A fluid supply system (150) adapted for metering two or more fluids in controlled proportions and for supplying a resultant mixture, the fluid supply system (150) comprising a plurality of solvent supply lines (104 to 107), each fluidically connected with a fluid source (100 to 103) providing a respective fluid, a pumping unit (110) comprising a reciprocating element (115) adapted for intaking fluid supplied at an inlet of the pumping unit (110) and for supplying the pressurized fluid at an outlet of the pumping unit (110), wherein the pumping unit (110) is adapted for taking in fluids from selected solvent supply lines (104 to 107) and for supplying a pressurized mixture of the fluids at its outlet, a proportioning valve (108) interposed between the solvent supply lines (104 to 107) and the inlet of the pumping unit (110), the proportioning valve (108) adapted for modulating solvent composition by sequentially coupling selected ones of the solvent supply lines (104 to 107) with the inlet of the pumping unit (110), and a longitudinal mixing unit (152) adapted for mixing longitudinally subsequent sections of the fluids so as to modify their succession in flow direction.

The invention discloses a method for predicting the solubility of at least one species at a specified pH value in an aqueous buffer comprising at least one weak acid species and/or at least one weak base species. The method comprises the steps of: a) selecting a start composition of the buffer, giving a start value for the total solute concentration; b) calculating the concentrations of all ionic species present in the buffer at the specified pH value from the total composition of the buffer and available dissociation constants; c) calculating the solubility limits of each combination of ionic species present in the buffer from available solubility products, taking the concentrations calculated in step a) into account; d) comparing the concentrations of all ionic species calculated in step a) with the solubility limits calculated in step b) and determining if any solubility limit is exceeded; e) if no solubility limit is exceeded, increasing the total solute concentration of the buffer or, if at least one solubility limit is exceeded, decreasing the total solute concentration of the buffer, and; f) repeating steps b)-e) until a predetermined convergence criteria is met.

A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.

In the present system and method, a conduit from a LC device continuously transports solvent, buffers, and analytes to the inlet of a solvent removal and analyte conversion device which evaporates the solvents, leaving non-volatile analytes for detection. The device comprises a rotating disk. The liquid chromatograph device can be any device using liquid chromatography to separate molecules. The solvents in the LC effluent can include, but are not limited to, water, methanol, acetonitrile, tetrahydrofuran, and acetone. After removal of the volatile components, the non-volatile analytes are converted with a concentrated energy source so that they may be detectable.

A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more solvents and water, and the method comprises the steps of: calculating pH and/or solvent concentration at a particular time t from a user-determined gradient function; and, based on the values obtained, calculating percent acid, percent base, percent solvent and percent water in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided.

An aqueous solution including salt stored in a first storage is led to a mixer through a first pipe, and an organic solvent stored in a second storage is led to the mixer through a second pipe. The aqueous solution and the organic solvent are mixed by the mixer, so that a mobile phase is produced. At least a portion of the first pipe and at least a portion of the second pipe are heated by a heater such that a temperature of the mobile phase produced by the mixer is equal to or higher than the dissolution temperature of salt included in the aqueous solution.

The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a metering device for drawing up the liquid sample, a first multi-port valve in fluid communication with a first end of the metering device and the liquid sample, a second multi-port valve in fluid communication with a second end of the metering device and a chromatography column, and a pump in fluid communication with the second multi-port valve and a mobile phase. When the valves are in a first position the metering device draws up the liquid sample filling a portion of the metering device. When the valves are in a second position, a remaining portion of the metering device is filled with the mobile phase thereby mixing with and pressurizing the liquid sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

A liquid chromatograph includes a liquid sender (2) that sends a plurality of solvents, a mixer (6) that has an internal capacity for mixing the plurality of solvents sent by the liquid sender (2), a sample injector (10) that injects a sample into an analysis flow path (8) through which the solvents mixed in the mixer (6) flow as a mobile phase, a separation column (12) provided on the analysis flow path (10) for separating the sample injected by the sample injector (10) to each component, a detector (14) provided downstream of the separation column (12) on the analysis flow path (8) for detecting the components of the sample separated in the separation column (12), a capacity information storage medium (16) that is attached to the mixer (6) to store information as to the internal capacity of the mixer (6), and a capacity specifier (18) configured to read the information stored in the capacity information storage medium (16) to specify the internal capacity of the mixer (6).

A mixer system for a liquid chromatography system has a mixer block, a plurality of fluid inlets which are connected to a mixing chamber by a respective channel, and a fluid outlet for mixed fluid. Each channel has a valve assigned thereto which is adapted to control the fluid flow. An adapter which is attached to the mixer block and through which one of the channels passes is provided for each valve. The valve is attached to the appropriate adapter, and the valves are arranged in an annular shape around the mixer block.

A fluid mixer includes a flow splitter and a mixing chamber. The flow splitter includes an inlet for receiving a flow of fluid and is configured to split the flow of fluid into first and second fluid streams. The second fluid stream has a higher density than the first fluid stream. The mixing chamber includes a first inlet, a second inlet and a mixing well. The second inlet is positioned below the first inlet. The second inlet of the mixing chamber is configured to receive the first fluid stream and the first inlet of the mixing chamber is configured to receive the second fluid stream to promote mixing of the first and second streams in the mixing well.