Various embodiments may provide a method of illuminating a sample plane. The method may include providing an illumination subsystem, the illumination subsystem including an optical source and at least one lens, having an optic axis at an incident angle greater than 0° and less than 90° to a normal of the sample plane. The method may also include rotating the illumination subsystem about a pivot point between the optical source and the sample plane along the optic axis so that an adjusted illumination distribution generated by the illumination subsystem at the sample plane has greater symmetry compared to a reference illumination distribution generated by the illumination subsystem at the sample plane without the rotation about the pivot point.

Provided herein are systems, devices and methods for performing multianalyte detection in a biological sample, such as a human blood sample. Multiwell plates useful for performing multianalyte detection are also provided. The systems, devices and methods provided herein relate to the field of direct-to-consumer diagnostics (DTC diagnostics) and are useful, e.g., for facilitating consumer access to consumer healthcare and consumer wellness information. Other uses of the systems, devices and methods provided herein relate to the fields of medical research and drug discovery.

The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples.

The present invention provides for metallic structures comprising a sulfhydryl or amino-terminated hydrophilic coating to provide a layer of hydrophilic character on the surface of the metallic structures thereby allowing the use of low volumes of aqueous solvents of fluorophores that have the ability to “spread out” across the surfaces of the metallic structures and to provide for a more uniform surface coating of fluorophores attached to or near the metallic structures.

A method for analyzing a biochip image is provided, including: (S1) acquiring and preprocessing the biochip image to obtain a preprocessed image; (S2) performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image; and (S3) performing an enhancement processing on the deflection-corrected image, and identifying a positive or negative of an area of interest in the preprocessed image according to an image on which the enhancement processing has been performed. An apparatus (100) for analyzing a biochip image, a method for analyzing an image, a computer device (200) and a storage medium are disclosed.

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

The present disclosure relates to methods and apparatus for measuring of multiple physiological properties of biological samples, such as measuring biological flux.

A method for sample analysis that employs a signal-amplifying nanosensor is provided. An implementation of the present method may include a) obtaining a sample, b) applying the sample to a signal-amplifying nanosensor containing a capture agent that binds to an analyte of interest, under conditions suitable for binding of the analyte in a sample to the capture agent, c) washing the signal-amplifying nanosensor, and d) reading the signal-amplifying nanosensor, thereby obtaining a measurement of the amount of the analyte in the sample. In some embodiments, the analyte may be a biomarker, an environmental marker, or a foodstuff marker. Also provided herein are kits that find use in performing the present method.

The present random access PCR reactor for biological analysis, comprises of a number of PCR reactors held on a platform, and one optical system to be shared by all of the PCR reactors on the platform. The optical system is held on a traverse mechanism to move it over any one of the PCR reactors that are ready to be imaged. Other PCR reactors on the platform can be accesses and replaced. The optical system has a lightpipe and a lightguide that distributes a uniform light over all the samples held on the reactor. The lightguide of the present optical system has a set of light reflecting structures that are strategically located to uniformly reflect an incoming light towards all the samples held in the PCR reactor that is being tested.

In a sample observation device, an image acquisition unit 6 acquires a plurality of pieces of image data of a sample in a Y-axis direction, and an image generation unit generates luminance image data on luminance of the sample on the basis of the plurality of pieces of image data, binarizes luminance values of each of the plurality of pieces of image data to generate a plurality of pieces of binarized image data, and generates area image data on an existing area of the sample on the basis of the plurality of pieces of binarized image data.