The present disclosure is related to an analytical system comprising a liquid chromatographic (LC) system comprising a plurality of fluidic streams alternately connectable to a common detector via a stream-selection valve connected to the detector via a valve-to-detector conduit. The analytical system further comprises a wash pump fluidically connected to the stream-selection valve and configured to connect to the valve-to-detector conduit between two consecutive fluidic streams in order to wash liquid from a previous fluidic stream out of the valve-to-detector conduit before liquid from a subsequent fluidic stream enters the valve-to-detector conduit. An analytical method comprising switching between the fluidic streams and washing in between is also disclosed.

A method for collecting a sample for sample analysis includes drawing a first portion of the sample into a sample storage portion of a chromatography system while the chromatography system is in a first configuration. The method further comprising switching the chromatography system to a second configuration; sealing an end of a sample pick-up needle and draining a portion of a liquid from the second tubing; switching the chromatography system to a third configuration; drawing a second portion of the sample into the sample storage portion of the chromatography system; switching the chromatography system to an injection configuration; and fluidly connecting the sample storage portion to the chromatography column and supplying the first portion of the sample and the second portion of the sample from the sample storage portion to the chromatography column.

Examples of the present disclosure describe systems and methods for detecting and mitigating stack pivoting exploits. In aspects, various “checkpoints” may be identified in software code. At each checkpoint, the current stack pointer, stack base, and stack limit for each mode of execution may be obtained. The current stack pointer for each mode of execution may be evaluated to determine whether the stack pointer falls within a stack range between the stack base and the stack limit of the respective mode of execution. When the stack pointer is determined to be outside of the expected stack range, a stack pivot exploit is detected and one or more remedial actions may be automatically performed.

A liquid chromatograph includes a sample supplier that supplies a sample into a mobile phase, a chip column having a flow path that functions as a separation column on a substrate, a packed column, a switcher configured to be switchable between a first state in which a mobile phase and a sample are guided from the sample supplier to the chip column and a second state in which a mobile phase and a sample are guided from the sample supplier to the packed column, and a detector that detects a sample that has passed through the chip column and a sample that has passed through the packed column.

Field flow fractionation device includes a channel switching unit for switching the connection of a second carrier fluid supply unit to any one of the second inlet port of an upper separation cell, the first inlet port of a lower separation cell, or the second inlet port of a lower separation cell. Furthermore, the second carrier fluid supply unit is connected to the second inlet port of an upper separation cell during the process of focusing to generate flow of carrier fluid counter to the flow of carrier fluid from the first inlet port within the upper separation cell, whereas the second carrier fluid supply unit is connected to the first inlet port or the second inlet port of a lower separation cell after conclusion of focusing in the upper separation cell.

Field flow fractionation device includes a channel switching unit for switching the connection of a second carrier fluid supply unit to any one of the second inlet port of an upper separation cell, the first inlet port of a lower separation cell, or the second inlet port of a lower separation cell. Furthermore, the second carrier fluid supply unit is connected to the second inlet port of an upper separation cell during the process of focusing to generate flow of carrier fluid counter to the flow of carrier fluid from the first inlet port within the upper separation cell, whereas the second carrier fluid supply unit is connected to the first inlet port or the second inlet port of a lower separation cell after conclusion of focusing in the upper separation cell.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication

Go between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

A liquid chromatograph analysis method and a liquid chromatograph minimize analysis time when performing analyses by switching columns and mobile phases. Liquid chromatograph 100 for performing a plurality of analyses according to a schedule table includes: mobile phase switching sections 15, 16 for switching a plurality of mobile phases to select a mobile phase to be used in analysis; column switching sections 31, 33 for switching a plurality of columns 32a-32f to select a column to be used in analysis; and control section 60 including memory 61 for storing an equilibration time of each of the plurality of columns 32a to 32f and an equilibration controller 66 for controlling column equilibration. If used columns or used mobile phases are different between two consecutively executed analyses, equilibration controller 66 equilibrates a column used in the later of the two analyses over an equilibration time read out from memory 61.

Exemplary embodiments are directed to devices, methods and systems capable of pressurization, generally involving a flow system that includes a pressurized reservoir, at least one pump including a pump control valve, an outlet port, a shut-off valve and a vent valve. The flow system is configured to be pressurized. The shut-off valve is disposed between the pressurized reservoir and the at least one pump. The vent valve is disposed between the at least one pump and the outlet port. The shut-off valve, the vent valve and the pump control valve of the at least one pump are configured to actuate in a coordinated manner to control a pressurization of the flow system. Exemplary embodiments are further directed to devices, methods and systems for column switching, generally including at least a first column, a second column and a column switching valve.

The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).