A system for analyzing tissue includes a platform and an optical sensing unit coupled to the platform. The optical sensing unit has a detector and a plurality of light sources surrounding and electrically isolated from the detector. The optical sensing units obtain optical data for tissue analysis.

The present invention relates to diagnostic devices as well as methods of using these devices for detecting proteins of interest associated with diseases or disorders in mammals. In particular, the proteins of interest may be misfolded proteins associated with certain misfolded-protein disorders in mammals including those mammals suspected of or at risk of having such disorders.

A dry stick (180a, 180b, 180c) arranged to indicate at least one biomarker value of a milk sample of an animal (100) by a lateral flow test. The dry stick (180a, 180b, 180c) includes a sample pad (310) with a reagent, configured to indicate at least one biomarker value of a received milk sample of an animal (100) by changing colours when exposed for milk having the biomarker; a porous membrane (320), configured to create a capillary flow of the milk sample, from the sample pad (310) through the porous membrane (320); an absorbent pad (330) with an absorbent configured to absorb superfluous milk from the porous membrane (320); and a porous desiccant, configured to absorb moisture from environmental air.

The present disclosure relates to a bio-detection chip and a detection method associated therewith. The bio-detection chip includes an upper substrate, a lower substrate, a reference electrode, a driving electrode, and a first dielectric layer, a first hydrophobic layer, a second hydrophobic layer and a second dielectric layer disposed successively between the reference electrode and the driving electrode. The bio-detection chip further includes a plurality of micro-capsules arranged between the first hydrophobic layer and the second hydrophobic layer. Each micro-capsule encapsulates a plurality of charged microspheres, and surfaces of the charged microspheres have a first biomolecule for specifically binding with a second biomolecule that enters the bio-detection chip so as to give rise to a color change. The charged microspheres move close to the upper substrate when a voltage is applied between the reference electrode and the driving electrode. As such, a result of biomolecule detection may be observed intuitively.

A compact optical imaging system including a single filter and a light source that provides lateral illumination for bead detection in digital assays. The light source is configured to emit light toward the detection vessel. The single filter is positioned to receive light reflected from a sample in the detection vessel, that originated from the light source, and receive an output from a sample in the detection vessel. A detector is configured to receive a portion of the reflected light and a portion of the output that passes through the single filter.

A cartridge for determining a concentration of target cells within a sample includes a separation portion and a detection portion. The separation portion includes a first and second surface defining a separation chamber. The separation portion can contain a density medium having a density greater than a density of a first portion of the sample and less than a density of a second portion of the sample (that includes the target cells). The separation chamber can be fluidically coupled to an inlet reservoir such that the sample can pass from the inlet reservoir to the separation chamber during rotation. The detection portion includes a detection surface that forms a boundary of a detection chamber. The detection surface is nonparallel to the first surface such that the target cells impinge on the detection surface when passing into the detection chamber. The detection surface is configured to capture the target cells.

The application relates to proteome analysis in single cells. Specifically, disclosed are high throughput methods of detecting proteins in single cells using barcoding, aptamers and single cell sequencing. Solid supports used in recording the cell-of-origin of target proteins and target proteins expressed in the cell-of-origin are disclosed. Additionally, methods of detecting proteins and mRNA in single cells are disclosed. Additionally, methods of detecting protein interactions are disclosed. Additionally, methods of detecting post translationally modified proteins in single cells are disclosed. The application also relates to solid supports or beads and methods of producing said solid supports or beads for use in the described methods.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Generally, this disclosure relates to expression constructs that encode a reporter enzyme-affinity binding tag fusion protein that is produced after the construct is inserted into bacteriophage and the bacteriophage infects bacteria. In some embodiments, the fusion protein is captured and produces a detectable signal. Signal intensity may correlate with the number of bacterial cells in a fluid sample. Methods of detecting bacteria using the expression constructs, and microfluidic devices for detecting bacteria using the expression constructs are also disclosed.

Disclosed herein are methods of detecting microbial infection in mammalian subjects comprising treatment of a sample and detection of galactofuranose (galF)-containing antigenic components utilizing monoclonal antibodies. The methods disclosed provide for pretreatment of biological samples, such as urine samples, to maximize detection of galF antigens and improvement of sensitivity of galF antigen detection assays. The methods include minimizing intelectin-1 binding to galF antigens and improvement of monoclonal antibody binding. The detection methods are useful for identifying the presence of microbial antigens related to bacterial, fungal, and parasitic pathogens, including Streptococcus pneumoniae, Aspergillus species, Fusarium species, Coccidioides species, Cryptococcus species, Histoplasma species, and Leishmania species.