Described herein are methods, systems and devices for rapidly detecting biomarkers in a biological sample.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides an apparatus for detecting an analyte in a fluid sample, comprising a fluid sample collection element, comprising a fluid sample collection element that comprises an absorbing element; a carrier for accommodating a testing element; and a chamber for accommodating a testing element carrier, wherein the testing element carrier is located in the chamber, and the carrier further comprises an extrusion structure that is in contact with the absorbing element and extrudes the absorbing element.

A lateral filter array microfluidic (LFAM) device for highly efficient immunoaffinity isolation of target cells from a population of cells. The LFAM device may include of one or more serpentine main channels incorporated with lateral filter arrays. Antibodies are immobilized on the channel surface including the lateral filters and are capable of specific binding to one or more biomolecules on the surface of the target cell. The device may include one or more arrays of lateral filters with different sizes. The overall filters sizes are close to the diameter of the target cell, therefore the interaction between biomarkers on the target cells and corresponding antibodies immobilized on the filter surface is largely strengthened due to the direct contact between target cells and lateral filters. Methods include flowing a population of cells through an antibody-coated LFAM device for target cells capture, followed by washing the device to remove non-specific captured cells.

A method for the isolation of one or more target(s) of interest using fluidic devices with at least one inlet and at least one outlet; a multidirectional fluidic channel between the at least one inlet and the at least one outlet; said multidirectional fluidic channel comprising at least one wall; a substance coating at least a portion of an inside surface of the at least one wall that detects, captures, adsorbs, and/or removes the target(s) of interest from a sample; an eluant for eluting the materials of interest from the fluidic channel; concentrating a target sample from the eluted material(s) of interest and the subsequent analysis and identification of the eluted materials of interest and/or diagnose of a disease based on the target(s) of interest isolated from the sample.

Immunoassay devices with plurality of fluid flow channels that are discrete and designed for optimal fluid control are described. Substrates configured to control the rate of fluid flow for in-situ immunoassay measurements to detect and quantify the presence of one or more analytes of interest in a sample are also described. More specifically, the present disclosure relates to consumables for lateral flow assays, which in conjunction with an instrument detect markers or causative agents of medical conditions.

The optical analyte detector is a photonic detector that uses a measured wavelength shift to determine the presence of an analyte. An open cell is formed in an optical layer for receiving a sample to be analyzed. A transition metal dichalcogenide monolayer defines a bottom wall of the open cell, and the transition metal dichalcogenide monolayer is formed directly above a microring resonator. A waveguide is positioned adjacent to the open cell, and is spaced apart therefrom by a gap. The waveguide is coupled to the microring resonator, and the transition metal dichalcogenide monolayer is functionalized with an adsorbed layer for detection of a specific analyte. Molecular binding takes place if a sample of the analyte contacts the adsorbed layer, which induces a wavelength shift in light transmitted through the waveguide. The presence of this measured wavelength shift indicates positive detection of the analyte.

An ancillary saliva testing apparatus for the presence of a selected substance utilizing an internet connected electronic device with a camera for third party review, the electronic device also having electronic communication to the apparatus, the apparatus has an enclosure that accommodates an insertable saliva test element assembly that includes a test element casing that secures a saliva test strip within the casing. The enclosure includes color sensing circuitry for the saliva test strip and communication circuitry from the color sensing circuitry to the electronic device. Also, an user interface application in on the electronic device to document a user inserting the casing into the enclosure with the camera verifying the user identity via facial recognition, also the saliva test strip that includes both an identification code and change of state test result color code on the saliva test strip based on the user wetting the test strip with saliva.

This disclosure relates to compositions and methods for analyzing single cells using cell printing and spatial analysis.

The present disclosure provides devices, kits, and methods for focusing/enriching and/or separating/sorting submicron size particles, including biological entities such as exosomes and other submicron size extracellular vesicles. Devices, kits, and methods of the present disclosure utilize ferrohydrodynamic manipulation to focus populations of submicron particles into a stream for enrichment and/or further sort various sub-populations of submicron particles based on size differences.