The present invention relates to a real-time fluorescence imaging sensor for measuring glutathione in cell organelles and a method for fabricating the same. More specifically, the present invention relates to a novel compound for measuring glutathione in cell organelles, a method for preparing the novel compound, a real-time fluorescence imaging sensor for measuring glutathione in cell organelles, which comprises the novel compound, a method for fabricating the imaging sensor, and a method of measuring glutathione in cell organelles by use of the imaging sensor. When the composition comprising the compound according to the present invention is used, it can measure the antioxidant activity of the organelle mitochondria or Golgi apparatus in living cells, particularly stem cells, and can screen highly active stem cells based on the results obtained by measuring the antioxidant activity of the cell organelle.

A method for measuring process parameters in liquid cultures in a plurality of microreactors of at least one microtiter plate includes continuously agitating the liquid cultures using an orbital agitator at least until the reaction is completed in all the microreactors. In order to allow process parameters also of such substances which themselves do not have any fluorescence activity to be measured with relatively low complexity and within a short time, 2D fluorescence spectra are recorded in a plurality of in particular different liquid cultures in the microreactors of agitated microplates. A device for carrying out the method is also disclosed.

High-throughput hyperspectral imaging systems are provided. According to an aspect of the invention, a system includes an excitation light source; an objective that is configured to image excitation light onto the sample, such that the excitation light causes the sample to emit fluorescence light; a channel separator that is configured to separate the fluorescence light into a plurality of spatially dispersed spectral channels; and a sensor. The excitation light source includes a light source and a plurality of lenslet arrays. Each of the lenslet arrays is configured to receive light from the light source and to generate a pattern of light, and the patterns of light generated by the lenslet arrays are combined to form the excitation light. The objective is configured to simultaneously image each of the patterns of light to form a plurality of parallel lines or an array of circular spots at different depths of the sample.

The present disclosure in some aspects provides methods for the controlled merging of emulsion droplets, which can be used to assemble useful compositions such as droplets (e.g., stabilized micelles) containing a precise combination of analytes and/or analytical reagents. In some embodiments, disclosed herein is a method, e.g., for detecting the presence/absence, a level or amount, and/or an activity of an analyte in a sample, comprising merging two or more emulsion droplets such that an interaction between an analyte and an analyte-interacting reagent occurs in the merged droplet. The two or more emulsion droplets may be merged using a method for the controlled merging of emulsion droplets disclosed herein.

A measuring apparatus includes a flow cell through which a sample containing particles flows, a light source for irradiating light on the sample flowing through the flow cell, a fluorescence detector for detecting the fluorescence generated from the sample irradiated with light from the light source, and a control unit for flowing a positive control sample containing a fluorescent dye through the flow cell, measuring the fluorescence generated from the positive control sample irradiated by the light from the light source via the fluorescence detector, comparing the obtained measurement value and a reference value, and adjusting the detection sensitivity of the fluorescence detector according to the comparison result.

A method of microscopy comprises collecting an emission light; symmetrically dispersing the collected emission light into a first order (“1.sup.st”) light and a negative first order (“−1.sup.st”) light using a grating; wherein the 1.sup.st light comprises spectral information and the −1.sup.st light comprises spectral information; capturing the 1.sup.st light and the −1.sup.st light using a camera, localizing the one or more light-emitting materials using localization information determined from both the first spectral image and the second spectral image; and determining spectral information from the one or more light-emitting materials using the first spectral image and/or the second spectral image; wherein the steps of localizing and obtaining are performed simultaneously. A spectrometer for a microscope comprises a dual-wedge prism (“DWP”) for receiving and spectrally dispersing a light beam, wherein the DWP comprises a first dispersive optical device and a second dispersive optical device adhered to each other.

Apparatus and methods are described for use with feces of a subject that are disposed within a toilet bowl. One or more light sensors receive light from the toilet bowl, while the feces are disposed within the toilet bowl. A computer processor detects a set of three or more spectral components that have a characteristic relationship with each other in a light spectrum of bile, by analyzing the received light, and detects a presence of bile within the feces, in response to detecting the set of three or more spectral components. The computer processor generates an output in response thereto. Other applications are also described.

The invention generally relates to detecting an analyte in a medium. In certain aspects, the invention provides systems and methods for detecting an analyte in a medium comprising one or more light-emitting diodes, each operating at a single wavelength in a deep ultraviolet (UV) range for excitation of a target in a medium and a plurality of semiconductor photodetectors. The system is onfigured such that each semiconductor photodetector detects only a subset of emission from the excited analyte in the medium. In some examples, systems and methods of the invention comprise a light-emitting diode and a semiconductor photodetector for detection of the absence or presence of a non-specific contaminant.

An imaging system for cell-based therapies is provided. The imagining system includes one or more optical tags configured for insertion into a cell or biological tissue, an excitation light source configured to illuminate the one or more optical tags; a detector configured to measure optical emission of the one or more optical tags; an imaging subsystem configured to determine a three-dimensional location of each of the one or more optical tags in the cell or biological tissue; and a controller in electrical communication with the excitation light source, the detector, and the imaging subsystem. Each of the one or more optical tags has a contrasting feature and includes a fluorescent material. The contrasting feature may be defined by at least one of a refractive index, shape, color, and laser emission of each optical tag of the one or more optical tags.

A processor for demixing a fluorescent-light input signal of a fluorescence microscope, the fluorescent-light input signal including at least two fluorescence emission responses that overlap in time, each of the at least two fluorescence emission responses being representative of an individual impulse response of a fluorophore to a fluorescence-triggering light pulse of a clocked time series of fluorescence-triggering light pulses, the processor: receiving a trigger signal comprising a time series of time markers, the trigger signal being representative of a clocking rate, at which the clocked time series of fluorescence-triggering light pulses is generated; and separating at least one fluorescence emission response from the fluorescent-light input signal.