A fluorescent macromolecule comprising: a linear sequence-defined backbone; and a plurality of fluorophores attached to the backbone in a pre-determined order to form a fluorophore sequence, wherein the fluorophores in the fluorophore sequence are separated from one another by a distance permitting interaction between adjacent fluorophores such that the macromolecule emits fluorescence at a plurality of wavelengths when irradiated by light to form a fluorescence emission spectrum, and wherein the fluorescence emission spectrum has a profile that is determined by the fluorophore sequence.

The present disclosure provides a self-propelled pathogen detection device in which a place where a pathogen is highly likely to be present in a space such as an inside of a facility is allowed to be configured preferentially to be a target region of detection. The self-propelled pathogen detection device according to the present disclosure comprises a housing; a detection part for detecting a pathogen; a movement mechanism for moving the housing; a position acquirement part for acquiring position information representing a current position of the housing in a space; and a control part which determines a target region in the space on the basis of traffic line information on a person in the space, and controls the movement mechanism to move the housing in the target region on the basis of the position information. The detection part detects the pathogen in the target region.

Disclosed is an apparatus and method for fluorescence excitation and detection. The apparatus comprises one or more light sources for providing excitation light for fluorescence excitation at an observation spot along an optical axis for excitation, an optical collection element for collecting fluorescence light generated by the excitation light at two or more different observation spots into two or more different measurement channels with an optical axis for collecting non-parallel to the optical axis for excitation of each of the one or more light sources, and, for each of the two or more measurement channels and thereby for each of the two or more observation spots, a dedicated optical detector for detecting fluorescence from the fluorescence light collected by the optical collection element.

A method for identifying an origin of Chrysanthemi flos is provided, which belongs to the technical field of chemical analysis and detection, and comprises the following steps: mixing Chrysanthemi flos extract with aluminum ion solution, and gold nano-clusters (AuNCs) solution in a solvent, standing for reaction, detecting fluorescence intensity of Chrysanthemi flos, comparing the fluorescence intensity of Chrysanthemi flos to be detected with that of Chrysanthemi flos from a target origin, and determining whether they are from a same origin. According to the application, excited-state intramolecular proton transfer effect between 3-hydroxyflavone derivatives of Chrysanthemi flos and aluminum ions is utilized to enhance the fluorescence of 3-hydroxyflavone derivatives, where AuNCs combines aluminum ions to enhance aggregation-induced fluorescence, and reacts with flavonoids to quench their fluorescence; and visual characterization and traceability of Chrysanthemum morifolium quality are achieved by further comparing obvious rich fluorescence color changes before and after the reaction.

A fluorescence observation apparatus (1) includes an irradiation unit (10) that applies a plurality of kinds of excitation light (L11, L21) of mutually different wavelengths to a plurality of spatially or temporally different positions in a biological sample (5) that is labeled with a composite phosphor containing two or more kinds of fluorescent molecules (A, B) at a predetermined composition ratio, a detection unit (20) that detects fluorescence (L12, L22) generated at each of the plurality of positions by application of the irradiation unit (10), and a calculation unit (33) that determines a distribution of pieces of the composite phosphor on the basis of a fluorescence signal (S1, S2) that is obtained from a detection result of the detection unit (20) and that shows a fluorescence intensity corresponding to a position in the biological sample (5) of each piece of the fluorescence (L12, L22).

A sample inspection device includes a filter unit having a plurality of optical filters configured to transmit lights of different wavelength ranges, a light source unit configured to irradiate light to any one of the optical filters, a stage for placing a sample, and a reflector oriented to reflect light transmitted through one of the optical filters to the stage and to reflect light emitted from the sample on the stage to another one of the optical filters. The device also includes a detection unit for detecting light emitted from the sample on the stage through light transmitted through the another one of the optical filters, an actuator for moving the filter unit, and a control unit configured to control the actuator to move the filter unit when the detection unit receives the light passing through the another one of the optical filters.

This disclosure relates generally to detecting multiple biomarkers on or within a sample, though more specifically, to detecting individual detection moieties within a plurality of detection moieties.

An inspection apparatus is an inspection apparatus includes an excitation light source that generates excitation light to irradiate the object, a dichroic mirror that separates fluorescence from the sample by transmitting or reflecting the fluorescence according to a wavelength, a camera that images fluorescence reflected by the dichroic mirror, a camera that images fluorescence transmitted through the dichroic mirror, and a control apparatus that derives color irregularity information of a light-emitting element based on a first fluorescence image acquired by the camera and a second fluorescence image acquired by the camera, and an edge shift width corresponding to a width of a wavelength band in which transmittance and reflectance change according to a change in wavelength in the dichroic mirror is wider than a full width at half maximum of a normal fluorescence spectrum of the light-emitting element.

There are provided infrared imaging systems and methods for imaging a sample with fluorescent markers. The system includes a light source configured to illuminate a sample-contacting surface. The light source includes first and second illumination modules, each configured to project a corresponding first and second infrared illumination beam towards a sample holder, the infrared illumination beams interacting at an imaging plane to define an illumination area having a rectangular and homogeneous power profile. The system also includes a control unit operatively connected to a motor assembly and to an optomechanical mechanism. The control unit is configured to superimpose the sample plane and the imaging plane at any of the multiple locations within the enclosure. The system includes a detector configured to receive light emitted by the fluorescent markers of the sample upon illumination of the same in the imaging plane when the sample plane is superimposed with the imaging plane.

Provided are a system for monitoring a hydroxyl radical scavenging index in water using a real-time multi-fluorescence analyzer and a parallel factor analysis apparatus and a method therefor, wherein the system monitors the hydroxyl radical scavenging index in water using the real-time multi-fluorescence analyzer and the parallel factor analysis apparatus, whereby it is possible to monitor the characteristics of an organic material in target water through a continuous flow analysis method without using an existing indicator material, rhodamine B. In addition, in a water treatment system having an advanced oxidation process (AOP) applied thereto in which ozone, ultraviolet rays, hydrogen peroxide, and the like are combined, it is possible to simply calculate the hydroxyl radical scavenging index in the target water through an organic material characteristic index for each component obtained by classifying the characteristic structure of the organic material in water using real-time fluorescence analysis by means of a parallel factor (PARAFAC) model. Accordingly, the amount of chemical injection and the amount of ultraviolet irradiation, which are process control variables, can be controlled, and under given operating variable conditions, the removal rate of a target material in water is predicted, whereby the system can also be used as a diagnostic tool for process evaluation in the advanced oxidation process. Furthermore, the system can provide operational convenience that enables process control while reducing the amount of power consumed in the advanced oxidation process even though the type of target material and the water quality characteristics of raw water change.