A method for detecting an analyte includes first to third steps. The first step is distributing a sample containing a bead modified with a host molecule that is specifically bound to the analyte in a microchannel using a syringe pump. The second step is irradiating the sample with non-resonant light that is light outside a wavelength range of electronic resonance of the bead. The third step is detecting the analyte based on a signal from a camera that receives the light from the sample.

A detection method uses a reaction vessel including a container including a housing part having a first opening opened at an upper portion and a second opening opened at a side portion, and a side wall member fixed to the container so that a capturing region on a metal film is exposed in the second opening. First, a liquid containing a specimen is provided to the housing part. Next, the liquid in the housing part is stirred to capture, into the capturing region, a substance to be detected in the liquid. Next, the metal film is irradiated with light so that surface plasmon resonance occurs in the metal film, and light emitted from the reaction vessel and having a light amount changing depending on the amount of the substance to be detected captured in the capturing region is detected. In the step of providing the liquid to the housing part, an amount of liquid in which the liquid is not in contact with the capturing region when the liquid in the housing part is left still, and the liquid is in contact with the capturing region when the liquid in the housing part is stirred is provided to the housing part.

A microfluidic device includes a substrate having an electromagnetic wave transmission property, a lid member facing the substrate and being separated from the substrate such that a flow channel is formed between the substrate and the lid member, a light absorption layer which is placed in the flow channel and absorbs an electromagnetic wave, and a microwell array formed on the substrate and having plural microwells that are open to the flow channel to receive a target of analysis.

In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In yet another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas with different angles of illumination during a sequence of sampling events, including, for a sampling event, illuminating each of the wells with off-axis illumination to produce asymmetrically illuminated well regions in each of the wells.

A system for analysis of a sample at a substrate comprises: a light source to generate first light; and a spatial light modulator to form second light from the first light, wherein the substrate includes at least one sensor to detect an emission emitted based on the second light, wherein at the substrate the second light forms a shape selected based on the at least one sensor, wherein the second light illuminates an area of the substrate corresponding to the shape.

Disclosed is an apparatus and method of forming, including a supporting structure, a sensor on the supporting structure, a pair of columns on the supporting structure at opposite sides of the sensor, the pair of columns having a column height relative to a top surface of the supporting structure, the column height being higher than a height of the active surface of the sensor relative to the top surface of the supporting structure, and a lidding layer on the pair of columns and over the active surface, the lidding layer being supported at opposite ends by the pair of columns. The active surface of the sensor, the lidding layer and the pair of columns form an opening above at least more than about half of the active surface of the sensor, and the supporting structure, the sensor, the lidding layer and the pair of columns together form a flow cell.

A micropump for exchanging liquid between a supply region and a working region is provided. An enclosed gas region is located above the working region. The micropump includes a capillary pipette having a closed pipette tip on a first end, an open pipette inlet disposed opposite the first end, and a pipette section enclosing the working region and disposed in a direction of the open pipette inlet from the closed pipette tip. The micropump further includes a liquid-permeable filter covering the open pipette inlet and connected to the supply region. The micropump additionally includes a capillary structure extending through the gas region between the closed pipette tip and the liquid-permeable filter.

A method for providing quantitative information for targets and a device using the same according to an exemplary embodiment of the present disclosure are provided. A quantitative information providing method for targets according to the exemplary embodiment of the present disclosure includes flowing a plurality of microdroplets into a chamber or a channel including a detection region acquiring a single layer of microdroplets in which the plurality of microdroplets is present as a single layer, and providing quantitative data of targets based on the single layer image of the microdroplets, and the detection region has a height which is one time to about two times of a diameter of the plurality of microdroplets and is defined as a region in which the plurality of microdroplets is dispersed in a plurality of columns to fill the detection region.

Techniques are described for reducing the number of angles needed in structured illumination imaging of biological samples through the use of patterned flowcells, where nanowells of the patterned flowcells are arranged in, e.g., a square array, or an asymmetrical array. Accordingly, the number of images needed to resolve details of the biological samples is reduced. Techniques are also described for combining structured illumination imaging with line scanning using the patterned flowcells.

A system (100) and a method for detecting fluorescence is disclosed. The system (100) essentially comprises a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, a chamber for holding said labelled sample during said LASER irradiation, a reflective layer (108) positioned to reflect said electromagnetic radiation, and a detector (112) positioned to detect and amplify said electromagnetic radiation. The method essentially comprises the steps of providing a labelled sample wherein said labelled sample emits an electromagnetic radiation of a defined wavelength when irradiated by a LASER beam of a commensurate wavelength, providing a source (102) for emitting said LASER beam, oriented as to aim at said labelled sample, providing a chamber for holding said labelled sample during said LASER irradiation, providing a reflective layer (108) positioned to reflect said electromagnetic radiation, providing a detector (112) positioned to detect and amplify said electromagnetic radiation, irradiating said sample with said LASER beam and analyzing said amplified electromagnetic radiation from said detector (112) with a signal processing block (114).