Devices and methods for recording dynamics of cellular and/or biochemical processes, including a device including one or more dispersive elements configured to receive a pulsed laser beam with a spectrum of different wavelengths and disperse the spectrum of the pulsed laser beam; and one or more first elements configured to receive the dispersed spectrum of the pulsed laser beam, and generate a multiphoton excitation area in a biological sample by re-overlapping in time and space the dispersed spectrum of the pulsed laser beam on an area in the biological sample, wherein the device is configured to record at high speed changes of cellular and biochemical processes of a population of cells of the biological sample based on generation of the multiphoton excitation area in the biological sample.

The present invention relates to relates to a method of optimizing a plastic composition formed from a plurality of resin feedstocks. A plurality of resin feedstocks are provided. The plurality of resin feedstocks are blended to form the plastic composition. One or more properties of the plastic composition, including radiation absorption, radiation transmission, gas evolution, radiation fluorescence, or melting properties, are measured. The ratio of the plurality of resin feedstocks being blended into the plastic composition is adjusted, based on said measuring, to form an optimized plastic composition. A system for performing the method is also disclosed.

A method of spatially measuring a plurality of nano-scale structures in a sample comprises the steps of: marking the individual structures at different locations with fluorescent markers, coupling the individual structures to individual positioning aids whose positions in the sample are known, exciting the fluorescent markers with excitation light for emission of fluorescence light, wherein an intensity distribution of the excitation light has a local minimum, arranging the local minimum at different positions in a close-up range around the position of respective positioning aid whose dimensions are not larger than the diffraction limit at the wavelength of the excitation light, registering the fluorescence light emitted out of the sample separately for the individual fluorescent markers and for the different positions of the minimum, and determining positions of the individual fluorescent markers in the sample from the intensities of the fluorescence light registered.

An optochemical sensor comprises a measuring element excitable by the light of an excitation light source and in contact with a medium to be measured, and a measuring arrangement including at least one excitation light source and a detector as well as a hood separating the measuring arrangement from the measuring element, wherein the excitation light source and the detector are fixed to a base plate arranged in parallel with the measuring element, the hood, the excitation light source and the detector are separated from one another by at least a portion of the material thickness of the hood, and light from the excitation light source through an optical waveguide impinges on the measuring element at such an angle that fluorescence light emitted by the measuring element impinges perpendicularly on the detector.

A reaction processing apparatus includes: a reaction processing vessel; a first fluorescence detection device that irradiates a sample with first excitation light and detects first fluorescence produced from the sample; and a second fluorescence detection device that irradiates a sample with second excitation light and detects second fluorescence produced from the sample. The wavelength range of the first fluorescence and the wavelength range of the second excitation light overlap at least partially. The first excitation light and the second excitation light flash at a predetermined duty ratio d. The phase difference between the flashing of the first excitation light and the flashing of the second excitation light is set within a range of 2π(pm−Δpm) (rad) to 2π(pm+Δpm) (rad) or within a range of 2π[(1−pm)−Δpm] (rad) to 2π[(1−pm)+Δpm] (rad), where pm=d−d2 and Δpm=0.01*pm.

The present invention relates to fluorescence microscopy and specifically to improvements of method for and a corresponding fluorescence microscopy system for allowing separate detection of a plurality of fluorochromes.

An adjustable multi-wavelength lamp is described. The lamp can include a plurality of emitters. The emitters can include at least one ultraviolet emitter, at least one visible light emitter, and at least one infrared emitter. The lamp can include a control system for controlling operation of the plurality of emitters. The control system can be configured to selectively deliver power to any combination of one or more of the plurality of emitters to generate light approximating a target spectral distribution of intensity.

First and second filter magazines in each of which plural filters having different transmission wavelengths from each other are arranged in a row are provided, and the first and second filter magazines are arranged next to each other in one direction. A light detection unit in which plural photomultipliers of first and second photomultipliers, each of which detects light that has passed through at least one of the filters included in the first and second filter magazines, are arranged in the arrangement direction of the filters is provided, and the light detection unit is placed in the one direction in such a manner to be parallel to the first and second filter magazines. The apparatus is configured in such a manner that the first and second filter magazines and the light detection unit are movable in the arrangement direction of the filters.

A subject-to-be-examined support unit that supports a subject to be examined, a light source unit that outputs light entering the subject-to-be-examined support unit from a side opposite to a side by which a sample is supported, a fluorescent plate that is illuminated with the light that has been output from the light source unit and passed through the subject-to-be-examined support unit and the sample, and emits fluorescence, a photomultiplier that detects fluorescence that has been emitted from the fluorescent plate and passed through the subject-to-be-examined support unit and the sample, and a plate support unit that supports the fluorescent plate are provided. The plate support unit is structured in such a manner that a distance between the subject-to-be-examined support unit and the fluorescent plate is changeable by moving the fluorescent plate in a direction closer to the subject-to-be-examined support unit and in a direction away therefrom.

Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.