Disclosed is a method for diagnosing a target material by using a first substrate printed with a first nanoparticle. A second nanoparticle, which is bonded to a compound to be bound to the target material, is positioned at a distance adjacent to the first substrate.

A method is for detecting a biomarker within a sample of blood. The method may include processing the sample of blood with a microfluidic blood plasma separator and a plasmonic array biosensor, and flowing the sample of blood over a sensing surface of the plasmonic array biosensor. The sensing surface of the plasmonic array biosensor may have an ssDNA aptamer against the biomarker. The method may further include binding the biomarker in the sample of blood to the ssDNA aptamer of the plasmonic array biosensor, and detecting the biomarker in the sample of blood based upon LSPR altering a reflected optical signal from the plasmonic array biosensor.

The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands and protein resistant groups, preferably polyethylene glycol (PEG), directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.

The present disclosure relates generally to the field of neurology. In particular, the disclosure relates to a method of detecting a neurodegenerative disease in a subject and methods of treatment thereof. The methods include detecting the level of an exosome-bound aggregated biomarker in a sample obtained from the subject, wherein an increased level of the exosome-bound aggregated biomarker as compared to a reference indicates that the subject is suffering from a neurodegenerative disease. Also described are methods for detecting a subject at risk of developing amyloidosis or a neurodegenerative disease, methods for detecting and treating amyloidosis or a neurodegenerative disease in a subject, and methods of determining the aggregation state of a biomarker in a sample.

A blood biomarker analysis systems providing fast biomarker identification includes a multimodal bioassay device having a biosensor within a portable pipette-shaped device and using nanoplasmonic barcode detectors, such as formed of antibody conjugated gold nanoparticle arrays (AuNPs), capable of capturing any of a plurality of biomarkers. The biomarker analysis system further includes the pipette-shaped device being smartphone-connected and portable to form a highly accurate, point-of-care bioassay device.

Disclosed herein are nanostructured plasmonic materials. The nanostructured plasmonic materials can include a first nanostructured layer comprising: a first layer of a first plasmonic material permeated by a first plurality of spaced-apart holes, wherein the first plurality of spaced apart holes comprise a first array; and a second nanostructured layer comprising a second layer of a second plasmonic material permeated by a second plurality of spaced-apart holes, wherein the second plurality of spaced apart holes comprise a second array; wherein the second nanostructured layer is located proximate the first nanostructured layer; and wherein the first principle axis of the first array is rotated at a rotation angle compared to the first principle axis of the second array.

Biosensing platform for simultaneous, multiplexed, high throughput and ultra-sensitive optical detection of biomarkers labelled with plasmonic nanoparticles, the platform being provided with a biosensor, a broadband and continuous spectrum illumination source, an optical detector for simultaneously capturing spatially resolved and spectrally resolved the scattering signal of each individual nanoparticle, an autofocus system and an optical system adapted to collect the scattered signal of the biosensor's surface onto the optical detector, the platform being provided with translation means for the optical system and/or the biosensor, such that the optical system and the biosensor can be displaced relative to each other in the three dimensions, and wherein the processing means are adapted to: i) simultaneously capture spatially and spectrally resolved scattering signals from each nanoparticle individually, and ii) to analyze these signals simultaneously with the capture process.

A method for optically detecting biomarkers in a biosensor is disclosed, wherein the optical detection obtains spatially and spectrally resolved optical signals from a sample on a biosensor, and one or more of these spatially and spectrally resolved optical signals can be analyzed in parallel with image acquisition. The image analysis comprises reading data of the acquired images, correcting them to reduce inhomogeneities and noise, localizing particles in the images, characterizing each particle individually to obtain its position and characterization parameters, and classifying the particles based on their characterization parameters. Using the number of particles per class for all the acquired images of the sample, a statistical value is calculated per sample and each statistical value is correlated with an indication of the presence of a biomarker in the sample.

A measurement system for obtaining information about a sample comprises an excitation-beam source configured for irradiating the sample with an excitation-beam. The measurement system comprises a probe unit configured for exposing the sample to a probing radiation or a probing field, and a detection unit configured for obtaining a first information about an interaction of the probing radiation or the probing field with the sample, if a plasmon or plasmon-polariton was excited by the excitation-beam, and obtaining a second information about an interaction of the probing radiation of the probing field with the sample, if a plasmon or plasmon-polariton was not excited by the excitation-beam.

Detection system for detecting at least one extracellular vesicle in a microfluid, including a broadband light source, collimating and focusing optics, a spectrophotometer, a microfluid apparatus and an active sensing element positioned inside the microfluid apparatus, the active sensing element including a substrate, a thin metal layer deposited on the substrate and a dielectric waveguide layer deposited on the metal layer, the light source generating at least one incident beam of light in the near infrared region, the metal layer and the waveguide layer each include a plurality of waveguides, the collimating optics collimates the incident beam of light on the substrate via the coupler, the focusing optics receives at least one reflection of the incident beam of light and provides the reflection to the spectrophotometer, the active sensing element causes surface plasmon waves in the microfluid when the microfluid is injected into the microfluid apparatus and the spectrophotometer detects resonance wavelength shifts in the reflection according to the surface plasmon waves thereby detecting the presence of the extracellular vesicle in the microfluid.