A removal method of a carbon dioxide includes: loading a sample into the ion chromatography system; combining the sample with a liquid mobile phase to be an object under test, wherein the object under test includes a plurality of target anions and a plurality of cations; separating the target anions in the object under test into a plurality of groups; replacing the cations in the object under test with a plurality of hydrogen ions; creating a vacuum environment; transmitting the object under test to the vacuum environment; and removing a plurality of carbon dioxide molecules, a plurality of carbonic acid molecules, a plurality of bicarbonate ions, or a plurality of carbonate ions in the object under test to make a concentration of the carbonate ions and a concentration of the bicarbonate ions be equal to or less than about one part per billion.

A system and method is provided for validating the manufacturing process for the production of complex biological compositions, and particularly for providing process validation information for evaluation by a federal regulatory agency. The system and method continuously assess the concentration of proteoforms within the biological composition as it is being produced in a fermentor. Samples from the fermentor are analyzed in a pre-selected array of analysis columns, with data generated by the columns being accumulated and evaluated, and particularly compared with data from previous stages in the production process. A continuous process validation system includes top-down and bottom-up analysis sectors, each including a plurality of different analysis columns that can be selected by the controller for a particular biological composition and a particular production process.