Supercritical fluid extraction can experience problems of prolonged extraction times and undesired elution of target components of a trap sample. To resolve these problems, a sample is stored in a container (125) disposed upstream of a back-pressure control valve (140). A mixed fluid of carbon dioxide in a supercritical state and a modifier is introduced into the container, and a component contained in the sample is extracted. The extracted component is introduced into a trap column (135) together with the carbon dioxide and the modifier and collected in the trap column. The trap column is loaded with polymer beads as a filler to securely collect an extracted component in the trap column, even when the modifier in the supercritical fluid is increased.

Provided is a guard column including a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, in which the filler is made of porous silica gel having a hydrophilized surface and an average particle size of 1.5 ?m to 2.5 ?m, and a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

A chromatographic bed insert includes a base having openings and an array of projection members positioned on the base and projecting substantially perpendicular to the base. The chromatographic bed insert has a displacement volume % D which is less than 50% of a volume defined by the chromatographic bed insert and is structured to reduce a hydraulic radius R.sub.H of a chromatography bed including the chromatographic bed insert by at least 25% compared to a corresponding chromatography bed which does not include the chromatographic bed insert.

Provided herein in some embodiments is a column including a housing assembly and a cartridge assembly. The housing assembly can include a housing top, a housing bottom, and a housing siding. The housing siding can be fixedly coupled to the housing top and the housing bottom forming hermetic seals therebetween. The cartridge assembly can include a cartridge top, an outer frit, and an inner frit disposed within the outer frit. A toroidal space can defined by the cartridge top, the outer frit, the inner frit, and the housing bottom. The toroidal space can be configured to hold a stationary phase for radial flow column chromatography. Also provided herein in some embodiments is a process including assembling the cartridge assembly, assembling the housing assembly about the cartridge assembly, and pressure testing the column. In some embodiments, the process can further include charging the toroidal space with a stationary phase.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

A chromatography column comprises a 2-axis inclinometer for determining or monitoring the orientation the axial verticality of the column tube or of a column component such as a piston. A column inclinometer may be mounted on the column. A piston inclinometer may be mounted on the piston. Outputs from the inclinometer may be fed to a programmable logic controller and used to control the operation of the column, e.g. by adjusting a support stand of the column, or controlling a piston drive mechanism.

In the specification, a method for purifying a compound by column chromatography, and a method for optimizing the symmetry factor and theoretical plate number by allowing the slurry to settle and reducing the sedimentation volume of the slurry. A compound purified by such method is also provided.

A method for packing a media bed in a column. According to the invention the method comprises the steps of: providing data to a control unit connected to the column, said data comprising at least a measured slurry concentration, a target bed height, a target packing factor or compression factor and minimum and maximum acceptable values for at least one of target bed height and target packing or compression factor; forcing a movable adapter along a longitudinal axis of the column in order to pack the media; detecting when the media bed is consolidated; the control unit processing the provided data and the information about the consolidated bed height in order to present to the user acceptable bed heights, if there are any, giving acceptable packing or compression factors.