A phantom design method includes a correction step and a calculation step. In the correction step, an absorption spectrum of a target of spectroscopic measurement by a near infrared spectrometer is corrected based on a refractive index of the target and a refractive index of a resin used as a base material of a phantom to generate a corrected absorption spectrum. In the calculation step, based on an absorption spectrum of the resin and an absorption spectrum of each of N types of dyes, a concentration of each of the N types of dyes to be contained in the base material is calculated such that an absorption spectrum of the phantom constituted by the base material containing the N types of dyes approximates the corrected absorption spectrum in a predetermined wavelength range of a near infrared region.

A calibration standard for determining an intensity decay related to an evanescent field generated close to the interface between a sample to be tested and a substrate on which the sample is to be deposited, preparation and analysis methods and use thereof.

A simulator and test method of polarization transmission in sea fog belong to the technical field of polarization transmission characteristics. The simulator comprises an emitting device, a receiving device, a main box body, an automatic light alignment system, a water fog layer calibration system, a salt fog layer calibration system, and an aerosol layer calibration system. In accordance with the present disclosure, the automatic alignment of polarized light and a relatively stable sea fog environment are simulated in an indoor environment, the accuracy of indoor simulation is improved, the conformity between measured data and a theoretical simulation result is superior to 80%, and a reliable technical support is provided for the sea surface detection.

A calibration disc for providing uniform irradiance to an optical sensor system includes a first major surface, a second major surface opposite the first major surface, and an edge surface extending around a circumference of the calibration disc. The first major surface is fully reflective and partially diffuse, the second major surface is partially reflective and partially diffuse, and the edge surface is fully reflective and partially diffuse and has an entrance aperture positioned at the edge surface and configured to receive light into the calibration disc. The first major surface, the second major surface and the edge surface are configured to scatter the light received by the entrance aperture within the calibration disc. The second major surface is configured to emit at least some of the light with uniform irradiance.

The present invention relates in one aspect to a ballast water analysis system comprising fluorometer and light scattering meter. The fluorometer comprises a first light source arranged to illuminate a first ballast water sample for obtaining a first fluorescence measurement on a first ballast water sample. The light scattering meter comprises a second light source arranged to illuminate a second ballast water sample with a second light beam and first and second photodetectors arranged to receive light at respective angles relative to a direction of the second light beam. The second and third photodetectors are configured to receive scattered light resulting from interaction between light from the second light source and matter, such as viable or non-viable microorganisms and other particles, in the second ballast water sample.

The present invention relates to a method for determining a characteristic number for characterizing the quality of a shade setting of a paint in relation to a color reference, characterized in that colorimetric coordinates of the paint and of the color reference are determined with a spectrophotometer for a number of measurement geometries and wherein respective color differences are calculated and standardized from the colorimetric coordinates of the paint and of the color reference for each measurement geometry of the number of measurement geometries, and where a group of characteristic values calculated from the respective standardized color differences is assigned a scale value for determining the characteristic number by means of an assignment rule to be provided in advance.

Examples of an optical standard and a calibration apparatus for calibrating or characterizing a spectroscopy system using such optical standard are disclosed. The optical standard can comprises a mixture of acetaminophen and barium sulfate, wherein a mass of the acetaminophen in the mixture is being less than a mass of the BaSO4. Such optical standard can be used in a calibration device for calibrating or characterizing a spectroscopy system. The calibration device can comprise a substrate base with a top surface and a bottom surface. The top surface can include a section for receiving the optical standard sample. The receiving section can be adhesive. The calibration device can further comprise a film that can be attached to the top surface of the substrate base to cover at least the section of the substrate where the optical standard is being placed. The calibration device can be disposed after the calibration measurements are completed. The optical standard and the calibration apparatus using the optical standard can be used as a wavelength calibration standard to calibrate a Raman system, a reflectance reference standard for a reflectance spectral measurement or for a reliability check in a fluorescence spectral system.

An optical system of an optical analysis device is easily evaluated with high accuracy. There is provided a method of evaluating an optical analysis device including an optical system A capable of forming a confocal volume C at a focal position by condensing excitation light B, the method including the steps of: placing, at the focal position of the optical system A, a phantom sample in which two or more types of solid members having different fluorescent substance concentrations are arranged adjacent to each other; irradiating the phantom sample 1 with excitation light through the optical system A while relatively moving the confocal volume C formed by the optical system A and the phantom sample in an arrangement direction of the solid members; detecting fluorescent light generated in the solid members placed in the confocal volume C; and evaluating the optical system A based on the detected fluorescent light.

An oxygen sensing system comprises a substrate structured to communicate optical signals. An oxygen sensing layer is disposed on the substrate and comprises an oxygen sensing molecule in a matrix in a first unexcited state and formulated to: (a) be excited by a first optical signal to move to a second state; (b) be quenched in the second state by oxygen; and (c) emit a second optical signal corresponding to an amount of oxygen. A protective layer, disposed on the oxygen sensing layer, includes at least one of i) an oleophobic layer and ii) an anti-fouling layer. A controller is optically coupled to the substrate and structured to generate the first optical signal, receive the second optical signal and determine oxygen concentration from the second optical signal.

The present invention relates to a fluorescent polymer cast on the surface of wells of a plate and a plate comprising the fluorescent polymer. The plate is used in a method for calibrating read-out values of plate readers. The method for calibrating the plate readers can effectively reduce the deviation of read-out values among different plate readers.