An apparatus for performing photochemical measurements on a liquid or liquid-containing sample includes a light generating system, an optical system configured for directing light from the light generating system towards the sample and a sensing system for measuring at least one optical property of the sample, wherein the light generating system is configured for generating light with intensity levels adjustable within a range, spanning at least three and preferably at least five orders of magnitude; and in that the optical system is configured for directing light from the light generating system towards the sample so as to generate a substantially uniform light intensity level, within a cylindrical region of the sample having a height larger than or equal to five time its radius.

A system (100) and a method for detecting fluorescence is disclosed. The system (100) essentially comprises a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, a chamber for holding said labelled sample during said LASER irradiation, a reflective layer (108) positioned to reflect said electromagnetic radiation, and a detector (112) positioned to detect and amplify said electromagnetic radiation. The method essentially comprises the steps of providing a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, providing a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, providing a chamber for holding said labelled sample during said LASER irradiation, providing a reflective layer (108) positioned to reflect said electromagnetic radiation, providing a detector (112) positioned to detect and amplify said electromagnetic radiation, irradiating said sample with said LASER beam and analyzing said amplified electromagnetic radiation from said detector (112) with a signal processing block (114).

Disclosed is a PCR diagnosis apparatus, which includes a transmitter including a multi-wavelength light source for outputting a first light source signal and a second light source signal having different wavelengths, and that applies the first light source signal and the second light source signal to a PCR chip including samples each including a plurality of DNAs, a code generator that generates first code signal and second code signal corresponding to the first light source signal and the second light source signal, respectively, and which are orthogonal to each other, and a receiver that performs a dot product on fluorescent data and each of the first code signal and the second code signal, wherein the fluorescent data include a first fluorescent signal and a second fluorescent signal emitted from a phosphor attached to each of the plurality of DNAs.

The present invention relates to a method of predicting a performance characteristic of a plant or yeast hydrolysate, wherein a plant or yeast hydrolysate sample is measured with 2D fluorescence spectroscopy in powder form. Said method comprises the steps for providing a model based on a predetermined value of a manufacturing parameter of interest. For this purpose a training set consisting of predetermined manufacturing parameter of interest (e.g volumetric productivity parameter, virus titer or cell number) and fluorescence spectroscopic data is used. The fluorescence spectroscopic data is correlated to the values of the manufacturing parameter of interest to obtain a calibration model/model parameters by applying multivariate data analysis. This calibration model is being used to predict the manufacturing parameter of interest for new samples dedicated for the manufacturing process. This prediction is used for a decision to accept or reject the lot which corresponds to the respective sample for use in the manufacturing process or for further evaluation depending on the pre-defined range of the manufacturing parameter of interest. The invention further relates to a method for preparation of a cell culture medium, preferably an animal protein free cell culture medium, a method for cultivating cells, a method for producing a recombinant target protein, a method for producing an immunogenic composition, whereby the above method of predicting a performance characteristic has been used for selecting the plant or yeast hydrolysate to be used in the manufacturing process.

An LED spectrofluorometer (100) for analysis of an object (101) includes a light excitation element (11, 112, 113) suitable for illuminating a study zone (101B) of the object with an excitation light beam (1), and an optical routing element (121, 122, 123, 124) suitable for collecting a fluorescent light flux (2) emitted by the study zone excited by the excitation light beam and for routing the fluorescent light flux to an optical spectrometer (131) for analysis of the light spectrum thereof. The light excitation element includes a first light-emitting diode (111) and a second-light emitting diode (112), the first light-emitting diode emitting at a first wavelength (λ1) between 250 and 300 nm and the excitation light beam being formed from one or other of the light beams generated by each light-emitting diode.

The present invention provides a multi-channel fluorescence detecting system for detecting a plurality of fluorescence labeled analytes. The multi-channel fluorescence detecting system comprises a light source, a light filter device, a dual branch light guide tube, and a detector. The light source comprises a plurality of sub light sources for respectively providing an excitation light. The plurality of sub light sources are a plurality of single color Light emitting diodes (LEDs) which can be selectively turned on or off. The light source generates a plurality of lights with full width at half maximum (FWHM) wavelengths formed in a non-overlap manner. With the disposition of the plurality of sub light sources, the accuracy for detecting the specific analytes is raised, the light flux with a specific wavelength band is effectively raised (without raising the light flux of the full wavelength band), the structure is simplified, and the manufacturing cost is decreased.

A convective polymerase chain reaction apparatus and an optical detecting method thereof are provided. The optical detecting method includes the following steps. A substance in a tube to be tested is heated. At least two monochromatic lights are provided and are combined using a light combining element to irradiate the tube to be tested. At least two excited lights generated by exciting the substance in the tube to be tested by the at least two monochromatic lights are sensed.

There are provided a control device of an image reading apparatus, an operation method and an operation program thereof, and an image detection system capable of quickly and easily outputting an image having an appropriate density for analysis from an image reading apparatus. An image receiving unit receives a pre-image output in pre-scanning performed before main scanning for outputting a main image for analysis in an image reading apparatus. A region information receiving unit receives information of a region in the pre-image designated by a user. A calculation unit calculates an appropriate voltage value that is a voltage value of the photomultiplier at which a density of the region becomes an appropriate density for analysis. A scanning conditions setting unit sets the appropriate voltage value as temporary scanning conditions of main scanning.

Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

A method measuring the phase transition characteristics of a macromolecule, the method comprising: generating a stream of micro-droplets comprising at least one constituent, of which one constituent comprises the macromolecule, varying the conditions in the micro-droplets; and measuring the relative concentrations of the constituents of, and the phases of the macromolecule present in, the micro-droplets.