Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

A method identifies glycopeptides in a sample. The method includes converting a mass spectrum of MS1 precursors of the sample into a plurality of nodes in a graph, each node corresponding to one mass and one retention time of a glycopeptide to be identified in the sample; calculating differences in the mass and/or retention time between all combinations of pairs of the nodes; generating a graph theoretic network of the nodes; and predicting compositions of the glycopeptides in the sample based on the graph theoretic network of the nodes so as to identify the glycopeptides.

A mixed-mode chromatography method for the determination of phosphorylated sugars in a sample is provided. The mixed-mode chromatography method includes obtaining a sample comprising at least one phosphorylated sugar. The sample is introduced onto a chromatography system. The chromatography system includes a column having a stationary phase material contained inside the column. The stationary phase material has a surface comprising a hydrophobic surface group and at least one ionizable modifier. The sample with a mobile phase eluent is flowed through the column, where the at least one phosphorylated sugar is substantially resolved and retained within seven minutes. The mobile phase eluent includes water with an additive and acetonitrile with the additive. The mobile phase eluent has a pH less than 6. The at least one phosphorylated sugar is detected using a detector.

Provided are methods for isolating biomolecules, such as nucleic acids and proteins, from a sample using a silica-containing surface and/or a high salt, low pH buffer.

A purification agent which includes a compound having a betaine structure, and which is for a sugar chain having a length equal to or longer than that of a monosaccharide or for a glycopeptide having a sugar chain having a length equal to or longer than that of a monosaccharide.

Solutions, detection methods and chromatographic systems are provided for electrospray ionization of glycans modified with amphipathic, strongly basic moieties. The solutions for use in electrospray ionization comprise a plurality of glycans having an amphipathic moiety, a basic residue of pKa>5 and a Log P value between 1 and 3, and one or more volatile components selected from the group consisting of an amine, ammonia, ammonia salt, diethylamine, or trimethylamine. The solutions also have a pH between about 3 to about 6, and ionic strength of between about 0 mM to about 500 mM. The solutions are useful in detecting modified glycans in electrospray ionization and in various chromatographic systems.

Disclosed is a method for decomposing chondroitin sulfate contained in a sample into disaccharide. In particular disclosed is a method for decomposing chondroitin sulfate contained in a sample into disaccharide by heating the chondroitin sulfate in HCl-methanol containing 2,2-dimethoxypropane at a temperature of 60° C. to 90° C. for 50 minutes to 180 minutes, optionally in the method, the sample is selected from body fluid, a cell, a tissue, an organ, a cell culture solution, a tissue culture solution, a food, and a feed, or a derived therefrom.

The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

A method identifies glycopeptides in a sample. The method includes converting a mass spectrum of MS1 precursors of the sample into a plurality of nodes in a graph, each node corresponding to one mass and one retention time of a glycopeptide to be identified in the sample; calculating differences in the mass and/or retention time between all combinations of pairs of the nodes; generating a graph theoretic network of the nodes; and predicting compositions of the glycopeptides in the sample based on the graph theoretic network of the nodes so as to identify the glycopeptides.

The present disclosure is related to the six isomer structures (OBI-821-1990-V1A, OBI-821-1990-V1B, OBI-821-1990-V2A, OBI-821-1990-V2B, OBI-821-1858-A, and OBI-821-1858-B) of isolated OBI-821 adjuvant and the method for evaluating the quality thereof. The method of the present disclosure adopts hydrophilic interaction liquid chromatography (HILIC) and reverse phase high performance liquid chromatography (RP-HPLC) either alone or in tandem and is able to separate the isomers of OBI-821 adjuvant in the consequent chromatography. Accordingly, the quality of OBI-821 adjuvant can be further evaluated.