A method of characterizing nanovesicles is disclosed. The method involves entrapping nanovesicles such as exosomes and sensing the dielectric properties of the exosomes using an electrical impedance sensing device. The method can distinguish exosomes based on different membrane compositions, different cellular origins, different size distribution and/or different cytosolic compositions.

The present invention includes methods and systems for optical assays, such as optogenetic assays, of biological activity in which an optical reference signal is used to normalize an optical test signal.

Disclosed is a cell imaging method including: forming a light sheet with respect to a flow cell; causing a measurement sample containing a plurality of cells to flow in the flow cell; and receiving lights generated from the plurality of cells passing through the light sheet, by an imaging device via an element configured to extend a depth of focus, and taking images of the plurality of cells by the imaging device.

A system for monitoring cell-substrate impedance of excitable cells at millisecond time resolution and methods of assessing cell beating by monitoring cell-substrate impedance of beating cells at millisecond time resolution.

Disclosed herein include systems, methods, compositions, and kits for measuring the secretion level of a secreted factor of a single cell. Disclosed herein include solid supports comprising a plurality of capture probes capable of specifically binding to secreted factors secreted by a single cell. In some of the embodiments, at least two of the capture probes are capable of binding different secreted factors. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe. Secreted factor-binding reagents can comprise a detectable moiety, or a precursor thereof. Secreted factor-binding reagents capable of binding the same secreted factor comprise the same detectable moiety, or a precursor thereof, and secreted factor-binding reagents capable of binding different secreted factors can comprise different detectable moieties, or precursors thereof.

Disclosed is an integrated biochemical sensor including a reference electrode, a plurality of working electrodes each having different artificial lipid membranes, and partition layers for electrically insulating the reference electrode and each of the working electrodes.

The invention, in some aspects relates to compositions and methods for altering cell activity and function and the introduction and use of light-activated ion channels.

A method of non-invasively detecting and purging bacterial cells using a modified photoacoustic in vivo flow cytometer device is described herein. In particular, a method of detecting bacterial cells by analyzing photoacoustic pulses emitted in response to laser pulses from a pulsed laser source and/or selectively destroying the detected bacterial cells using a non-linear photothermal response induced by a high-energy laser pulse is described herein.

The invention relates to a device for detecting and/or characterizing one or more cells by the electrical properties of the cells, the device comprising at least one electrode integrated microsieve assembly, wherein the assembly comprises a) a microsieve arrangement, such as a microsieve array, comprising one or more micropores for retaining the cells, and b) a substrate comprising one or more pairs of oppositely arranged first and second electrodes, wherein the microsieve arrangement is connected to the substrate such that each of the one or more pairs of electrodes is configured to form an electric field in at least one micropore of the microsieve arrangement, and wherein the first electrode is arranged in parallel to the second electrode.

A detection analyzer including a first sample input/output element, a second sample input/output element, a sample compartment, a vibration platform, a vibration generator, a data acquisition system, a laser converter, and a data display. The first sample input/output element and the second sample input/output element are each connected to the sample compartment; the vibration platform is located inside the sample compartment; the vibration generator is located outside the sample compartment, and the vibration platform is connected to the vibration generator; the data acquisition system is located outside the sample compartment, and is connected to the vibration platform; and the data display is connected to the data acquisition system.