A range of pH of a mobile phase that is usable for an analysis with use of each column is registered by a pH range registrar in association with identification information of each column. A pH of each mobile phase is registered by a pH registrar. A column to be used for an analysis is selected by a column selector. A chromatograph is controlled by an analysis controller such that a mobile phase having a registered pH that is out of a range of pH that is registered in association with a selected column is not supplied to the selected column. The chromatograph is controlled by the analysis controller such that an analysis is performed while a mobile phase having a registered pH that is in a range of pH that is registered in association with a selected column is supplied to the selected column.

Field flow fractionation device includes a channel switching unit for switching the connection of a second carrier fluid supply unit to any one of the second inlet port of an upper separation cell, the first inlet port of a lower separation cell, or the second inlet port of a lower separation cell. Furthermore, the second carrier fluid supply unit is connected to the second inlet port of an upper separation cell during the process of focusing to generate flow of carrier fluid counter to the flow of carrier fluid from the first inlet port within the upper separation cell, whereas the second carrier fluid supply unit is connected to the first inlet port or the second inlet port of a lower separation cell after conclusion of focusing in the upper separation cell.

Field flow fractionation device includes a channel switching unit for switching the connection of a second carrier fluid supply unit to any one of the second inlet port of an upper separation cell, the first inlet port of a lower separation cell, or the second inlet port of a lower separation cell. Furthermore, the second carrier fluid supply unit is connected to the second inlet port of an upper separation cell during the process of focusing to generate flow of carrier fluid counter to the flow of carrier fluid from the first inlet port within the upper separation cell, whereas the second carrier fluid supply unit is connected to the first inlet port or the second inlet port of a lower separation cell after conclusion of focusing in the upper separation cell.

The present invention relates to method of using spectroscopy for real time measuring of concentration of desired product and using measured data for monitoring and control of chromatography. It develops a method and system for measuring real-time concentration of clarified harvest and that of flow through of loading step of the chromatography and using measured data for determining breakthrough in real-time. The two modes of operation are used viz. first mode (Part A) uses a single near infrared spectroscopy (NIR) flow cell prior to the continuous chromatography column to ensure optimal loading in each cycle based on dynamic binding capacity studies carried out previously with the desired Protein A resin and second mode (Part B) uses two near infrared spectroscopy (NIR) flow cells, one before and one after the column, to detect the breakthrough curve (from 1% breakthrough onwards).

Certain embodiments described herein are directed to chromatography systems that include a microfluidic device. The microfluidic device can be fluidically coupled to a switching valve to provide for selective control of fluid flow in the chromatography system. In some examples, the microfluidic device may include a charging chamber, a bypass restrictor or other features that can provide for added control of the fluid flow in the system. Methods of using the devices and methods of calculating lengths and diameters to provide a desired flow rate are also described.

In the present invention, an analysis schedule is pre-created such that streams of a plurality of liquid chromatograms can operate in parallel and a mass spectrometer can collect data at the timing of each component elution. A control unit controls so as to: divide the time required to analyze each sample in a plurality of liquid chromatogram systems into pre-collection time, time during collection, and post-collection time; search and allocate time positions in which the time during collection in the liquid chromatogram units do not overlap; determine start times for the plurality of liquid chromatogram units to thereby create an analysis schedule; and thereafter perform analysis. The control unit further stores parameter sets for varying component elution times, adjusts analysis parameters so as to make data collection timings appropriate for creating an analysis schedule, and changes the component elution times.

An autosampler for a chromatograph includes a first injection port through which a sample is injected into a first analysis flow path of the chromatograph, a second injection port through which a sample is injected into a second analysis flow path of the chromatograph, a needle that is movable to both of the first injection port and the second injection port, and injects a sample into the first injection port and the second injection port, a first sample loop that stores a sample to be injected into the first analysis flow path, a second sample loop that stores a sample to be injected into the second analysis flow path, and a metering pump that loads a sample in the first sample loop and the second sample loop.

A liquid chromatography-mass spectrometry (LC-MS) apparatus including an ionization source coupled to a mass spectrometer and a liquid chromatographic (LC) system coupled to the ionization source. The LC system comprises multiple fluidic streams alternately connectable to the ionization source, thereby assigning a detection time window to each fluidic stream from the multiple fluidic streams when connected to the ionization source. The LC-MS apparatus further comprises a controller configured to carry out steps of monitoring an ionization current of the ionization source for the multiple fluidic streams and identifying differences in flow conditions between the multiple fluidic streams based on the monitored ionization current. The controller is further configured to carry out adjusting detection conditions of one or more of the multiple fluidic streams responsive to the identified differences, thereby enabling eluates of interest from each fluidic stream to be detected by the mass spectrometer in the respective detection time window.

A separation system may include a number of parallel fluid paths. Each parallel fluid path may include a separation module, and an adjustable flow restrictor. Each adjustable flow restrictor is operable sequentially and operable such that the hydraulic resistance of all the parallel fluid paths is substantially the same and is equal to or higher than the hydraulic resistance of a fluid path identified to have the highest hydraulic resistance. The system includes a pressure sensor that measures pressure loss over the whole separation system. The system is operable such that the hydraulic resistances of the respective separation modules are synchronised, and such that when operated in parallel and at substantially the same time, the respective modules have substantially the same time residence times. The system may include a control system for automated operation.

A separation system may include a number of parallel fluid paths. Each parallel fluid path may include a separation module, and an adjustable flow restrictor. Each adjustable flow restrictor is operable sequentially and operable such that the hydraulic resistance of all the parallel fluid paths is substantially the same and is equal to or higher than the hydraulic resistance of a fluid path identified to have the highest hydraulic resistance. The system includes a pressure sensor that measures pressure loss over the whole separation system. The system is operable such that the hydraulic resistances of the respective separation modules are synchronised, and such that when operated in parallel and at substantially the same time, the respective modules have substantially the same time residence times. The system may include a control system for automated operation.