A nucleic acid sequencing system may include a substrate coupled to a rotating disk. The substrate may include a plurality of nucleic acid samples. A detection system, including for example an objective and a camera, may detect sequencing events on the substrate while the substrate is rotated relative to the detection system around a rotational axis of the substrate, perpendicular to a surface of the substrate, by the actuation system.

A fluorescence imaging device includes a microplate that holds a plurality of samples generating fluorescence, a lens assembly, and an imaging unit that collectively images the plurality of samples through the lens assembly. The lens assembly includes a Fresnel lens made of a resin, a second protective plate that protects the surface of the Fresnel lens facing the microplate, a spacer that forms a gap between the Fresnel lens and the protective plate, and a frame by which the Fresnel lens, the second protective plate, and the spacer are sandwiched.

Embodiments of the current disclosure are directed to systems, methods and apparatus for evaluating single cell secretion profiles. In some embodiments, the apparatus may be configured to analyze substances expressed by a biological cell and may include a first compressible substrate, and a second substrate configured for removable sealing attachment with the first substrate. In some embodiments, upon attachment of the second substrate with the first substrate, an assembly is formed such that the open side of the plurality of chambers are covered by the second substrate, and a portion of each of the plurality of capture areas are exposed in each of the chambers.

Device and methods for detection and classification of pathogens have an imaging module, an image processing module, and a display module. The imaging module has a plurality of light sources to expose a sample to excitation radiation at various wavelengths. A detector in the imaging module synchronously captures time-resolved fluorescence emission spectra, time-resolved reflectance, and transmittance spectra at multiple spectral bands from the sample. The image processing module resolves the spectra and compares obtained spectral parameters to set of standard parameters provided in a library database to determine a match to detect and classify pathogens.

A fluorescent image analyzer stores a reference fluorescent-sample image and a subject fluorescent-sample image. The reference fluorescent-sample image is an image obtained by illuminating a reference fluorescent sample about which relation of in-plane fluorescence intensities is known with linearly polarized light and capturing a first specific polarization component of fluorescence from the reference fluorescent sample. The subject fluorescent-sample image is an image obtained by illuminating a subject fluorescent sample with linearly polarized light and capturing a second specific polarization component of fluorescence from the subject fluorescent sample. The fluorescent image analyzer is configured to determine correction coefficients to correct non-uniformity in measurement of light intensities among pixels of a captured image based on the reference fluorescent-sample image, and correct light intensities of the pixels of the subject fluorescent-sample image based on the correction coefficients.

A method of measuring rinse properties of a composition from a surface. The method includes providing a treatment composition comprising a UV fluorescent dye. The method also includes taking a first image of a surface. The method also includes applying the treatment composition to the surface after taking the first image. The method also includes taking a second image of the surface after applying the treatment composition to the surface. The method also includes rinsing the surface with water after taking the second image. The method also includes taking a third image of the surface after rinsing the surface. The method also includes analysing the first image, the second image, and the third image to quantify an amount of the treatment composition remaining on the surface after rinsing the surface.

An image sensor includes a plurality of pixels. Each pixel includes a photoelectric conversion portion, a reset gate for controlling removal of a charge accumulated in the photoelectric conversion portion, a charge accumulation portion, an accumulation gate for controlling a transfer of the charge from the photoelectric conversion portion to the charge accumulation portion, and a readout gate for controlling readout of the charge from the charge accumulation portion. The reset gate removes the charge generated in the photoelectric conversion portion by excitation light. The accumulation gate transfers the charge generated in the photoelectric conversion portion by fluorescence to the charge accumulation portion. The readout gate performs control for reading out the charge after the charge transfer is performed n times. The number n of the charge transfers is set for each pixel.

Systems and methods are provided for imaging and characterizing objects including the eye using non-uniform or speckle illumination patterns. According to the present technology, a method for characterizing at least a portion of an object may include generating, using at least one light source, one or multiple non-uniform illumination patterns on an object. The method may also include detecting, using a detector, backscattered light from the object in response to the generating. The method may further include extracting, using the detector, data representative of the backscattered light. The method may also include processing, using a processing unit, the data representative of the backscattered light to create one or more images of at least a portion of the object.

Disclosed is a fluorescence image analyzer for measuring and analyzing a sample that includes a plurality of cells in which target portions are labeled with fluorescent dyes, and the fluorescence image analyzer includes a light source configured to apply light to the sample; an imaging unit configured to take a fluorescence image of each of the cells by which fluorescence is generated by applying the light; a processing unit configured to process the fluorescence image having been taken; and a display unit. The processing unit obtains a bright point pattern of fluorescence in the fluorescence image, causes the display unit to display a plurality of positive patterns that are previously associated with at least one of a measurement item or a labeling reagent, and causes the display unit to display information of at least one of the number of abnormal cells included in the sample, a proportion of the number of the abnormal cells, the number of normal cells included in the sample, and a proportion of the normal cells, based on the bright point pattern having been obtained and the plurality of positive patterns.

A focus scan type imaging device for imaging a target object in a sample that induces aberration proposed. The device includes: a light source unit for emitting a beam; an optical interferometer for splitting the beam emitted from the light source into a sample wave and a reference wave, and providing an interference wave formed by interference between a reflection wave that is the sample wave reflected from the sample and the reference wave; a camera module for imaging the interference wave; a scanning mirror disposed on an optical path of the sample wave of the optical interferometer and configured to reflect the sample wave to cause the sample wave to scan the sample; a wavefront shaping modulator disposed on the optical path of the sample wave of the optical interferometer; and an imaging controller configured to operate in a phase map calculation mode and in an imaging mode.