Methods for single cell analysis by determining the presence and/or amount of one or more target molecules in a plurality of cells may include: (i) immobilizing said plurality of cells on a solid substrate, wherein the cells are immobilized in form of a monolayer; (ii) determining the position of the individual immobilized cells on the solid substrate; (iii) measuring the auto-fluorescence of the individual immobilized cells: (iv) contacting the immobilized cells with a first detection reagent comprising (a) a moiety that specifically recognizes and binds a first target molecule and (b) a fluorescent label under conditions that allow binding of the detection reagent to the first target molecule; (v) measuring the fluorescence of the fluorescent label of the detection reagent bound to the first target molecule for the individual immobilized cells; (vi) determining the presence and/or amount of the first target molecule in the individual immobilized cells by comparing the fluorescence measured in step(v) with the fluorescence measured in step (iii) on a cell-by-cell basis.

Immobilization zones through which a sample flows before reaching the proteolytic agents and/or active enzyme and associated electrode/s of an electrochemical test sensor may be used to trap or chemically deactivate active enzyme and/or proteolytic agent deactivating agents. Through either trapping or chemical deactivation, the immobilization zones substantially prevent the enzyme deactivating agents from reaching, and thus reducing the activity of the active enzyme or enzymes. Similarly, trapping or chemical deactivation may be used to prevent or substantially reduce the incidence of the proteolytic deactivating agent or agents from reaching the proteolytic agent.

According to one embodiment, a molecule detecting device includes a capturing section, a releasing section, and a detecting section. The capturing section is configured to, by combining a target molecule and a solubilizing agent with each other and thereby creating a composite body, capture the target molecule in a carrier liquid. The releasing section is configured to make the composite body release the target molecule therefrom in the carrier liquid. The detecting section is configured to carry out detection of the target molecule in the carrier liquid.

The present invention provides a microchannel structure loaded with a bead having a particle size for detecting whether a biological substance exists in a sample. The microchannel structure includes a structure body for passing a sample through the microchannel structure to have a test or a treatment. The structure body includes a sample entrance having a first aperture to allow the sample passing therethrough, a resistance-increasing section connected with the sample entrance, and having a second aperture being smaller than the first aperture, a detecting section connecting with the resistance-increasing section, and a bead mooring structure coupled to the second end for mooring the bead in the detecting section. The present invention can be used to capture rare cells in a biological system, such as human blood.

Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.

The invention relates to devices and methods for the detection of specific interactions between probe and target molecules. In particular, the invention relates to methods for the qualitative and/or quantitative detection of targets, including: introducing a sample containing targets into a reaction chamber formed between a first surface of the device and a second surface of a device, which is preferably located opposite to the first surface, wherein the distance between the first and the second surface is variable; and detecting the targets.

A method and composition for extracting an analyte from a test sample such as grain, so as to determine whether the test sample is contaminated with a toxin. The method is particularly useful for detecting the presence in a batch of grain of a mycotoxin, such as for example aflatoxin, ochratoxin, T2, zearalanone, vomitoxin (deoxynivalenol a/k/a DON), patulin and fumonisin. Extraction is performed with use of a composition that includes a proteinaceous material, such as albumin, as an extraction agent.

An immunoassay test slide for use in a dry chemistry analytical instrument includes a slide housing or case formed from two matable sections—a slide cover piece and a slide bottom piece. The slide housing defines an interior cavity in which is situated a sheet-like porous carrier matrix. The slide cover piece has an opening formed through the thickness thereof to expose a central portion of the fluid flow matrix so that a precise volume of fluid sample of blood, serum or the like, preferably pre-mixed with a conjugate reagent, and precise volumes of a wash reagent and a substrate (detector reagent), may be deposited on the matrix through the cover opening by a metering device of the analytical instrument. The bottom piece of the immunoassay test slide is transparent, and the slide is moved by a transport mechanism of the analytical instrument over a reflectometer or a fluorometer for performing reflectance or fluorescence measurements.

A system featuring a textured surface having elements, such as a plurality of microfeatures and/or microstructures, that increase the surface area as compared to a surface without the elements, wherein a non-volatile solvent is disposed in at least a portion of the elements of the textured surface. The non-volatile solvent may be used for retaining biomolecules, such as but not limited to biomolecules that may benefit from an environment that protects conformational structure, for example proteins.