A microfluidic check valve includes an inlet bore, an internal chamber, an outlet bore, and a disk freely movable in the chamber between an open position and a closed position. At the open position, the disk permits fluid to flow from the inlet bore, through the chamber, and to the outlet bore. At the closed position, the disk prevents fluid from flowing in the reverse direction from the chamber into the inlet bore. The check valve may be positioned in-line with a fluid conduit, and/or incorporated with various fluidic devices such as, for example, capillary tubes, fittings, and chromatography columns. The check valve is capable of withstanding high fluid pressures, while featuring a small swept volume, such as a nano-scale volume. The check valve may be utilized, for example, to prevent fluid back flow and isolate pressure pulses in fluid flow systems.

An injection valve switches between a first state in which a sample loop is connected to a separation flow path through which a mobile phase from a liquid sender flows and a second state in which the sample loop is disconnected from the separation flow path. A pump speed determiner, in a case where a sample intake operation by a syringe pump is started immediately after the injection valve is switched from the second state to the first state, and a filling operation of filling the sample loop with the sample by the syringe pump is started after the intake operation is completed, determines an intake operation speed of the syringe pump that is required in order for the filling operation to complete immediately before the injection valve is switched from the second state to the first state next time using a set injection interval time. The syringe pump operates at the intake operation speed determined by the pump speed determiner while performing the intake operation. The sample is injected by the injector at intervals of the set injection interval time.

Tests of channels of a system as a whole can be easily performed without adding a complicated mechanism. A liquid feeding part includes a liquid feeding channel to feed a mobile phase, a drainage channel to release pressure in the liquid feeding channel, an analysis channel that discharges the mobile phase into the sample introduction part, and a channel switching valve that selectively connects the liquid feeding channel to one of the drainage channel and the analysis channel. The channel switching valve is configured to be able to provide a tight stopper state in which the liquid feeding channel is connected to neither the analysis channel nor the drainage channel.

An analyzing device includes a splitting part for causing fluid containing a sample component to flow separately in a first flow passage and a second flow passage; an analyzing column provided on the first flow passage for separating the sample component from the fluid; a first back pressure regulating valve corresponding to a first pressure controlling unit for controlling a pressure in the first flow passage; and a second back pressure regulating valve corresponding to a second pressure controlling unit for controlling a pressure in the second flow passage, wherein flow rate of the fluid in the first flow passage and flow rate of the fluid in the second flow passage are controlled based on a ratio of the pressure in the first flow passage to the pressure in the second flow passage.

The disclosure relates to a gradient twin column recycling chromatography method that is used to separate a mixture containing closely eluting compounds. In one embodiment, a sample includes a primary organic compound and one or more impurities that closely elute with the primary organic compound. A gradient mobile phase is initially used to remove unwanted early eluting and late eluting impurities from the sample. After the gradient removal of some of the impurities is complete, the remaining mixture of the primary organic compound and the closely eluting impurities are separated using recycle chromatography methodology with an isocratic mobile phase.

There is provided a mass spectrometer that can appropriately maintain the atmospheric pressure of a vacuum chamber, and a method of controlling the same. An example of a mass spectrometer according to the present invention includes first vacuum chambers, first vacuum pumps, an atmospheric pressure relating value acquiring unit, and an adjustment unit configured to adjust the effective exhaust velocity of the first vacuum pumps, and controllers. The controllers control the adjustment unit corresponding to an atmospheric pressure relating value.

Exemplary embodiments are directed to vent valves, systems and methods generally involving a valve body that includes a seat retainer, a needle and a seat. The seat includes a bore extending there through and the needle includes a needle stem and a needle head. The seat is disposed inside the seat retainer. The needle stem is disposed inside the bore. The needle is configured to be pulled through the seat to stop flow through the bore. Exemplary embodiments are further directed to a system including a stem return spring mechanism and a solenoid return spring mechanism. A processing device is configured to actuate the solenoid return spring mechanism to permit the stem return spring mechanism to pull the needle through the seat to stop flow through the bore.

The present invention relates to a valve assembly, comprising a valve chamber, accesses to the valve chamber, the accesses including a first access and a second access, and a movable sealing body assembly comprising at least one sealing portion, wherein at least a portion of the sealing body assembly is magnetic, and wherein at least a portion of the sealing body assembly comprising the at least one sealing portion is located within the valve chamber. Further, the valve assembly comprises at least one sealing surface, wherein each of the at least one sealing surface is configured to complement one of the at least one sealing portion, and wherein each sealing surface comprises an orifice fluidly connected to one of the accesses. The valve assembly further comprises a force unit configured to exert a magnetic force on the magnetic portion of the movable sealing body assembly and is configured to assume at least two configurations, wherein in a first configuration, the first access is sealed, and wherein in a second configuration, the first access is fluidly connected to the second access. Further, the present invention relates to a pump system, as well as a use and manufacturing method of a valve assembly according got the present invention.

A back-pressure control valve includes a main body having an inner space, a valve element that is arranged in the inner space of the main body and has an opposing surface opposite to one surface of the inner space, a driver that moves the valve element such that a distance between the opposing surface of the valve element and the one surface in the inner space changes; and a resin coating formed on one of the one surface in the inner space and the opposing surface of the valve element, wherein the main body has a first flow path that guides a fluid to a pressure control space formed between another surface out of the one surface and the opposing surface of the valve element, and the resin coating, and a second flow path that discharges a fluid from the pressure control space.

A target component is collected using a preparative separation and purification device having a holder for holding a trap column in which the target component has been captured, a liquid feeder for feeding a first solvent having compatibility with the water remaining in the trap column and a second solvent having low compatibility with water and high compatibility with the first solvent into the trap column, a flow-path switch for connecting the exit end of the trap column to a waste liquid flow path and a collection flow path, and a control unit for controlling the flow-path switch so that solution including water flows into the waste liquid flow path.