There are provided a localized surface plasmon resonance sensing chip in which a linewidth of an absorption spectrum originating from localized plasmon resonance is narrow and with which a peak wavelength shift of an optical spectrum accompanying a change in refractive index of a surface can be accurately measured, and a localized surface plasmon resonance sensing system using this sensing chip. A sensing chip 10 includes a base 14 having a flat-plate shape, a plurality of protruding portions 16, and a metal layer 18 covering each front surface of the plurality of protruding portions 16. The protruding portions 16 each have a shape like a semi-oblate spheroid which is one of three-dimensional parts obtained by dividing an oblate spheroid in half along an equatorial plane and are arranged such that a divided surface 16a of the semi-oblate spheroid faces a front surface 14a of the base 14. A sensing system includes the sensing chip 10, a light source irradiating a detection region of the sensing chip 10 with light, and a photodetector detecting the optical spectrum of light emitted from the light source and then reflected in or transmitted through the detection region of the sensing chip 10.

Apparatuses and methods for spectroscopy using multiple resonance modes are provided. Multiple resonance modes may be used for bulk sensing and/or surface sensing applications. A plasmon waveguide resonance sensor is provided for multimode spectroscopy. The sensor includes a dielectric layer; and a metallic layer coupled to the dielectric layer. The sensor is configured to provide: a first resonance mode for bulk sensing, in response to light of a given wavelength; and a second resonance mode for surface sensing, in response to light of the given wavelength. The first and second resonance modes have different polarizations. Surface plasmon resonance assemblies are provided having a grating coupled to a surface plasmon resonance sensor, the grating being a dielectric grating or a metallic grating. The grating, in response to light, provides various resonance modes having at least two different polarizations for bulk and surface sensing.

A liquid-sample component analysis method includes a dripping step of dripping a liquid sample onto a flat horizontal surface, a drying step of drying a liquid droplet of the liquid sample formed on the horizontal surface while keeping the liquid droplet still so as to obtain a plurality of concentrically-arranged ring-shaped deposits formed on the horizontal surface and composed of components having different particle diameters, and a measuring step of measuring a vibrational spectrum in each region including only one of the deposits so as to individually acquire vibrational spectra of the plurality of deposits.

The invention relates to a metamaterial nano-sensing system, and in particular to a high-sensitivity metamaterial nano-sensing system with an ultra-narrow line width spectral response. The system includes an input light path, a metamaterial nano-sensing unit and an output light path which are sequentially provided along a direction of a light path, and the metamaterial nano-sensing unit includes a Bragg grating and a metallic periodic array arranged above the Bragg grating. The nano-sensing system provided by the invention has an ultra-narrow line width spectral response, so that sensitivity of a nanosensor is effectively improved, and broad application prospect in the fields of portable biosensing, drug development and detection, environment monitoring and the like is ensured.

A nano-sensing device is disclosed for high throughput nucleic acid sequencing. The device is a silicon chip, having a silicon substrate with a groove or well, a microfluidic channel, and a polarized multilayer graphene sheet with nanopores about 0.3 to 3.0 nm wide. A base substrate layer of silicon nitride, alumina, or boron nitride may be employed. Ionic forces cause a nucleic acid strand to translocate through the nanopore. The specific nucleobases comprising the nucleic acid can be detected and assigned using localized surface plasmonic resonance (LSPR) and laser or diode light source and optical detector. Alternatively, nucleobases translocating through the nanopores can be detected and assigned by ionic current detection with a patch-clamp amplifier. Also disclosed are arrays of inventive devices.

Structures and methods for Surface-Enhanced Raman Spectroscopy (SERS) are presented. In some embodiments, a SERS structure includes a ground plate with a spacer layer disposed thereon. A first plurality of metallic nanostructures is disposed on the spacer layer such that a portion of the spacer layer is exposed in gaps formed between the nanostructures of the first plurality of metallic nanostructures. In some embodiments, a first metallic layer is annealed to form the first plurality of metallic nanostructures. A second plurality of metallic nanostructures is disposed on the spacer layer in the gaps of the first plurality of metallic nanostructures. In some embodiments, a second metallic layer is annealed to form the second plurality of metallic nanostructures.

In one example, an analysis chip includes a substrate for surface-enhanced spectroscopy including an ordered nanostructure surface to receive a liquid including a number of analytes. The received liquid is to be guided by the ordered nanostructure surface over the substrate to separate the number of analytes.

A detection method including forming a complex by binding, to a target substance, a first substance immobilized to a metal particle with magnetism and a second substance labeled with a fluorescent material, moving the complex by applying a magnetic field, illuminating the complex during movement with excitation light of a predetermined wavelength, the excitation light causing the fluorescent material to emit fluorescence, the fluorescence being enhanced by localized surface plasmon resonance that is produced by the metal particle, capturing the enhanced fluorescence over time and obtaining two-dimensional images, and detecting the target substance in accordance with a light spot included in each of the two-dimensional images, the metal particle including an inner core made of a magnetic material and an outer shell covering the inner core, the outer shell being made of a nonmagnetic metal material that produces the localized surface plasmon resonance.

Improved multilayered magneto-optic-plasmonic (“MOP”) films that are used in connection with surface plasmon detection that have a first layer comprising titanium, a second layer selected from a group consisting of gold and silver, a third layer comprising cobalt, and a fourth layer comprising gold are disclosed. In an embodiment, the film has a first titanium layer with a thickness of approximately 2 nm, a second gold layer with a thickness of 35 nm, a layer of cobalt having a thickness of approximately 8 nm and a fourth gold base layer having a thickness of approximately 10 nm.

A surface enhanced luminescence analyte interrogation stage may include a substrate and an array of pillars projecting from the substrate. Each of the pillars may include a polymeric post formed from a first material and a cap on the polymeric post. The cap has a plasmonic surface and is formed from a second material different than the first. A sacrificial coating covers the cap of each of the pillars.