Disclosed herein is a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

A sample is stored in a container (125) disposed upstream of a back-pressure control valve (140). A mixed fluid of carbon dioxide in a supercritical state and a modifier is introduced into the container, and a component contained in the sample is extracted. The extracted component is introduced into a trap column (135) together with the carbon dioxide and the modifier and collected in the trap column. The trap column is loaded with polymer beads as a filler.

A column packing material for supercritical fluid chromatography which allows good peak shape to be obtained in the analysis of free fatty acids, etc. and in the analysis of agricultural chemicals, etc., and has excellent durability for repeated analysis. The column packing material for supercritical fluid chromatography includes polymer particles containing cross-linked polymer, the degree of swelling of which after absorbing tetrahydrofuran and the degree of swelling of which after absorbing methanol are both 1.4 or less.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

A method and a system for transferring a slurry of a separation resin, from at least one first container to a second container, said method comprising the steps of: connecting the at least one first container to the second container by a transferring connection; providing a degree of vacuum to the second container by a vacuum production device connected to the second container; allowing the vacuum in the second container to affect the content in the at least one first container through the transferring connection such that the slurry initially provided in the at least one first container is transferred to the second container through the transferring connection.

Systems and methods for automatically packing resin into and unpacking resin from a reusable separation column for sample analysis are described. A method embodiment includes, but is not limited to, introducing a slurry of resin to a reusable sample separation column to pack the reusable column with resin; introducing a sample solution to the reusable sample separation column; and unpacking the resin from the reusable sample separation column with a flow of liquid unpacking reagent and gaseous material.

Provided herein in some embodiments is a column including a housing assembly and a cartridge assembly. The housing assembly can include a housing top, a housing bottom, and a housing siding. The housing siding can be fixedly coupled to the housing top and the housing bottom forming hermetic seals therebetween. The cartridge assembly can include a cartridge top, an outer frit, and an inner frit disposed within the outer frit. A toroidal space can defined by the cartridge top, the outer frit, the inner frit, and the housing bottom. The toroidal space can be configured to hold a stationary phase for radial flow column chromatography. Also provided herein in some embodiments is a process including assembling the cartridge assembly, assembling the housing assembly about the cartridge assembly, and pressure testing the column. In some embodiments, the process can further include charging the toroidal space with a stationary phase.

This disclosure provides chromatography columns that are packed with incompressible media such as ceramic hydroxyapatite particles, which exhibit high separation performance that is robust to transportation and multiple uses. The columns can be made by applying axial compression using rigid bodies, such as porous frits and/or flow regulators.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.