Disclosed are nanostructured gold films which may be produced by solution-phase depositions of gold ions onto a variety of surfaces. The resulting plasmonic gold films are used for enhanced spectroscopic-based immunoassays in multiplexed microarray format with detection mechanisms based on either surface-enhanced Raman scattering or near-infrared fluorescence enhancement. The preparation of the films and subsequent modifications of the gold film surfaces afford increased sensitivity for various microarrays. The films are discontinuous, forming gold “islands.” Sensitivity, size, shape, and density of the nanoscopic gold islands comprising the discontinuous nanostructured gold film are controlled to enhance the intensity of Raman scattering and fluorescence in the near-infrared, allowing for improved measurements in clinical diagnostic or biomedical research applications.

The present disclosure is directed to systems for tuning nanocube plasmonic resonators and methods for forming tunable plasmonic resonators. A tunable plasmonic resonator system can include a substrate and a nanostructure positioned on a surface of the substrate. The substrate can include a semiconductor material having a carrier density distribution. A junction can be formed between the nanostructure and the substrate forming a Schottky junction. Changing the carrier density distribution of the semiconductor material can change a plasmonic response of the plasmonic resonator.

Analyte collection and testing systems and methods, and more particularly to testing systems and methods that achieve significant improvements in the detection of fluorescence signals in the reader by modulating the applied optical excitation. Also described herein are optical detection apparatuses and methods for removable photonic chips that do not require translation for calibration when coupling the photonics chip with the sensing system. Also described herein are methods and apparatuses for accurately calibrating a dilution factor when reading from a photonics chip.

A prism (1090) is configured from a dielectric medium and is used in analysis using surface plasmons. The prism (1090) is provided with an incidence surface (1170) on which excitation light from outside is incident, a reflection surface (1172) on which excitation light having entered the incidence surface (1170) is reflected, an emission surface (1174) from which excitation light reflected by the reflection surface (1172) is emitted, and an opposing surface (1175) opposing the reflection surface (1172). A gold film (1092) is formed on the reflection surface (1172). The opposing surface (1175) has a sink-mark surface (1200), and the sink-mark surface (1200) is a transparent surface.

Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).

The invention relates to a carrier with a binding surface at which target components that comprise label particles, for example magnetic particles, can collect and optionally bind to specific capture elements. An input light beam (L1) is transmitted into the carrier and totally internally reflected at the binding surface. The amount of light in the output light beam (L2) and optionally also of fluorescence light emitted by target components at the binding surface is then detected by a light detector. Evanescent light generated during the total internal reflection is affected (absorbed, scattered) by target components and/or label particles at the binding surface and will therefore be missing in the output light beam (L2). This can be used to determine the amount of target components at the binding surface from the amount of light in the output light beam (L2, L2a, L2b). A magnetic field generator is optionally used to generate a magnetic field (B) at the binding surface by which magnetic label particles can be manipulated, for example attracted or repelled.

The object of the present invention is to provide a novel light enhancement device manufactured easily. According to the present invention, there is provided a light enhancement device including a substrate provided with an adsorption layer formed on its surface and including a hydrophobic modified clay with organic compound; and tabular silver nanoparticles oriented and adsorbed on the adsorption layer. Furthermore, there is provided a manufacturing method of a light enhancement device including a step of forming an adsorption layer including a hydrophobic modified clay with organic compound on a surface of a substrate; and a step of immersing the substrate in an aqueous dispersion of tabular silver nanoparticles. Furthermore, there is provided a kit for spectroscopic analysis including a substrate provided with an adsorption layer including a hydrophobic modified clay with organic compound formed on its surface; and a container filled with an aqueous dispersion of tabular silver nanoparticles.

In one embodiment, the present invention relates to a method for establishing a solvent correction curve as well as using the curve for obtaining a corrected sensorgram or corrected report points from a sensorgram of an analyte. In another embodiment, the present invention provides an analytical system for studying molecular interactions, which comprises computer processing means including program code means for performing the steps of the methods. Also provided is a computer program product comprising program code means stored on a computer readable medium or carried on an electrical or optical signal for performing the steps of the methods.

A DNA ligand capable of structural changes upon binding to a target is used as a molecular switch with a SPFS (surface plasmon field-enhanced fluorescence spectroscopy) biosensor to realize one-step SPFS biosensing with rapid turnaround time. The SPFS biosensor has a thin metal film on a prism; when a light of a certain wavelength irradiates on the prism at a certain angle, a strong electrical field is generated at the surface of the metal film. The DNA is immobilized on the metal film surface with its free terminal modified with a fluorescent marker. Without the target, the DNA is folded and the fluorescent marker is located in the region of metal quenching near the metal surface. Upon binding to the target, the DNA is extended and the fluorescent marker is located in the region of enhanced electric field near the metal surface and emits a strong fluorescent signal.

A device includes a substrate, a pedestal extending from the substrate, and a ring resonator disposed on the pedestal above the substrate. The ring resonator has a resonance wavelength greater than 1.5 μm and includes at least one of silicon and chalcogenide glass. The device can be used as a ring resonator sensor or a light source. The ring resonator is substantially transparent to mid-infrared radiation to reduce optical losses. The pedestal has a narrower width compared to the ring resonator to generate improved interaction between evanescent fields of light in the ring resonator and analytes nearby the ring resonator, thereby increasing sensing sensitivity. In addition, fabrication of the device is compatible with complementary metal-oxide-semiconductor (CMOS) processes and hence is amenable to large scale manufacturing.