Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

A method for operating a chromatography setup of a bioprocess installation with a plurality of chromatography columns, each with a column inlet and a column outlet, and a valve switching cassette with a group of inlet ports, a group of outlet ports, a group of column-in ports and a group of column-out ports . It is prosed, that a first liquid stream of concentrated buffer is introduced into a first internal liquid line via a first inlet port and that a second liquid stream of diluent is introduced into a second internal liquid line via a second inlet port, that in a dilution process, the array of valve units is switched as to create a third liquid stream by merging the first liquid stream and the second liquid stream at a merging location within the valve switching cassette.

Disclosed is a device for chromatographic separations comprising: a manifold comprising a manifold body defining an elongate central duct, the central duct comprising a centrally-located closable duct valve providing selective fluid communication between a first portion of the central duct and an opposed second portion of the central duct, a first plurality of connectors, each connector of the first plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; a second plurality of connectors, each connector of the second plurality of connectors for connecting to a distinct chromatographic separation column and/or feed or extraction tubing or to a connector of an adjacent manifold; wherein said manifold body further defines: a first plurality of branch ducts, each branch duct of which extending from the first portion of the central duct to an individual one of the first plurality of connectors, each of the branch ducts of the first plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the first portion of the central duct, a second plurality of branch ducts, each branch duct of which extending from the second portion of the central duct to an individual one of the second plurality of connectors, each of the branch ducts of the second plurality of branch ducts comprising a closable branch valve providing selectable fluid communication between a respective connector and the second portion of the central duct; first and second ports in fluid communication with the centrally-located closable duct valve wherein said first port communicates with said first portion of the central duct and said second port communicates with said second portion of said central duct, wherein one of said first and second ports is further positioned to communicate with said central duct at a location between the centrally-located closable duct valve and the first and second plurality of branch ducts, respectively.

The disclosure describes embodiments of an apparatus including a first gas chromatograph including a fluid inlet, a fluid outlet, and a first temperature control. A controller is coupled to the first temperature control and includes logic to apply a first temperature profile to the first temperature control to heat, cool, or both heat and cool the first gas chromatograph. Other embodiments are disclosed and claimed.

Multidimensional liquid chromatography (MDLC) systems include valve configurations that enable a flow containing analytes in the eluent of a first dimension liquid chromatography system to be modulated such that the analytes can be captured in fluidic loops and subsequently provided to a second dimension liquid chromatography system. Optionally, a single detector is used for both first and second dimensions. In addition, the systems can enable dilution of the analytes and enable incompatible mobile phases and mobile phase flow rates to be used in the first and second dimensions.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

The present invention relates to a novel analytical method for detecting one or more analytes in a source sample by continuous flow 2D LC-MS/MS using a single LC system.

Provided is a bioreactor arrangement for producing a biopolymer expressed by a cell and a continuous process for a capturing the biopolymer employing two chromatography units operated in series or independently.

The present invention relates to a bioreactor arrangement for producing a biopolymer expressed by a cell and a continuous process for a capturing the biopolymer employing two chromatography units operated in series or independently.

A system for component interconnection for use in liquid chromatography includes a first switching valve and a second switching valve. A first connecting line fluidly connects the first switching valve to the second switching valve. A second connecting line fluidly connects the first switching valve to the second switching valve. A metering device is located in the first connecting line.