A system (100) and a method for detecting fluorescence is disclosed. The system (100) essentially comprises a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, a chamber for holding said labelled sample during said LASER irradiation, a reflective layer (108) positioned to reflect said electromagnetic radiation, and a detector (112) positioned to detect and amplify said electromagnetic radiation. The method essentially comprises the steps of providing a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, providing a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, providing a chamber for holding said labelled sample during said LASER irradiation, providing a reflective layer (108) positioned to reflect said electromagnetic radiation, providing a detector (112) positioned to detect and amplify said electromagnetic radiation, irradiating said sample with said LASER beam and analyzing said amplified electromagnetic radiation from said detector (112) with a signal processing block (114).

The disclosure herein provides a device, a method and a computer-readable storage medium for quantitative phase imaging, and relates to the field of quantitative phase imaging. The specific implementation scheme is: Obtain a multiplexed interferogram of the sample, where the multiplexed interferogram is a sample beam composed of at least two beams with different wavelengths to illuminate the sample and penetrate into the cube beam splitter Combine at least two beams with different wavelengths as the reference beam, and the combined beam is the imaging image sampled by the camera; and perform phase retrieval on the multiplexed interference image to obtain each beam of the sample in the composite sample beam The phase map at the wavelength of Using the embodiments of the disclosure herein, one imaging acquisition and one phase retrieval are to acquire the phase maps of at least two wavelength channels.

In a right-handed XYZ coordinate system, a dichroic-mirror array of the present disclosure includes a first group in which m (m≥2) dichroic mirrors DA1 to DAm are arranged parallel to each other along a positive direction of an X axis and a second group in which n (n≥2) dichroic mirrors DB1 to DBn are arranged parallel to each other along a negative direction of the X axis. Incident surfaces of the DA1 to DAm and incident surfaces of the DB1 to DBn are perpendicular to an XZ plane. A slope of straight lines with normal lines of the incident surfaces of the DA1 to DAm projected onto the XZ plane are negative, and a slope of straight lines with normal lines of incident surfaces of DB1 to DBn projected onto the XZ plane are positive.

The present invention relates to a detector for measuring fluorescence in a liquid sample and to devices for biochemical analyses comprising it, in particular to devices for performing analyses of real time PCR. The detector of the present invention has a series of advantages such as drastic simplification of the detection configuration, reduced costs, better performances due to the greater freedom in planning the optical configuration which allows dividing the detector itself into independent areas.

A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.

A light sheet microscope includes an objective, an illumination optical system, a first adjustor, a second adjustor and a controller. The illumination optical system irradiates sample with a light sheet from a direction that is different from an optical axis direction of the objective. The first adjustor adjusts a relative position between a light sheet plane on which the light sheet is formed and the objective in an optical axis direction of the objective. The second adjustor adjusts a relative position between the light sheet plane and the sample in an optical axis direction of the objective. The controller controls the first adjustor on the basis of light that is from the light sheet plane and that is detected via the objective when a relative position between the light sheet plane and the sample is changed by the second adjustor.

An atomic interferometer and methods for measuring phase shifts in interference fringes using the same. The atomic interferometer has a laser beam traversing an ensemble of atoms along a first path and an optical components train with at least one alignment-insensitive beam routing element configured to reflect the laser beam along a second path that is anti-parallel with respect to the first laser beam path. Any excursion from parallelism of the second beam path with respect to the first is rigorously independent of variation of the first laser beam path in yaw parallel to an underlying plane.

An assay device is disclosed comprising a housing and a test portion, electronic circuitry and an optical assembly each a least partially located in the housing. The test portion comprises one or more test zones adapted to receive an analyte and a fluorescent label associated with the analyte, the fluorescent label being excitable by excitation light and adapted to emit emission light upon excitation by excitation light. The electronic circuitry comprises one or more light sources and one or more light detectors. The optical assembly comprises one or more excitation light guides adapted to guide excitation light from the one or more light sources to the one or more test zones, and/or one or more emission light guides adapted to guide emission light from the one or more test zone to the one or more light detectors.

A scanning luminescence light microscope for spatial high resolution imaging a structure marked with a luminescent marker comprises a light source for luminescence inhibition light and for further light; a light shaping and aligning device; and a detector registering luminescence light emitted by the luminescent marker. The device, by means of two optical gratings and an objective lens, forms two crossing line gratings of the luminescence inhibition light, and two crossing line gratings of the further light so that local intensity minima of an overall intensity distribution of the luminescence inhibition light are delimited in at least two directions, and that local intensity maxima or local intensity minima of an overall intensity distribution of the further light coincide with the local intensity minima of the luminescence inhibition light. Further, the device moves the overall intensity distributions of the further light and the luminescence inhibition light to scan the structure.

Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.