A sample dispatcher for a fluid separation apparatus includes a sampling path including a sampling volume, a sampling unit, and a retaining unit. The sampling unit receives a fluidic sample, and the sampling volume temporarily stores an amount of the received sample. The retaining unit receives and retains from the sampling volume at least a portion of the stored sample, and has different retention characteristics for different components of the sample. A switching unit is coupled to the sampling path, a sampling fluid drive, a mobile phase drive, and a separating device. In a feed injection configuration of the switching unit, the mobile phase drive, the separating device, and the sampling path are coupled together in a coupling point for combining a flow from the sampling fluid drive containing the fluidic sample retained by the retaining unit with a flow of the mobile phase from the mobile phase drive.

A liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream, the fluidic stream comprising a sample-injection valve, a trap-bypass-selection valve, a column-bypass valve, a load-elute valve and a trap-selection valve. Also, a liquid chromatographic (LC) system is introduced which comprises at least one fluidic stream. The fluidic stream comprises a first substream and a second substream. The first substream comprises a first sample-injection valve, a load-elute valve and a trap-selection valve. The second substream comprises a second sample-injection valve and a column-bypass valve. The fluidic stream further comprises a trap-LC substream transfer valve and a substream-selection valve. The LC systems provide a broad choice of chromatographic options and modes and enable to flexibly and rapidly switch between them.

A method for determining a state of a fluidic system can include measuring back pressures in the fluidic system at different times and determining a state of the fluidic system. The determination is based on at least the measured back pressures and on additional status information indicative of at least one status of the fluidic system at at least one of the different times.

The present invention relates to a method for the preparation of nanoscale nucleic acid-encircled lipid bilayers, the nanoscale nucleic acid-encircled lipid bilayers and their use.

The present invention relates to an improvement in a technique of a large-volume sample injection in high-performance liquid chromatography or supercritical fluid chromatography.

A sample injection device 10 comprises: a sample container 12; a sample loop 14; a syringe 16, a liquid transfer channel 18; a first channel switching valve 30 that switches the sample loop to be detachable to the liquid transfer channel; and a second channel switching valve 40 that switches the channel of the syringe 16 together with the first channel switching valve.

The second channel switching valve 40 comprises a stator part that has six through holes at vertex positions of a regular octagon, and a rotor part that rotates in contact with the stator part to switch a channel.

The rotor part has three channel grooves, and two of the through holes and one of the channel grooves face each other to form a channel.

An analysis device of the present invention is provided with a sample introduction unit that introduces a sample into a mass spectroscope; a sample condensation unit that treats the sample introduced into the device; a detection unit that analyzes the sample treated by a treatment unit; and a control unit that controls the sample introduction unit, the sample condensation unit, and the detection unit. The sample introduction unit includes a sample introduction valve, and the sample condensation unit includes an elution valve and a cleaning valve, and the cleaning valve is disposed between the sample introduction valve and the elution valve.

The present invention relates to a method for collecting a sample for sample analysis. The method comprises a system assuming a first configuration and drawing a first portion of the sample into a sample storage portion of the system in the first configuration; the system assuming a second configuration, which is different from the first configuration, and preparing the system in the second configuration to draw a second portion of the sample into the sample storage portion; the system assuming a third configuration and drawing the second portion of the sample into the sample storage portion in the third configuration; and the system assuming an injection configuration, wherein the sample storage portion is fluidly connected to a chromatography column, and supplying the first portion of the sample and the second portion of the sample from the sample storage portion to the chromatography column, while the system is in the injection configuration. The present invention also relates to a corresponding system and a corresponding use.

A microfluidic device for separating a phase in a specimen has been described. This is based on a microfluidic trapping area, channels connected to it and integrated inputs and outputs connected onto the channels. An additional integrated input is provided which allows the flow in the device to be controlled and which may prevent leaking of the specimen and the phase.

A liquid chromatography system includes a separation column, a trap column, and a first switching valve. The first switching valve is adapted to assume a first switching position for bringing a sample into the trap column in a first flow direction. The switching valve is also adapted to assume a second switching position for fluidly connecting the trap column with the separation column and providing a flow from the trap column to the separation column in a second flow direction. The second flow direction is opposite to the first flow direction. The first switching valve is adapted to assume a third switching position for fluidly connecting the trap column, with the separation column and providing a flow from the trap column to the separation column in the first flow direction.

An alternating flow column chromatography apparatus comprising a U shaped or T shaped separation column including at least one loading port for loading of components for separation, a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column, at least one eluent input port, an eluate output port and an alternating flow valve in fluid communication with the primary eluent input port, the eluate output port, the first purification column and the second purification column wherein, when operated, the alternating flow valve reverses the flow of eluent through the purification columns and the separation column. Also a method of using the apparatus. A benefit of the apparatus and method is more efficient operation compared to existing direct flow column chromatography apparatuses.