wax to demolish the structure of the wax model to form a grouting consolidation sample with internal defects.

The exemplary system and method configured to perform benthic flux incubation and measurement in an on-going continuous manner via motorized or non-motorized actuators that moves one or more sampling chambers (i) between different sampling regions or (ii) maintains the sampling chamber over a single region, for long term repeated measurements. The exemplary system and method can incubate and sample over multiple benthic sampling regions to acquire repeated measurements of both (i) fluxes and (ii) background processes and conditions (e.g., via ambient water incubations or ambient water concentration measurements), the results of which can be combined and processed by the exemplary system to generate data requiring minimal post-processing.